1. RODNA
  2. CONICET
  3. CONICET
Registro completo de metadatos
Campo DC Valor Lengua/Idioma
dc.creatorBevacqua, Romina Jimena-
dc.creatorFernández y Martín, Rafael-
dc.creatorSalamone, Daniel Felipe-
dc.date2017-05-02T19:02:01Z-
dc.date2017-05-02T19:02:01Z-
dc.date2011-04-
dc.date2017-04-24T20:43:03Z-
dc.date.accessioned2019-04-29T15:27:29Z-
dc.date.available2019-04-29T15:27:29Z-
dc.date.issued2017-05-02T19:02:01Z-
dc.date.issued2017-05-02T19:02:01Z-
dc.date.issued2011-04-
dc.date.issued2017-04-24T20:43:03Z-
dc.identifierBevacqua, Romina Jimena; Fernández y Martín, Rafael; Salamone, Daniel Felipe; Bovine parthenogenotes produced by inhibition of first or second polar bodies emission; Inst Histol Embriol-conicet; Biocell; 35; 1; 4-2011; 1-7-
dc.identifier0327-9545-
dc.identifierhttp://hdl.handle.net/11336/15879-
dc.identifier.urihttp://rodna.bn.gov.ar:8080/jspui/handle/bnmm/294508-
dc.descriptionParthenogenetic embryos are an ethically acceptable alternative for the derivation of human embryonic stem cells. In this work, we propose a new strategy to produce bovine parthenogenetic embryos inhibiting the emission of the first polar body during in vitro maturation, and allowing the extrusion of the second polar body during oocyte activation. Cytochalasin B, an inhibitor of actin microfilaments, was employed during in vitro maturation to inhibit first polar body emission or during parthenogenetic activation to block second polar body emission. Only one polar body was inhibited in each strategy in order to keep the diploid chromosome set. In experiment 1, the effect of cytochalasin B on in vitro maturation of bovine oocytes was evaluated. Most oocytes (77%) were arrested at a meiotic stage characterized by the presence of a large internal metaphase plate and absence of polar body. In experiment 2, development of embryos exposed to cytochalasin B during in vitro maturation (CytoB-IVM) or during activation (CytoB-ACT) was compared. Developmental rates did not differ between diploidization strategies, even when three agents were employed to induce activation. Both groups, CytoB-IVM and CytoB-ACT, tended to maintain diploidy. CytoB-IVM parthenogenesis could help to obtain embryos with a higher degree of homology to the oocyte donor.-
dc.descriptionFil: Bevacqua, Romina Jimena. Universidad de Buenos Aires. Facultad de Agronomía. Pabellón de Zootecnica. Laboratorio de Biotecnología Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina-
dc.descriptionFil: Fernández y Martín, Rafael. Universidad de Buenos Aires. Facultad de Agronomía. Pabellón de Zootecnica. Laboratorio de Biotecnología Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina-
dc.descriptionFil: Salamone, Daniel Felipe. Universidad de Buenos Aires. Facultad de Agronomía. Pabellón de Zootecnica. Laboratorio de Biotecnología Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina-
dc.formatapplication/pdf-
dc.formatapplication/pdf-
dc.formatapplication/pdf-
dc.formatapplication/pdf-
dc.languageeng-
dc.publisherInst Histol Embriol-conicet-
dc.relationinfo:eu-repo/semantics/altIdentifier/url/http://ref.scielo.org/jhnxpn-
dc.rightsinfo:eu-repo/semantics/openAccess-
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/-
dc.sourcereponame:CONICET Digital (CONICET)-
dc.sourceinstname:Consejo Nacional de Investigaciones Científicas y Técnicas-
dc.sourceinstacron:CONICET-
dc.subjectOocyte maturation-
dc.subjectMeiosis-
dc.subjectParthenogenesis-
dc.subjectFisiología-
dc.subjectMedicina Básica-
dc.subjectCIENCIAS MÉDICAS Y DE LA SALUD-
dc.titleBovine parthenogenotes produced by inhibition of first or second polar bodies emission-
dc.typeinfo:eu-repo/semantics/article-
dc.typeinfo:eu-repo/semantics/publishedVersion-
dc.typeinfo:ar-repo/semantics/articulo-
Aparece en las colecciones: CONICET

Ficheros en este ítem:
No hay ficheros asociados a este ítem.
Mostrar el registro sencillo del ítem