Registro completo de metadatos
| Campo DC | Valor | Lengua/Idioma |
|---|---|---|
| dc.provenance | CONICET | - |
| dc.creator | Ginart, Santiago | - |
| dc.creator | Caputo, Mariela | - |
| dc.creator | Corach, Daniel | - |
| dc.creator | Sala, Adriana Andrea | - |
| dc.date | 2018-06-14T14:48:53Z | - |
| dc.date | 2018-06-14T14:48:53Z | - |
| dc.date | 2017-12 | - |
| dc.date | 2018-06-13T15:07:17Z | - |
| dc.date.accessioned | 2019-04-29T15:32:01Z | - |
| dc.date.available | 2019-04-29T15:32:01Z | - |
| dc.date.issued | 2018-06-14T14:48:53Z | - |
| dc.date.issued | 2018-06-14T14:48:53Z | - |
| dc.date.issued | 2017-12 | - |
| dc.date.issued | 2018-06-13T15:07:17Z | - |
| dc.identifier | Ginart, Santiago; Caputo, Mariela; Corach, Daniel; Sala, Adriana Andrea; Measuring human DNA degradation and gender detection in forensic DNA samples by q-PCR/HRM analysis; Elsevier Science; Forensic Science International: Genetics Supplement Series; 6; 12-2017; e552-e554 | - |
| dc.identifier | 1875-175X | - |
| dc.identifier | http://hdl.handle.net/11336/48625 | - |
| dc.identifier | CONICET Digital | - |
| dc.identifier | CONICET | - |
| dc.identifier.uri | http://rodna.bn.gov.ar:8080/jspui/handle/bnmm/295910 | - |
| dc.description | Human DNA quantification, DNA degradation assessment and gender determination are key aspects in most field of human DNA analysis. The assay reported here is a tri-plex Real Time quantitative PCR reaction followed by high resolution melting (HRM) using Syto9 as intercalating dye. The system produces three amplicons: 1- transducin (beta)-like 1, Y-linked −TBL1Y (84 bp), 2- DeGraded small target DNA–DGst- (152 bp) and 3-DeGraded large target DNA-DGlt- (244 bp). DNA quantitation is based on total fluorescence; TBL1Y amplicon allows detecting male DNA and the ratio DGst/DGlt to assess DNA degradation level. q-PCR quantitation proved good linearity in triplicates among 3.2 pg/ul–50 ng/ul DNA concentration range. Amplification efficiency (E) and reaction slope (m) mean values were 1.04 and 3.23 respectively. Upon HRM analysis, three melting peaks are detected in a male DNA sample and two if only female DNA is present. We define the parameter D as the ratio DGst/DGlt that reflects the extent of DNA degradation in a given sample. A direct correlation has been demonstrated between DNA damage and increased value of parameter D. This q-PCR approach is rapid, sensitive, and a cost-effective method suitable for detecting degraded DNA samples and applicable to any field where human DNA quantitation-qualification is required. | - |
| dc.description | Fil: Ginart, Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Servicio de Huellas Digitales Genéticas; Argentina | - |
| dc.description | Fil: Caputo, Mariela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Servicio de Huellas Digitales Genéticas; Argentina | - |
| dc.description | Fil: Corach, Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Servicio de Huellas Digitales Genéticas; Argentina | - |
| dc.description | Fil: Sala, Adriana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Servicio de Huellas Digitales Genéticas; Argentina | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.language | eng | - |
| dc.publisher | Elsevier Science | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1016/j.fsigss.2017.09.211 | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1875176817301038 | - |
| dc.rights | info:eu-repo/semantics/restrictedAccess | - |
| dc.rights | https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ | - |
| dc.source | reponame:CONICET Digital (CONICET) | - |
| dc.source | instname:Consejo Nacional de Investigaciones Científicas y Técnicas | - |
| dc.source | instacron:CONICET | - |
| dc.source.uri | http://hdl.handle.net/11336/48625 | - |
| dc.subject | DEGRADED DNA ASSESSMENT | - |
| dc.subject | DNA QUANTITATION | - |
| dc.subject | HIGH RESOLUTION MELTING ANALYSIS | - |
| dc.subject | MALE DNA DETECTION | - |
| dc.subject | Otras Ciencias Biológicas | - |
| dc.subject | Ciencias Biológicas | - |
| dc.subject | CIENCIAS NATURALES Y EXACTAS | - |
| dc.title | Measuring human DNA degradation and gender detection in forensic DNA samples by q-PCR/HRM analysis | - |
| dc.type | info:eu-repo/semantics/article | - |
| dc.type | info:eu-repo/semantics/publishedVersion | - |
| dc.type | info:ar-repo/semantics/articulo | - |
| Aparece en las colecciones: | CONICET | |
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