Registro completo de metadatos
| Campo DC | Valor | Lengua/Idioma |
|---|---|---|
| dc.creator | Peixoto Cupello, Mauricio | - |
| dc.creator | Souza, Cíntia Fernandes de | - |
| dc.creator | Buchensky, Celeste | - |
| dc.creator | Soares, Juliana Baptista Rocha Corrêa | - |
| dc.creator | Laranja, Gustavo Augusto Travasso | - |
| dc.creator | Coelho, Marsen Garcia Pinto | - |
| dc.creator | Cricco, Julia Alejandra | - |
| dc.creator | Paes, Marcia Cristina | - |
| dc.date | 2017-04-19T18:10:35Z | - |
| dc.date | 2017-04-19T18:10:35Z | - |
| dc.date | 2011-12 | - |
| dc.date | 2017-04-18T14:01:45Z | - |
| dc.date.accessioned | 2019-04-29T15:30:47Z | - |
| dc.date.available | 2019-04-29T15:30:47Z | - |
| dc.date.issued | 2011-12 | - |
| dc.identifier | Peixoto Cupello, Mauricio; Souza, Cíntia Fernandes de; Buchensky, Celeste; Soares, Juliana Baptista Rocha Corrêa; Laranja, Gustavo Augusto Travasso; et al.; The heme uptake process in Trypanosoma cruzi epimastigotes is inhibited by heme analogues and by inhibitors of ABC transporters; Elsevier Science; Acta Tropica; 120; 3; 12-2011; 211-218 | - |
| dc.identifier | 0001-706X | - |
| dc.identifier | http://hdl.handle.net/11336/15449 | - |
| dc.identifier.uri | http://rodna.bn.gov.ar:8080/jspui/handle/bnmm/295478 | - |
| dc.description | Heme (iron protoporphyrin IX) is an important molecule involved in many biological reactions, including oxygen transport, respiration, photosynthesis and drug detoxification. Trypanosoma cruzi parasites, the etiological agent of Chagas’ disease, take up heme from the environment to supply their nutritional needs because they do not synthesize this cofactor. However, the mechanisms involved in heme transport across biological membranes are poorly understood. Indeed, in T. cruzi, no heme transporter has yet been characterized. In the present work, we evaluate the heme uptake processes by T. cruzi epimastigotes using fluorescent heme-analogues. Heme uptake decreased significantly when cells were pretreated with different concentrations of SnPPIX, PdMPIX or ZnMPIX, this observed competition suggests that they are taken up by the same transport system. We studied the growth behavior of epimastigotes using the same heme-analogues and the treatments with SnPPIX or PdMPIX impaired cell growth but when heme was added to the culture medium the observed inhibition was partially reversed. In addition, we tested how the heme uptake processes are affected by the presence of different transporter inhibitors. When the cells were treated with inhibitors and then incubated with heme, heme uptake decreased significantly for all treatments. These results constitute a strong indication for the existence of a protein associated with porphyrin transport in T. cruzi, possibly ATP-binding cassette transporters (ABC-transporter). | - |
| dc.description | Fil: Peixoto Cupello, Mauricio. Universidade Do Estado de Rio Do Janeiro; Brasil | - |
| dc.description | Fil: Souza, Cíntia Fernandes de. Universidade Do Estado de Rio Do Janeiro; Brasil | - |
| dc.description | Fil: Buchensky, Celeste. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina | - |
| dc.description | Fil: Soares, Juliana Baptista Rocha Corrêa. Universidade Federal do Rio de Janeiro; Brasil | - |
| dc.description | Fil: Laranja, Gustavo Augusto Travasso. Universidade Do Estado de Rio Do Janeiro; Brasil | - |
| dc.description | Fil: Coelho, Marsen Garcia Pinto. Universidade Do Estado de Rio Do Janeiro; Brasil | - |
| dc.description | Fil: Cricco, Julia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina | - |
| dc.description | Fil: Paes, Marcia Cristina. Universidade Do Estado de Rio Do Janeiro; Brasil. Instituto Nacional de Ciência e Tecnologia; Brasil | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.language | eng | - |
| dc.publisher | Elsevier Science | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.actatropica.2011.08.011 | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0001706X11002531 | - |
| dc.rights | info:eu-repo/semantics/restrictedAccess | - |
| dc.rights | https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ | - |
| dc.source | reponame:CONICET Digital (CONICET) | - |
| dc.source | instname:Consejo Nacional de Investigaciones Científicas y Técnicas | - |
| dc.source | instacron:CONICET | - |
| dc.subject | HEME | - |
| dc.subject | TRYPANOSOMA CRUZI | - |
| dc.subject | PORPHYRINS | - |
| dc.subject | HEME TRANSPORT | - |
| dc.subject | Bioquímica y Biología Molecular | - |
| dc.subject | Ciencias Biológicas | - |
| dc.subject | CIENCIAS NATURALES Y EXACTAS | - |
| dc.title | The heme uptake process in Trypanosoma cruzi epimastigotes is inhibited by heme analogues and by inhibitors of ABC transporters | - |
| dc.type | info:eu-repo/semantics/article | - |
| dc.type | info:eu-repo/semantics/publishedVersion | - |
| dc.type | info:ar-repo/semantics/articulo | - |
| Aparece en las colecciones: | CONICET | |
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