Registro completo de metadatos
| Campo DC | Valor | Lengua/Idioma |
|---|---|---|
| dc.creator | Asención Diez, Matías Damián | - |
| dc.creator | Miah, Farzana | - |
| dc.creator | Stevenson, Clare E. M. | - |
| dc.creator | Lawson, David M. | - |
| dc.creator | Iglesias, Alberto Alvaro | - |
| dc.creator | Bornemann, Stephen | - |
| dc.date | 2018-11-09T22:10:43Z | - |
| dc.date | 2018-11-09T22:10:43Z | - |
| dc.date | 2017-01 | - |
| dc.date | 2018-10-23T20:41:40Z | - |
| dc.date.accessioned | 2019-04-29T15:32:28Z | - |
| dc.date.available | 2019-04-29T15:32:28Z | - |
| dc.date.issued | 2018-11-09T22:10:43Z | - |
| dc.date.issued | 2018-11-09T22:10:43Z | - |
| dc.date.issued | 2017-01 | - |
| dc.date.issued | 2018-10-23T20:41:40Z | - |
| dc.identifier | Asención Diez, Matías Damián; Miah, Farzana; Stevenson, Clare E. M.; Lawson, David M.; Iglesias, Alberto Alvaro; et al.; The production and utilization of GDP-glucose in the biosynthesis of trehalose 6-phosphate by streptomyces venezuelae; American Society for Biochemistry and Molecular Biology; Journal of Biological Chemistry (online); 292; 3; 1-2017; 945-954 | - |
| dc.identifier | 0021-9258 | - |
| dc.identifier | http://hdl.handle.net/11336/64182 | - |
| dc.identifier | CONICET Digital | - |
| dc.identifier | CONICET | - |
| dc.identifier.uri | http://rodna.bn.gov.ar:8080/jspui/handle/bnmm/296091 | - |
| dc.description | Trehalose-6-phosphate synthase OtsA from streptomycetes is unusual in that it uses GDP-glucose as the donor substrate rather than the more commonly used UDP-glucose. We now confirm that OtsA from Streptomyces venezuelae has such a preference for GDP-glucose and can utilize ADP-glucose to some extent too. A crystal structure of the enzyme shows that it shares twin Rossmann-like domains with the UDP-glucose-specific OtsA from Escherichia coli. However, it is structurally more similar to Streptomyces hygroscopicus VldE, a GDP-valienol-dependent pseudoglycosyltransferase enzyme. Comparison of the donor binding sites reveals that the amino acids associated with the binding of diphosphoribose are almost all identical in these three enzymes. By contrast, the amino acids associated with binding guanine in VldE (Asn, Thr, and Val) are similar in S. venezuelae OtsA (Asp, Ser, and Phe, respectively) but not conserved in E. coli OtsA (His, Leu, and Asp, respectively), providing a rationale for the purine base specificity of S. venezuelae OtsA. To establish which donor is used in vivo, we generated an otsA null mutant in S. venezuelae. The mutant had a cell density-dependent growth phenotype and accumulated galactose 1-phosphate, glucose 1-phosphate, and GDP-glucose when grown on galactose. To determine how the GDP-glucose is generated, we characterized three candidate GDP-glucose pyrophosphorylases. SVEN-3027 is a UDP-glucose pyrophosphorylase, SVEN-3972 is an unusual ITP-mannose pyrophosphorylase, and SVEN-2781 is a pyrophosphorylase that is capable of generating GDP-glucose as well as GDP-mannose. We have therefore established how S. venezuelae can make and utilize GDP-glucose in the biosynthesis of trehalose 6-phosphate. | - |
| dc.description | Fil: Asención Diez, Matías Damián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina | - |
| dc.description | Fil: Miah, Farzana. John Innes Institute; Reino Unido | - |
| dc.description | Fil: Stevenson, Clare E. M.. John Innes Institute; Reino Unido | - |
| dc.description | Fil: Lawson, David M.. John Innes Institute; Reino Unido | - |
| dc.description | Fil: Iglesias, Alberto Alvaro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina | - |
| dc.description | Fil: Bornemann, Stephen. John Innes Institute; Reino Unido | - |
| dc.format | application/pdf | - |
| dc.format | application/vnd.openxmlformats-officedocument.wordprocessingml.document | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.language | eng | - |
| dc.publisher | American Society for Biochemistry and Molecular Biology | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1074/jbc.M116.758664 | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/url/http://www.jbc.org/content/292/3/945 | - |
| dc.rights | info:eu-repo/semantics/openAccess | - |
| dc.rights | https://creativecommons.org/licenses/by/2.5/ar/ | - |
| dc.source | reponame:CONICET Digital (CONICET) | - |
| dc.source | instname:Consejo Nacional de Investigaciones Científicas y Técnicas | - |
| dc.source | instacron:CONICET | - |
| dc.subject | trehalose | - |
| dc.subject | carbohydrate metabolism | - |
| dc.subject | glycosyltransferase | - |
| dc.subject | crystal structure | - |
| dc.subject | microbiology | - |
| dc.subject | substrate specificity | - |
| dc.subject | Otras Ciencias Biológicas | - |
| dc.subject | Ciencias Biológicas | - |
| dc.subject | CIENCIAS NATURALES Y EXACTAS | - |
| dc.title | The production and utilization of GDP-glucose in the biosynthesis of trehalose 6-phosphate by streptomyces venezuelae | - |
| dc.type | info:eu-repo/semantics/article | - |
| dc.type | info:eu-repo/semantics/publishedVersion | - |
| dc.type | info:ar-repo/semantics/articulo | - |
| Aparece en las colecciones: | CONICET | |
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