Registro completo de metadatos
| Campo DC | Valor | Lengua/Idioma |
|---|---|---|
| dc.provenance | CONICET | - |
| dc.creator | Rojas, Hernan Javier | - |
| dc.creator | Floyd, Brice | - |
| dc.creator | Morriss, Stephanie C. | - |
| dc.creator | Bassham, Diane | - |
| dc.creator | MacIntosh, Gustavo C. | - |
| dc.creator | Goldraij, Ariel | - |
| dc.date | 2018-04-20T13:44:50Z | - |
| dc.date | 2018-04-20T13:44:50Z | - |
| dc.date | 2015-07 | - |
| dc.date | 2018-04-11T15:16:10Z | - |
| dc.date.accessioned | 2019-04-29T15:27:06Z | - |
| dc.date.available | 2019-04-29T15:27:06Z | - |
| dc.date.issued | 2018-04-20T13:44:50Z | - |
| dc.date.issued | 2018-04-20T13:44:50Z | - |
| dc.date.issued | 2015-07 | - |
| dc.date.issued | 2018-04-11T15:16:10Z | - |
| dc.identifier | Rojas, Hernan Javier; Floyd, Brice; Morriss, Stephanie C.; Bassham, Diane; MacIntosh, Gustavo C.; et al.; NnSR1, a class III non-S-RNase specifically induced in Nicotiana alata under phosphate deficiency, is localized in endoplasmic reticulum compartments; Elsevier Ireland; Plant Science; 236; 7-2015; 250-259 | - |
| dc.identifier | 0168-9452 | - |
| dc.identifier | http://hdl.handle.net/11336/42800 | - |
| dc.identifier | CONICET Digital | - |
| dc.identifier | CONICET | - |
| dc.identifier.uri | http://rodna.bn.gov.ar:8080/jspui/handle/bnmm/294389 | - |
| dc.description | A combined strategy of phosphate (Pi) remobilization from internal and external RNA sources seems to be conserved in plants exposed to Pi starvation. Thus far, the only ribonucleases (RNases) reported to be induced in Nicotiana alata undergoing Pi deprivation are extracellular S-like RNase NE and NnSR1. NnSR1 is a class III non S-RNase of unknown subcellular location. Here, we examine the hypothesis that NnSR1 is an intracellular RNase derived from the self-incompatibility system with specific expression in self-incompatible Nicotiana alata. NnSR1 was not induced in self-compatible Nicotiana species exposed to Pi deprivation. NnSR1 conjugated with a fluorescent protein and transiently expressed in Arabidopsis protoplasts and Nicotiana leaves showed that the fusion protein co-localized with an endoplasmic reticulum (ER) marker. Subcellular fractionation by ultracentrifugation of roots exposed to Pi deprivation revealed that the native NnSR1 migrated in parallel with the BiP protein, a typical ER marker. To our knowledge, NnSR1 is the first class III RNase reported to be localized in ER compartments. The induction of NnSR1 was detected earlier than the extracellular RNase NE, suggesting that intracellular RNA may be the first source of Pi used by the cell under Pi stress. | - |
| dc.description | Fil: Rojas, Hernan Javier. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Química Biológica; Argentina | - |
| dc.description | Fil: Floyd, Brice. University of Iowa; Estados Unidos | - |
| dc.description | Fil: Morriss, Stephanie C.. University of Iowa; Estados Unidos | - |
| dc.description | Fil: Bassham, Diane. University of Iowa; Estados Unidos | - |
| dc.description | Fil: MacIntosh, Gustavo C.. University of Iowa; Estados Unidos | - |
| dc.description | Fil: Goldraij, Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Química Biológica; Argentina | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.language | eng | - |
| dc.publisher | Elsevier Ireland | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1016/j.plantsci.2015.04.012 | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0168945215001120 | - |
| dc.rights | info:eu-repo/semantics/restrictedAccess | - |
| dc.rights | https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ | - |
| dc.source | reponame:CONICET Digital (CONICET) | - |
| dc.source | instname:Consejo Nacional de Investigaciones Científicas y Técnicas | - |
| dc.source | instacron:CONICET | - |
| dc.source.uri | http://hdl.handle.net/11336/42800 | - |
| dc.subject | Subcellular localization | - |
| dc.subject | Self-incompatibility | - |
| dc.subject | Endoplasmic reticulum | - |
| dc.subject | Phosphate starvation | - |
| dc.subject | Ribonuclease T2 | - |
| dc.subject | Otras Ciencias Biológicas | - |
| dc.subject | Ciencias Biológicas | - |
| dc.subject | CIENCIAS NATURALES Y EXACTAS | - |
| dc.title | NnSR1, a class III non-S-RNase specifically induced in Nicotiana alata under phosphate deficiency, is localized in endoplasmic reticulum compartments | - |
| dc.type | info:eu-repo/semantics/article | - |
| dc.type | info:eu-repo/semantics/publishedVersion | - |
| dc.type | info:ar-repo/semantics/articulo | - |
| Aparece en las colecciones: | CONICET | |
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