1. RODNA
  2. CONICET
  3. CONICET
Registro completo de metadatos
Campo DC Valor Lengua/Idioma
dc.creatorLoviso, Claudia Lorena-
dc.creatorLozada, Mariana-
dc.creatorGuibert, Lilian Marcela-
dc.creatorMusumeci, Matias Alejandro-
dc.creatorSarango Cardenas, Sandra-
dc.creatorKuin, Ruud V.-
dc.creatorMarcos, Magalí Silvina-
dc.creatorDionisi, Hebe Monica-
dc.date2017-07-21T18:48:48Z-
dc.date2017-07-21T18:48:48Z-
dc.date2015-06-16-
dc.date2017-07-20T14:21:08Z-
dc.date.accessioned2019-04-29T15:32:18Z-
dc.date.available2019-04-29T15:32:18Z-
dc.date.issued2017-07-21T18:48:48Z-
dc.date.issued2017-07-21T18:48:48Z-
dc.date.issued2015-06-16-
dc.date.issued2017-07-20T14:21:08Z-
dc.identifierLoviso, Claudia Lorena; Lozada, Mariana; Guibert, Lilian Marcela; Musumeci, Matias Alejandro; Sarango Cardenas, Sandra; et al.; Metagenomics reveals the high polycyclic aromatic hydrocarbon-degradation potential of abundant uncultured bacteria from chronically polluted subantarctic and temperate coastal marine environments; Wiley Blackwell Publishing, Inc; Journal of Applied Microbiology; 119; 2; 16-6-2015; 411–424-
dc.identifier1364-5072-
dc.identifierhttp://hdl.handle.net/11336/21103-
dc.identifierCONICET Digital-
dc.identifierCONICET-
dc.identifier.urihttp://rodna.bn.gov.ar:8080/jspui/handle/bnmm/296033-
dc.descriptionAims: To investigate the potential to degrade polycyclic aromatic hydrocarbons (PAHs) of yet-to-be-cultured bacterial populations from chronically polluted intertidal sediments. Methods and Results: A gene variant encoding the alpha subunit of the catalytic component of an aromatic-ring-hydroxylating oxygenase (RHO) was abundant in intertidal sediments from chronically polluted subantarctic and temperate coastal environments, and its abundance increased after PAH amendment. Conversely, this marker gene was not detected in sediments from a nonimpacted site, even after a short-term PAH exposure. A metagenomic fragment carrying this gene variant was identified in a fosmid library of subantarctic sediments. This fragment contained five pairs of alpha and beta subunit genes and a lone alpha subunit gene of oxygenases, classified as belonging to three different RHO functional classes. In silico structural analysis suggested that two of these oxygenases contain large substrate-binding pockets, capable of accepting high molecular weight PAHs. Conclusions: The identified uncultured micro-organism presents the potential to degrade aromatic hydrocarbons with various chemical structures, and could represent an important member of the PAH-degrading community in these polluted coastal environments. Significance and Impact of the Study: This work provides valuable information for the design of environmental molecular diagnostic tools and for the biotechnological application of RHO enzymes.-
dc.descriptionFil: Loviso, Claudia Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; Argentina-
dc.descriptionFil: Lozada, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico; Argentina-
dc.descriptionFil: Guibert, Lilian Marcela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; Argentina-
dc.descriptionFil: Musumeci, Matias Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico; Argentina-
dc.descriptionFil: Sarango Cardenas, Sandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico; Argentina-
dc.descriptionFil: Kuin, Ruud V.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico; Argentina-
dc.descriptionFil: Marcos, Magalí Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico; Argentina-
dc.descriptionFil: Dionisi, Hebe Monica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto de Biología de Organismos Marinos; Argentina-
dc.formatapplication/pdf-
dc.formatapplication/pdf-
dc.formatapplication/pdf-
dc.languageeng-
dc.publisherWiley Blackwell Publishing, Inc-
dc.relationinfo:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/jam.12843/abstract-
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1111/jam.12843-
dc.rightsinfo:eu-repo/semantics/openAccess-
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/-
dc.sourcereponame:CONICET Digital (CONICET)-
dc.sourceinstname:Consejo Nacional de Investigaciones Científicas y Técnicas-
dc.sourceinstacron:CONICET-
dc.subjectINTERTIDAL SEDIMENTS-
dc.subjectMETAGENOMIC LIBRARY-
dc.subjectPOLYCYCLIC AROMATIC HYDROCARBONS-
dc.subjectPROTEIN MODELLING-
dc.subjectQUANTITATIVE PCR-
dc.subjectRING-HYDROXYLATING OXYGENASES-
dc.subjectBioremediación, Diagnóstico Biotecnológico en Gestión Medioambiental-
dc.subjectBiotecnología del Medio Ambiente-
dc.subjectINGENIERÍAS Y TECNOLOGÍAS-
dc.subjectBiología Celular, Microbiología-
dc.subjectCiencias Biológicas-
dc.subjectCIENCIAS NATURALES Y EXACTAS-
dc.titleMetagenomics reveals the high polycyclic aromatic hydrocarbon-degradation potential of abundant uncultured bacteria from chronically polluted subantarctic and temperate coastal marine environments-
dc.typeinfo:eu-repo/semantics/article-
dc.typeinfo:eu-repo/semantics/publishedVersion-
dc.typeinfo:ar-repo/semantics/articulo-
Aparece en las colecciones: CONICET

Ficheros en este ítem:
No hay ficheros asociados a este ítem.
Mostrar el registro sencillo del ítem