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Campo DC Valor Lengua/Idioma
dc.creatorAlvarez, Cora Lilia-
dc.creatorSchachter, Julieta-
dc.creatorSá Pinheiro, Ana Acacia de-
dc.creatorVerstraeten, Sandra Viviana-
dc.creatorSilva, Leandro de Souza-
dc.creatorMuanis Persechini, Pedro-
dc.creatorSchwarzbaum, Pablo Julio-
dc.date2017-06-13T16:52:40Z-
dc.date2017-06-13T16:52:40Z-
dc.date2014-05-
dc.date2017-06-09T15:02:32Z-
dc.date.accessioned2019-04-29T15:31:06Z-
dc.date.available2019-04-29T15:31:06Z-
dc.date.issued2014-05-
dc.identifierAlvarez, Cora Lilia; Schachter, Julieta; Sá Pinheiro, Ana Acacia de; Verstraeten, Sandra Viviana; Silva, Leandro de Souza; et al.; Regulation of extracellular ATP in human erythrocytes infected with Plasmodium falciparum; Public Library Of Science; Plos One; 9; 5; 5-2014; 1-14; e96216-
dc.identifier1932-6203-
dc.identifierhttp://hdl.handle.net/11336/18093-
dc.identifier.urihttp://rodna.bn.gov.ar:8080/jspui/handle/bnmm/295592-
dc.descriptionIn human erythrocytes (h-RBCs) various stimuli induce increases in [cAMP] that trigger ATP release. The resulting pattern of extracellular ATP accumulation (ATPe kinetics) depends on both ATP release and ATPe degradation by ectoATPase activity. In this study we evaluated ATPe kinetics from primary cultures of h-RBCs infected with P. falciparum at various stages of infection (ring, trophozoite and schizont stages). A “3V” mixture containing isoproterenol (β-adrenergic agonist), forskolin (adenylate kinase activator) and papaverine (phosphodiesterase inhibitor) was used to induce cAMP-dependent ATP release. ATPe kinetics of r-RBCs (ring-infected RBCs), t-RBCs (trophozoite-infected RBCs) and s-RBCs (schizont-infected RBCs) showed [ATPe] to peak acutely to a maximum value followed by a slower time dependent decrease. In all intraerythrocytic stages, values of ΔATP1 (difference between [ATPe] measured 1 min post-stimulus and basal [ATPe]) increased nonlinearly with parasitemia (from 2 to 12.5%). Under 3V exposure, t-RBCs at parasitemia 94% (t94-RBCs) showed 3.8-fold higher ΔATP1 values than in h-RBCs, indicative of upregulated ATP release. Pre-exposure to either 100 µM carbenoxolone, 100 nM mefloquine or 100 µM NPPB reduced ΔATP1 to 83–87% for h-RBCs and 63–74% for t94-RBCs. EctoATPase activity, assayed at both low nM concentrations (300–900 nM) and 500 µM exogenous ATPe concentrations increased approx. 400-fold in t94-RBCs, as compared to h-RBCs, while intracellular ATP concentrations of t94-RBCs were 65% that of h-RBCs. In t94-RBCs, production of nitric oxide (NO) was approx. 7-fold higher than in h-RBCs, and was partially inhibited by L-NAME pre-treatment. In media with L-NAME, ΔATP1 values were 2.7-times higher in h-RBCs and 4.2-times higher in t94-RBCs, than without L-NAME. Results suggest that P. falciparum infection of h-RBCs strongly activates ATP release via Pannexin 1 in these cells. Several processes partially counteracted ATPe accumulation: an upregulated ATPe degradation, an enhanced NO production, and a decreased intracellular ATP concentration.-
dc.descriptionFil: Alvarez, Cora Lilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina. Universidade Federal do Rio de Janeiro; Brasil-
dc.descriptionFil: Schachter, Julieta. Universidade Federal do Rio de Janeiro; Brasil-
dc.descriptionFil: Sá Pinheiro, Ana Acacia de. Universidade Federal do Rio de Janeiro; Brasil-
dc.descriptionFil: Verstraeten, Sandra Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina-
dc.descriptionFil: Silva, Leandro de Souza. Universidade Federal do Rio de Janeiro; Brasil-
dc.descriptionFil: Muanis Persechini, Pedro. Universidade Federal do Rio de Janeiro; Brasil-
dc.descriptionFil: Schwarzbaum, Pablo Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina-
dc.formatapplication/pdf-
dc.formatapplication/pdf-
dc.formatapplication/pdf-
dc.languageeng-
dc.publisherPublic Library Of Science-
dc.relationinfo:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0096216-
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1371/journal.pone.0096216-
dc.relationinfo:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4032238/-
dc.rightsinfo:eu-repo/semantics/openAccess-
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/-
dc.sourcereponame:CONICET Digital (CONICET)-
dc.sourceinstname:Consejo Nacional de Investigaciones Científicas y Técnicas-
dc.sourceinstacron:CONICET-
dc.subjectEXTRACELLULAR ATP REGULATION-
dc.subjectECTONUCLEOTIDASES-
dc.subjectPANNEXIN-1-
dc.subjectNITRIC OXYDE SYNTHASE-
dc.subjectBiofísica-
dc.subjectCiencias Biológicas-
dc.subjectCIENCIAS NATURALES Y EXACTAS-
dc.titleRegulation of extracellular ATP in human erythrocytes infected with Plasmodium falciparum-
dc.typeinfo:eu-repo/semantics/article-
dc.typeinfo:eu-repo/semantics/publishedVersion-
dc.typeinfo:ar-repo/semantics/articulo-
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