Registro completo de metadatos
| Campo DC | Valor | Lengua/Idioma |
|---|---|---|
| dc.creator | Sánchez Azqueta, Ana | - |
| dc.creator | Catalano Dupuy, Daniela Luján | - |
| dc.creator | Lopez Rivero, Arleth Susana | - |
| dc.creator | Tondo, Maria Laura | - |
| dc.creator | Orellano, Elena Graciela | - |
| dc.creator | Ceccarelli, Eduardo Augusto | - |
| dc.creator | Medina, Milagros | - |
| dc.date | 2017-12-05T13:13:25Z | - |
| dc.date | 2017-12-05T13:13:25Z | - |
| dc.date | 2014-06 | - |
| dc.date | 2017-12-04T19:16:45Z | - |
| dc.date.accessioned | 2019-04-29T15:30:46Z | - |
| dc.date.available | 2019-04-29T15:30:46Z | - |
| dc.date.issued | 2014-06 | - |
| dc.identifier | Sánchez Azqueta, Ana; Catalano Dupuy, Daniela Luján; Lopez Rivero, Arleth Susana; Tondo, Maria Laura; Orellano, Elena Graciela; et al.; Dynamics of the active site architecture in plant-type ferredoxin-NADP+ reductases catalytic complexes; Elsevier Science; Biochimica Et Biophysica Acta-bioenergetics; 1837; 10; 6-2014; 1730-1738 | - |
| dc.identifier | 0005-2728 | - |
| dc.identifier | http://hdl.handle.net/11336/29679 | - |
| dc.identifier | CONICET Digital | - |
| dc.identifier | CONICET | - |
| dc.identifier.uri | http://rodna.bn.gov.ar:8080/jspui/handle/bnmm/295468 | - |
| dc.description | Kinetic isotope effects in reactions involving hydride transfer and their temperature dependence are powerful tools to explore dynamics of enzyme catalytic sites. In plant-type ferredoxin-NADP+ reductases the FAD cofactor exchanges a hydride with the NADP(H) coenzyme. Rates for these processes are considerably faster for the plastidic members (FNR) of the family than for those belonging to the bacterial class (FPR). Hydride transfer (HT) and deuteride transfer (DT) rates for the NADP+ coenzyme reduction of four plant-type FNRs (two representatives of the plastidic type FNRs and the other two from the bacterial class), and their temperature dependences are here examined applying a full tunnelling model with coupled environmental fluctuations. Parameters for the two plastidic FNRs confirm a tunnelling reaction with active dynamics contributions, but isotope effects on Arrhenius factors indicate a larger contribution for donor–acceptor distance (DAD) dynamics in the Pisum sativum FNR reaction than in the Anabaena FNR reaction. On the other hand, parameters for bacterial FPRs are consistent with passive environmental reorganisation movements dominating the HT coordinate and no contribution of DAD sampling or gating fluctuations. This indicates that active sites of FPRs are more organised and rigid than those of FNRs. These differences must be due to adaptation of the active sites and catalytic mechanisms to fulfil their particular metabolic roles, establishing a compromise between protein flexibility and functional optimisation. Analysis of site-directed mutants in plastidic enzymes additionally indicates the requirement of a minimal optimal architecture in the catalytic complex to provide a favourable gating contribution | - |
| dc.description | Fil: Sánchez Azqueta, Ana. Universidad de Zaragoza; España | - |
| dc.description | Fil: Catalano Dupuy, Daniela Luján. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina | - |
| dc.description | Fil: Lopez Rivero, Arleth Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina | - |
| dc.description | Fil: Tondo, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina | - |
| dc.description | Fil: Orellano, Elena Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina | - |
| dc.description | Fil: Ceccarelli, Eduardo Augusto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina | - |
| dc.description | Fil: Medina, Milagros. Universidad de Zaragoza; España | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.language | eng | - |
| dc.publisher | Elsevier Science | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0005272814005118 | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.bbabio.2014.06.003 | - |
| dc.rights | info:eu-repo/semantics/restrictedAccess | - |
| dc.rights | https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ | - |
| dc.source | reponame:CONICET Digital (CONICET) | - |
| dc.source | instname:Consejo Nacional de Investigaciones Científicas y Técnicas | - |
| dc.source | instacron:CONICET | - |
| dc.subject | FERREDOXIN-NADP+ REDUCTASE | - |
| dc.subject | FLAVOENZYME | - |
| dc.subject | KINETIC ISOTOPE EFFECT | - |
| dc.subject | HYDRIDE TRANSFER | - |
| dc.subject | CHARGE-TRANSFER COMPLEX | - |
| dc.subject | PLASTIDIC-TYPE FNR | - |
| dc.subject | BACTERIAL-TYPE FPR | - |
| dc.subject | Otras Ciencias Biológicas | - |
| dc.subject | Ciencias Biológicas | - |
| dc.subject | CIENCIAS NATURALES Y EXACTAS | - |
| dc.title | Dynamics of the active site architecture in plant-type ferredoxin-NADP+ reductases catalytic complexes | - |
| dc.type | info:eu-repo/semantics/article | - |
| dc.type | info:eu-repo/semantics/publishedVersion | - |
| dc.type | info:ar-repo/semantics/articulo | - |
| Aparece en las colecciones: | CONICET | |
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