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| Campo DC | Valor | Lengua/Idioma |
|---|---|---|
| dc.creator | Weissmann, Carina | - |
| dc.creator | Di Guilmi, Mariano Nicolás | - |
| dc.creator | Urbano Suarez, Francisco Jose | - |
| dc.creator | Uchitel, Osvaldo Daniel | - |
| dc.date | 2017-07-11T19:14:11Z | - |
| dc.date | 2017-07-11T19:14:11Z | - |
| dc.date | 2012-10 | - |
| dc.date | 2017-07-11T13:25:16Z | - |
| dc.date.accessioned | 2019-04-29T15:31:28Z | - |
| dc.date.available | 2019-04-29T15:31:28Z | - |
| dc.date.issued | 2012-10 | - |
| dc.identifier | Weissmann, Carina; Di Guilmi, Mariano Nicolás; Urbano Suarez, Francisco Jose; Uchitel, Osvaldo Daniel; Acute effects of pregabalin on the function and cellular distribution of CaV2.1 in HEK293t cells; Elsevier Inc; Brain Research Bulletin; 90; 10-2012; 107-113 | - |
| dc.identifier | 0361-9230 | - |
| dc.identifier | http://hdl.handle.net/11336/20141 | - |
| dc.identifier | CONICET Digital | - |
| dc.identifier | CONICET | - |
| dc.identifier.uri | http://rodna.bn.gov.ar:8080/jspui/handle/bnmm/295732 | - |
| dc.description | We established a cell model to study the acute effects of pregabalin (PGB), a drug widely used in epilepsy and neuropathic pain, on voltage gated CaV2.1 (P/Q-type) calcium channels function and distribution at the membrane level. HEK293t cells were transfected with plasmids coding for all subunits of the CaV2.1 channel. We used a α1 fused to an eGFP tag to follow its distribution in time and at different experimental conditions. The expressed channel was functional as shown by the presence of barium-mediated, calcium currents of transfected cells measured by ‘whole-cell voltage-clamp’ recordings, showing a maximum current peak in the I–V curve at +20 mV. The GFP fluorescent signal was confined to the periphery of the cells. Incubation with 500 μM PGB, that binds α2δ subunits, for 30 min induced changes in localization of the fluorescent subunits as measured by fluorescent time lapse microscopy. These changes correlated with a reversible reduction of barium currents through CaV2.1 calcium channels under the same conditions. However, no changes in the cellular distribution of the subunits were visualized for cells either expressing another membrane associated protein or after exposure of the CaV2.1 channels to isoleucine, another α2δ ligand. Together these results show strong evidence for an acute effect of PGB on CaV2.1 calcium channels’ currents and distribution and suggest that internalization of CaV2.1 channels might be a mechanism of PGB action. | - |
| dc.description | Fil: Weissmann, Carina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina | - |
| dc.description | Fil: Di Guilmi, Mariano Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina | - |
| dc.description | Fil: Urbano Suarez, Francisco Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina | - |
| dc.description | Fil: Uchitel, Osvaldo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.language | eng | - |
| dc.publisher | Elsevier Inc | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.brainresbull.2012.10.001 | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0361923012002183 | - |
| dc.rights | info:eu-repo/semantics/restrictedAccess | - |
| dc.rights | https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ | - |
| dc.source | reponame:CONICET Digital (CONICET) | - |
| dc.source | instname:Consejo Nacional de Investigaciones Científicas y Técnicas | - |
| dc.source | instacron:CONICET | - |
| dc.subject | PREGABALIN | - |
| dc.subject | CALCIUM CHANNELS | - |
| dc.subject | HEK293t CELLS | - |
| dc.subject | ENDOCYTOSIS | - |
| dc.subject | Neurociencias | - |
| dc.subject | Medicina Básica | - |
| dc.subject | CIENCIAS MÉDICAS Y DE LA SALUD | - |
| dc.title | Acute effects of pregabalin on the function and cellular distribution of CaV2.1 in HEK293t cells | - |
| dc.type | info:eu-repo/semantics/article | - |
| dc.type | info:eu-repo/semantics/publishedVersion | - |
| dc.type | info:ar-repo/semantics/articulo | - |
| Aparece en las colecciones: | CONICET | |
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