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dc.creatorSamsa, Marcelo Mario Alejandro-
dc.creatorMondotte, Juan Alberto-
dc.creatorIglesias, Nestor Gabriel-
dc.creatorAssunção Miranda, Iranaia-
dc.creatorBarbosa Lima, Giselle-
dc.creatorDa Poian, Andrea T.-
dc.creatorBozza, Patricia T.-
dc.creatorGamarnik, Andrea Vanesa-
dc.date2017-10-04T19:45:43Z-
dc.date2017-10-04T19:45:43Z-
dc.date2009-10-
dc.date2017-09-27T17:16:25Z-
dc.date.accessioned2019-04-29T15:31:11Z-
dc.date.available2019-04-29T15:31:11Z-
dc.date.issued2009-10-
dc.identifierSamsa, Marcelo Mario Alejandro; Mondotte, Juan Alberto; Iglesias, Nestor Gabriel; Assunção Miranda, Iranaia; Barbosa Lima, Giselle; et al.; Dengue virus capsid protein usurps lipid droplets for viral particle formation; Public Library of Science; Plos Pathogens; 5; 10; 10-2009; 1-14-
dc.identifier1553-7366-
dc.identifierhttp://hdl.handle.net/11336/25925-
dc.identifier1553-7374-
dc.identifierCONICET Digital-
dc.identifierCONICET-
dc.identifier.urihttp://rodna.bn.gov.ar:8080/jspui/handle/bnmm/295628-
dc.descriptionDengue virus is responsible for the highest rates of disease and mortality among the members of the Flavivirus genus. Dengue epidemics are still occurring around the world, indicating an urgent need of prophylactic vaccines and antivirals. In recent years, a great deal has been learned about the mechanisms of dengue virus genome amplification. However, little is known about the process by which the capsid protein recruits the viral genome during encapsidation. Here, we found that the mature capsid protein in the cytoplasm of dengue virus infected cells accumulates on the surface of ER-derived organelles named lipid droplets. Mutagenesis analysis using infectious dengue virus clones has identified specific hydrophobic amino acids, located in the center of the capsid protein, as key elements for lipid droplet association. Substitutions of amino acid L50 or L54 in the capsid protein disrupted lipid droplet targeting and impaired viral particle formation. We also report that dengue virus infection increases the number of lipid droplets per cell, suggesting a link between lipid droplet metabolism and viral replication. In this regard, we found that pharmacological manipulation of the amount of lipid droplets in the cell can be a means to control dengue virus replication. In addition, we developed a novel genetic system to dissociate cis-acting RNA replication elements from the capsid coding sequence. Using this system, we found that mislocalization of a mutated capsid protein decreased viral RNA amplification. We propose that lipid droplets play multiple roles during the viral life cycle; they could sequester the viral capsid protein early during infection and provide a scaffold for genome encapsidation.-
dc.descriptionFil: Samsa, Marcelo Mario Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina-
dc.descriptionFil: Mondotte, Juan Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina-
dc.descriptionFil: Iglesias, Nestor Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina-
dc.descriptionFil: Assunção Miranda, Iranaia. Universidade Federal do Rio de Janeiro; Brasil-
dc.descriptionFil: Barbosa Lima, Giselle. Escola Nacional de Saude Publica Sergio Arouca. Fundación Oswaldo Cruz; Brasil-
dc.descriptionFil: Da Poian, Andrea T.. Universidade Federal do Rio de Janeiro; Brasil-
dc.descriptionFil: Bozza, Patricia T.. Escola Nacional de Saude Publica Sergio Arouca. Fundación Oswaldo Cruz; Brasil-
dc.descriptionFil: Gamarnik, Andrea Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina-
dc.formatapplication/pdf-
dc.formatapplication/pdf-
dc.formatapplication/pdf-
dc.formatapplication/pdf-
dc.languageeng-
dc.publisherPublic Library of Science-
dc.relationinfo:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1000632-
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1371/journal.ppat.1000632-
dc.rightsinfo:eu-repo/semantics/openAccess-
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/-
dc.sourcereponame:CONICET Digital (CONICET)-
dc.sourceinstname:Consejo Nacional de Investigaciones Científicas y Técnicas-
dc.sourceinstacron:CONICET-
dc.subjectLipid droplets-
dc.subjectDengue virus-
dc.subjectEncapsidation-
dc.subjectBioquímica y Biología Molecular-
dc.subjectCiencias Biológicas-
dc.subjectCIENCIAS NATURALES Y EXACTAS-
dc.subjectBioquímica y Biología Molecular-
dc.subjectCiencias Biológicas-
dc.subjectCIENCIAS NATURALES Y EXACTAS-
dc.titleDengue virus capsid protein usurps lipid droplets for viral particle formation-
dc.typeinfo:eu-repo/semantics/article-
dc.typeinfo:eu-repo/semantics/publishedVersion-
dc.typeinfo:ar-repo/semantics/articulo-
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