Registro completo de metadatos
| Campo DC | Valor | Lengua/Idioma |
|---|---|---|
| dc.creator | Nicola, Juan Pablo | - |
| dc.creator | Reyna-Neyra, Andrea | - |
| dc.creator | Carrasco, Nancy | - |
| dc.creator | Masini, Ana María | - |
| dc.date | 2018-08-10T14:31:06Z | - |
| dc.date | 2018-08-10T14:31:06Z | - |
| dc.date | 2012-12 | - |
| dc.date | 2018-08-08T18:16:35Z | - |
| dc.date.accessioned | 2019-04-29T15:30:55Z | - |
| dc.date.available | 2019-04-29T15:30:55Z | - |
| dc.date.issued | 2012-12 | - |
| dc.identifier | Nicola, Juan Pablo; Reyna-Neyra, Andrea; Carrasco, Nancy; Masini, Ana María; Dietary iodide controls its own absorption through post-transcriptional regulation of the intestinal Na+/I- symporter; Wiley Blackwell Publishing, Inc; The Journal Of Physiology; 590; 23; 12-2012; 6013-6026 | - |
| dc.identifier | 0022-3751 | - |
| dc.identifier | http://hdl.handle.net/11336/54953 | - |
| dc.identifier | 1469-7793 | - |
| dc.identifier | CONICET Digital | - |
| dc.identifier | CONICET | - |
| dc.identifier.uri | http://rodna.bn.gov.ar:8080/jspui/handle/bnmm/295526 | - |
| dc.description | Dietary I- absorption in the gastrointestinal tract is the first step in I- metabolism. Given that I- is an essential constituent of the thyroid hormones, its concentrating mechanism is of significant physiological importance. We recently described the expression of the Na+/I- symporter (NIS) on the apical surface of the intestinal epithelium as a central component of the I- absorption system and reported reduced intestinal NIS expression in response to an I--rich diet in vivo. Here, we evaluated the mechanism involved in the regulation of NIS expression by I- itself in enterocytes. Excess I- reduced NIS-mediated I- uptake in IEC-6 cells in a dose- and time-dependent fashion, which was correlated with a reduction of NIS expression at the plasma membrane. Perchlorate, a competitive inhibitor of NIS, prevented these effects, indicating that an increase in intracellular I- regulates NIS. Iodide induced rapid intracellular recruitment of plasma membrane NIS molecules and NIS protein degradation. Lower NIS mRNA levels were detected in response to I- treatment, although no transcriptional effect was observed. Interestingly, I- decreased NIS mRNA stability, affecting NIS translation. Heterologous green fluorescent protein-based reporter constructs revealed a significant repressive effect of the I--targeting NIS mRNA 3′ untranslated region. In conclusion, excess I- downregulates NIS expression in enterocytes by virtue of a complex mechanism. Our data suggest that I- regulates intestinal NIS mRNA expression at the post-transcriptional level as part of an autoregulatory effect of I- on its own metabolism. © 2012 The Authors. The Journal of Physiology © 2012 The Physiological Society. | - |
| dc.description | Fil: Nicola, Juan Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina | - |
| dc.description | Fil: Reyna-Neyra, Andrea. University of Yale. School of Medicine; Estados Unidos | - |
| dc.description | Fil: Carrasco, Nancy. University of Yale. School of Medicine; Estados Unidos | - |
| dc.description | Fil: Masini, Ana María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.language | eng | - |
| dc.publisher | Wiley Blackwell Publishing, Inc | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/url/https://physoc.onlinelibrary.wiley.com/doi/abs/10.1113/jphysiol.2012.241307 | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1113/jphysiol.2012.241307 | - |
| dc.rights | info:eu-repo/semantics/restrictedAccess | - |
| dc.rights | https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ | - |
| dc.source | reponame:CONICET Digital (CONICET) | - |
| dc.source | instname:Consejo Nacional de Investigaciones Científicas y Técnicas | - |
| dc.source | instacron:CONICET | - |
| dc.subject | Na/I SYMPORTER | - |
| dc.subject | SMALL INTESTINE | - |
| dc.subject | IODIDE ABSOPTION | - |
| dc.subject | POSTRANSCRIPTIONAL REGULATION | - |
| dc.subject | Otras Ciencias Biológicas | - |
| dc.subject | Ciencias Biológicas | - |
| dc.subject | CIENCIAS NATURALES Y EXACTAS | - |
| dc.title | Dietary iodide controls its own absorption through post-transcriptional regulation of the intestinal Na+/I- symporter | - |
| dc.type | info:eu-repo/semantics/article | - |
| dc.type | info:eu-repo/semantics/publishedVersion | - |
| dc.type | info:ar-repo/semantics/articulo | - |
| Aparece en las colecciones: | CONICET | |
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