Registro completo de metadatos
| Campo DC | Valor | Lengua/Idioma |
|---|---|---|
| dc.creator | Turina, Anahi del Valle | - |
| dc.creator | Schreier, Laura Ester | - |
| dc.creator | Perillo, Maria Angelica | - |
| dc.date | 2018-04-20T15:07:23Z | - |
| dc.date | 2018-04-20T15:07:23Z | - |
| dc.date | 2012-02 | - |
| dc.date | 2018-04-10T14:04:51Z | - |
| dc.date.accessioned | 2019-04-29T15:25:54Z | - |
| dc.date.available | 2019-04-29T15:25:54Z | - |
| dc.date.issued | 2012-02 | - |
| dc.identifier | Turina, Anahi del Valle; Schreier, Laura Ester; Perillo, Maria Angelica; Coupling between GABAA-R ligand- binding activity and membrane organization in β-cyclodextrin-treated synaptosomal membranes from bovine brain cortex: new insights from EPR experiments; Humana Press; Cell Biochemistry and Biophysics; 63; 1; 2-2012; 17-33 | - |
| dc.identifier | 1085-9195 | - |
| dc.identifier | http://hdl.handle.net/11336/42827 | - |
| dc.identifier | 1559-0283 | - |
| dc.identifier | CONICET Digital | - |
| dc.identifier | CONICET | - |
| dc.identifier.uri | http://rodna.bn.gov.ar:8080/jspui/handle/bnmm/294104 | - |
| dc.description | Correlations between GABAA-receptor (GABAA-R) activity and molecular organization of synaptosomal membranes (SM) were studied along the protocol for cholesterol (Cho) extraction with b-cyclodextrin (b-CD). The mere pre-incubation (PI) at 37ºC accompanying the b-CD treatment was an underlying source of perturbations increasing [3H]-FNZ maximal binding (70%) and Kd (38%), plus a stiffening of SMs hydrocarbon core region. The latter was inferred from an increased compressibility modulus (K) of SM-derived Langmuir films, a blue-shifted DPH fluorescence emission spectrum and the hysteresis in DPH fluorescence anisotropy (ADPH) in SMs submitted to a heating-cooling cycle (4ºC-37ºC-4ºC) with ADPH,heating<ADPH,cooling. Compared with PI-samples, the b-CD treatment reduced Bmax by 5% which correlated with a 45%-decrement in the relative Cho content of SM, a decrease in K and in the order parameter in the EPR spectrum of a lipid spin probe labeled at C5 (5-SASL), and significantly increased ATMA-DPH. PI, but not b-CD treatment, could affect the binding affinity. EPR spectra of 5-SASL complexes with b-CD-, SM-partitioned and free in solution showed that, contrary to what is usually assumed, b-CD is not completely eliminated from the system through centrifugation washings. It was concluded that, b-CD treatment involves effects of at least three different types of events affecting membrane organization: a) effect of PI on membrane annealing, b) effect of residual b-CD on SM organization and c) Cho depletion. Consequently, molecular stiffness increases within the membrane core and decreases near the polar head groups, leading to a net increase in GABAA-R density, relative to untreated samples. | - |
| dc.description | Fil: Turina, Anahi del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina | - |
| dc.description | Fil: Schreier, Laura Ester. Universidade de Sao Paulo; Brasil | - |
| dc.description | Fil: Perillo, Maria Angelica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.language | eng | - |
| dc.publisher | Humana Press | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs12013-012-9338-1 | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1007/s12013-012-9338-1 | - |
| dc.rights | info:eu-repo/semantics/restrictedAccess | - |
| dc.rights | https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ | - |
| dc.source | reponame:CONICET Digital (CONICET) | - |
| dc.source | instname:Consejo Nacional de Investigaciones Científicas y Técnicas | - |
| dc.source | instacron:CONICET | - |
| dc.subject | CYCLODEXTRIN | - |
| dc.subject | GABAA RECEPTOR | - |
| dc.subject | EPR | - |
| dc.subject | FLUORESCENCE ANISOTROPY | - |
| dc.subject | LANGMUIR FILMS | - |
| dc.subject | Otras Ciencias Biológicas | - |
| dc.subject | Ciencias Biológicas | - |
| dc.subject | CIENCIAS NATURALES Y EXACTAS | - |
| dc.title | Coupling between GABAA-R ligand- binding activity and membrane organization in β-cyclodextrin-treated synaptosomal membranes from bovine brain cortex: new insights from EPR experiments | - |
| dc.type | info:eu-repo/semantics/article | - |
| dc.type | info:eu-repo/semantics/publishedVersion | - |
| dc.type | info:ar-repo/semantics/articulo | - |
| Aparece en las colecciones: | CONICET | |
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