Registro completo de metadatos
| Campo DC | Valor | Lengua/Idioma |
|---|---|---|
| dc.creator | Kenney, Mary | - |
| dc.creator | Waters, Ryan A. | - |
| dc.creator | Rieder, Elizabeth | - |
| dc.creator | Pega, Juan Franco | - |
| dc.creator | Pérez Filgueira, Daniel Mariano | - |
| dc.creator | Golde, William T. | - |
| dc.date | 2018-04-06T17:44:34Z | - |
| dc.date | 2018-04-06T17:44:34Z | - |
| dc.date | 2017-07 | - |
| dc.date | 2018-04-06T14:09:15Z | - |
| dc.date.accessioned | 2019-04-29T15:31:44Z | - |
| dc.date.available | 2019-04-29T15:31:44Z | - |
| dc.date.issued | 2017-07 | - |
| dc.identifier | Kenney, Mary; Waters, Ryan A.; Rieder, Elizabeth; Pega, Juan Franco; Pérez Filgueira, Daniel Mariano; et al.; Enhanced sensitivity in detection of antiviral antibody responses using biotinylation of foot-and-mouth disease virus (FMDV) capsids; Elsevier Science; Journal Of Immunological Methods; 450; 7-2017; 1-9 | - |
| dc.identifier | 0022-1759 | - |
| dc.identifier | http://hdl.handle.net/11336/41173 | - |
| dc.identifier | CONICET Digital | - |
| dc.identifier | CONICET | - |
| dc.identifier.uri | http://rodna.bn.gov.ar:8080/jspui/handle/bnmm/295832 | - |
| dc.description | Analysis of the immune response to infection of livestock by foot-and-mouth disease virus (FMDV) is most often reported as the serum antibody response to the virus. While measurement of neutralizing antibody has been sensitive and specific, measurements of the quality of the antibody response are less robust. Determining the immunoglobulin (Ig) isotype of the serum antibody response provides a deeper understanding of the biology of the response and more sensitive methods for these assays will facilitate analyses of B cell mediated immunity. We tested the hypothesis that using the virus as the molecular probe could be achieved by adding tags to the surface of the FMDV capsid, and that would enhance sensitivity in assays for anti-FMDV antibody responses. The use of a FLAG-tagged virus in these assays failed to yield improvement whereas chemically biotinylating the virus capsid resulted in significant enhancement of the signal. Here we describe methods using biotinylated virus for measuring anti-viral antibody in serum and antibody secreting cells (ASCs) in blood that are sensitive and specific. Finally, we describe using the biotinylated virus in flow cytometry where such assays should greatly enhance the analysis of anti-virus antibody producing B cells, allowing the investigator to focus on only the FMDV specific B cells when analyzing the development of the B cell response to either infection or vaccination. | - |
| dc.description | Fil: Kenney, Mary. Agricultural Research Service; Estados Unidos | - |
| dc.description | Fil: Waters, Ryan A.. Agricultural Research Service; Estados Unidos | - |
| dc.description | Fil: Rieder, Elizabeth. Agricultural Research Service; Estados Unidos | - |
| dc.description | Fil: Pega, Juan Franco. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas; Argentina | - |
| dc.description | Fil: Pérez Filgueira, Daniel Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas; Argentina | - |
| dc.description | Fil: Golde, William T.. Agricultural Research Service; Estados Unidos | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.language | eng | - |
| dc.publisher | Elsevier Science | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.jim.2017.07.001 | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0022175917302144 | - |
| dc.rights | info:eu-repo/semantics/restrictedAccess | - |
| dc.rights | https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ | - |
| dc.source | reponame:CONICET Digital (CONICET) | - |
| dc.source | instname:Consejo Nacional de Investigaciones Científicas y Técnicas | - |
| dc.source | instacron:CONICET | - |
| dc.subject | B CELL RESPONSES | - |
| dc.subject | BIOTINYLATION | - |
| dc.subject | ELISA | - |
| dc.subject | ELISPOT | - |
| dc.subject | FLOW CYTOMETRY | - |
| dc.subject | FMDV | - |
| dc.subject | Otras Ciencias Veterinarias | - |
| dc.subject | Ciencias Veterinarias | - |
| dc.subject | CIENCIAS AGRÍCOLAS | - |
| dc.title | Enhanced sensitivity in detection of antiviral antibody responses using biotinylation of foot-and-mouth disease virus (FMDV) capsids | - |
| dc.type | info:eu-repo/semantics/article | - |
| dc.type | info:eu-repo/semantics/publishedVersion | - |
| dc.type | info:ar-repo/semantics/articulo | - |
| Aparece en las colecciones: | CONICET | |
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