Registro completo de metadatos
| Campo DC | Valor | Lengua/Idioma |
|---|---|---|
| dc.creator | Gavara, Poondi Rajesh | - |
| dc.creator | Cabrera, Rosa | - |
| dc.creator | Vennapusa, Rami Reddy | - |
| dc.creator | Grasselli, Mariano | - |
| dc.creator | Fernandez-Lahore, Marcelo | - |
| dc.date | 2019-01-08T20:43:16Z | - |
| dc.date | 2019-01-08T20:43:16Z | - |
| dc.date | 2012-08 | - |
| dc.date | 2019-01-02T19:31:58Z | - |
| dc.date.accessioned | 2019-04-29T15:31:19Z | - |
| dc.date.available | 2019-04-29T15:31:19Z | - |
| dc.date.issued | 2012-08 | - |
| dc.identifier | Gavara, Poondi Rajesh; Cabrera, Rosa; Vennapusa, Rami Reddy; Grasselli, Mariano; Fernandez-Lahore, Marcelo; Preparation, characterization, and process performance of composite fibrous adsorbents as cation exchangers for high throughput and high capacity bioseparations; Elsevier Science; Journal of Chromatography B; 903; 8-2012; 14-22 | - |
| dc.identifier | 0378-4347 | - |
| dc.identifier | http://hdl.handle.net/11336/67713 | - |
| dc.identifier | CONICET Digital | - |
| dc.identifier | CONICET | - |
| dc.identifier.uri | http://rodna.bn.gov.ar:8080/jspui/handle/bnmm/295683 | - |
| dc.description | Fibrous materials are proposed as novel chromatographic supports depicting high throughput and high product capacity. In this work, a composite fiber harboring strong cation-exchange moieties has been investigated. Such materials were characterized by a plethora of physical methods including degree of swelling (DS), scanning electron microscope (SEM), confocal laser scanning microscopy (CLSM), and Fourier transform infrared spectroscopy-attenuated total reflection (FTIR-ATR). The composite showed a high degree of grafting (∼30%) and exhibited a high swelling ratio (∼300%). Moreover, homogenous grafting and the development of an internal (functional) hydrogel were observed. The fibrous adsorbent was packed utilizing a designed " double roll" supported-structure and subsequently tested for packing efficiency and chromatography performance. The mentioned system showed similar packing efficiency of height equivalent to a theoretical plate (HETP) value and higher permeability coefficient (0.92×10 -7cm 2) than commercial resins. Experimentally determined Peclet number (Pe) values were within the range 60-90, suggesting a close-to-plug-flow condition. Total ionic capacity of the fibrous adsorbent was determined by the transition pH method. A capacity of 6.5mequiv./g was obtained. Moreover, a high dynamic binding capacity for lysozyme was found to be 283mg/g. On the other hand, a bed of randomly packed fiber also demonstrated high-resolution ability when a mixture of model protein was utilized to that end. Resolution was maintained at high flow rates (up to 900cm/h) and utilizing shorter gradient development routines. Direct sequestration of a model protein (lysozyme) was also possible from an artificial mixture containing 1.5% yeast homogenate. Summarizing, the composite fibrous adsorbents exhibited superior performance during early protein capture and intermediate-resolution applications. | - |
| dc.description | Fil: Gavara, Poondi Rajesh. Universitat Bremen; Alemania | - |
| dc.description | Fil: Cabrera, Rosa. Universitat Bremen; Alemania | - |
| dc.description | Fil: Vennapusa, Rami Reddy. Universitat Bremen; Alemania | - |
| dc.description | Fil: Grasselli, Mariano. Universidad Nacional de Quilmes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina | - |
| dc.description | Fil: Fernandez-Lahore, Marcelo. Universitat Bremen; Alemania | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.language | eng | - |
| dc.publisher | Elsevier Science | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1570023212003728 | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.jchromb.2012.06.027 | - |
| dc.rights | info:eu-repo/semantics/restrictedAccess | - |
| dc.rights | https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ | - |
| dc.source | reponame:CONICET Digital (CONICET) | - |
| dc.source | instname:Consejo Nacional de Investigaciones Científicas y Técnicas | - |
| dc.source | instacron:CONICET | - |
| dc.subject | COLUMN PACKING | - |
| dc.subject | COMPOSITE FIBROUS ADSORBENT | - |
| dc.subject | DYNAMIC BINDING CAPACITY | - |
| dc.subject | HYDROGEL | - |
| dc.subject | PERMEABILITY COEFFICIENT | - |
| dc.subject | RESOLUTION FACTOR | - |
| dc.subject | Biotecnología Industrial | - |
| dc.subject | Biotecnología Industrial | - |
| dc.subject | INGENIERÍAS Y TECNOLOGÍAS | - |
| dc.title | Preparation, characterization, and process performance of composite fibrous adsorbents as cation exchangers for high throughput and high capacity bioseparations | - |
| dc.type | info:eu-repo/semantics/article | - |
| dc.type | info:eu-repo/semantics/publishedVersion | - |
| dc.type | info:ar-repo/semantics/articulo | - |
| Aparece en las colecciones: | CONICET | |
Ficheros en este ítem:
No hay ficheros asociados a este ítem.