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dc.creatorHelguera, Gustavo Fernando-
dc.creatorRodríguez, José A.-
dc.creatorLuria Pérez, Rosendo-
dc.creatorHenery, Shannon-
dc.creatorCatterton, Paul-
dc.creatorBregni, Carlos-
dc.creatorGeorge, Thaddeus C.-
dc.creatorMartínez Maza, Otoniel-
dc.creatorPenichet, Manuel L.-
dc.date2017-02-13T15:51:34Z-
dc.date2017-02-13T15:51:34Z-
dc.date2011-05-
dc.date2017-02-09T18:23:26Z-
dc.date.accessioned2019-04-29T15:26:48Z-
dc.date.available2019-04-29T15:26:48Z-
dc.date.issued2011-05-
dc.identifierHelguera, Gustavo Fernando; Rodríguez, José A.; Luria Pérez, Rosendo; Henery, Shannon; Catterton, Paul; et al.; Visualization and quantification of cytotoxicity mediated by antibodies using imaging flow cytometry; Elsevier Science; Journal Of Immunological Methods; 368; 1-2; 5-2011; 54-63-
dc.identifier0022-1759-
dc.identifierhttp://hdl.handle.net/11336/12910-
dc.identifier.urihttp://rodna.bn.gov.ar:8080/jspui/handle/bnmm/294305-
dc.descriptionConventional approaches for the detection of antibody dependent cell-mediated cytotoxicity (ADCC) activity rely on quantification of the release of traceable compounds from target cells or flow cytometry analysis of population-wide phenomena. We report a new method for the direct imaging and quantification of ADCC of cancer cells. The proposed method using imaging flow cytometry combines the statistical power of flow cytometry with the analytical advantages of cell imaging, providing a novel and more comprehensive perspective of effector/target cell interactions during ADCC events. With this method we can quantify and show in detail the morphological changes in target and effector cells, their apoptotic index, the physical interaction between effector and target cells, and a directional transfer of cytosolic contents from effector to target cells. As a model system we used the therapeutic anti-CD20 antibody rituximab to target CFSE labeled Ramos human Burkitt's lymphoma cells, to CMTPX-labeled human monocytic U-937 effector cells. We expect that similar studies using different effector and target cell populations may contribute to the pre-clinical evaluation of therapeutic antibodies and help to identify mechanisms that could be beneficial in the immunotherapy of cancer.-
dc.descriptionFil: Helguera, Gustavo Fernando. University of California; Estados Unidos. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina-
dc.descriptionFil: Rodríguez, José A.. University of California; Estados Unidos-
dc.descriptionFil: Luria Pérez, Rosendo. University of California; Estados Unidos. Hospital infantil de México “Federico Gómez”; México-
dc.descriptionFil: Henery, Shannon. Amnis Corporation; Estados Unidos-
dc.descriptionFil: Catterton, Paul. Amnis Corporation; Estados Unidos-
dc.descriptionFil: Bregni, Carlos . Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina-
dc.descriptionFil: George, Thaddeus C.. Amnis Corporation; Estados Unidos-
dc.descriptionFil: Martínez Maza, Otoniel. University of California; Estados Unidos-
dc.descriptionFil: Penichet, Manuel L.. University of California; Estados Unidos-
dc.formatapplication/pdf-
dc.formatapplication/pdf-
dc.languageeng-
dc.publisherElsevier Science-
dc.relationinfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0022175911000585-
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.jim.2011.03.003-
dc.rightsinfo:eu-repo/semantics/restrictedAccess-
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/-
dc.sourcereponame:CONICET Digital (CONICET)-
dc.sourceinstname:Consejo Nacional de Investigaciones Científicas y Técnicas-
dc.sourceinstacron:CONICET-
dc.subjectAntibody-
dc.subjectADCC-
dc.subjectCancer-
dc.subjectImaging flow cytometry-
dc.subjectImmunotherapy-
dc.subjectOtras Biotecnologías de la Salud-
dc.subjectBiotecnología de la Salud-
dc.subjectCIENCIAS MÉDICAS Y DE LA SALUD-
dc.titleVisualization and quantification of cytotoxicity mediated by antibodies using imaging flow cytometry-
dc.typeinfo:eu-repo/semantics/article-
dc.typeinfo:eu-repo/semantics/publishedVersion-
dc.typeinfo:ar-repo/semantics/articulo-
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