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dc.creatorLeal Denis, Maria Florencia-
dc.creatorIncicco, Juan Jeremías-
dc.creatorEspelt, Maria Victoria-
dc.creatorVerstraeten, Sandra Viviana-
dc.creatorPignataro, Omar Pedro-
dc.creatorLazarowski, Eduardo R.-
dc.creatorSchwarzbaum, Pablo Julio-
dc.date2015-11-12T19:24:37Z-
dc.date2015-11-12T19:24:37Z-
dc.date2013-10-
dc.date2016-03-30 10:35:44.97925-03-
dc.date.accessioned2019-04-29T15:32:32Z-
dc.date.available2019-04-29T15:32:32Z-
dc.date.issued2015-11-12T19:24:37Z-
dc.date.issued2015-11-12T19:24:37Z-
dc.date.issued2013-10-
dc.date.issued2016-03-30 10:35:44.97925-03-
dc.identifierLeal Denis, Maria Florencia; Incicco, Juan Jeremías; Espelt, Maria Victoria; Verstraeten, Sandra Viviana; Pignataro, Omar Pedro; et al.; Kinetics of extracellular ATP in mastoparan 7-activated human erythrocytes; Elsevier; Biochimica et Biophysica Acta- General Subjects; 1830; 10; 10-2013; 4692-4707-
dc.identifier0304-4165-
dc.identifierhttp://hdl.handle.net/11336/2772-
dc.identifier.urihttp://rodna.bn.gov.ar:8080/jspui/handle/bnmm/296120-
dc.descriptionBackground: The peptide mastoparan 7 (MST7) stimulated ATP release in human erythrocytes. We explored intraand extracellular processes governing the time-dependent accumulation of extracellular ATP (i.e., ATPe kinetics). Methods: Human erythrocytes were treated with MST7 in the presence or absence of two blockers of pannexin 1. ATPe concentration was monitored by luciferin–luciferase based real-time luminometry. Results: Exposure of human erythrocytes to MST7 led to an acute increase in [ATPe], followed by a slower increase phase. ATPe kinetics reflected a strong activation of ATP efflux and a low rate of ATPe hydrolysis by ectoATPase activity. Enhancement of [ATPe] by MST7 required adhesion of erythrocytes to poly-D-lysin-coated coverslips, and correlated with a 31% increase of cAMP and 10% cell swelling. However, when MST7 was dissolved in a hyperosmotic medium to block cell swelling, ATPe accumulation was inhibited by 49%. Erythrocytes pre-exposure to 10 μM of either carbenoxolone or probenecid, two blockers of pannexin 1, exhibited a partial reduction of ATP efflux. Erythrocytes from pannexin 1 knockout mice exhibited similar ATPe kinetics as those of wild type mice erythrocytes exposed to pannexin 1 blockers. Conclusions: MST7 induced release of ATP required either cell adhesion or strong activation of cAMP synthesis. Part of this release required cell swelling. Kinetic analysis and a data driven model suggested that ATP efflux is mediated by two ATP conduits displaying different kinetics, with one conduit being fully blocked by pannexin 1 blockers. General significance: Kinetic analysis of extracellular ATP accumulation from human erythrocytes and potential effects on microcirculation.-
dc.descriptionFil: Leal Denis, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Fisicoquímica Biológicas; Argentina-
dc.descriptionFil: Incicco, Juan Jeremías. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Fisicoquímica Biológicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina-
dc.descriptionFil: Espelt, Maria Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Fisicoquímica Biológicas; Argentina-
dc.descriptionFil: Verstraeten, Sandra Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Fisicoquímica Biológicas; Argentina-
dc.descriptionFil: Pignataro, Omar Pedro. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina-
dc.descriptionFil: Lazarowski, Eduardo R.. University of North Carolina at Chapel Hill. Cystic Fibrosis/Pulmonary Research and Treatment Center; Estados Unidos-
dc.descriptionFil: Schwarzbaum, Pablo Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Fisicoquímica Biológicas; Argentina-
dc.formatapplication/pdf-
dc.formatapplication/pdf-
dc.formatapplication/pdf-
dc.languageeng-
dc.publisherElsevier-
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.bbagen.2013.05.033-
dc.relationinfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0304416513002298-
dc.relationinfo:eu-repo/semantics/altIdentifier/issn/0304-4165-
dc.relationinfo:eu-repo/semantics/altIdentifier/url/http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3999873/-
dc.rightsinfo:eu-repo/semantics/openAccess-
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/-
dc.sourcereponame:CONICET Digital (CONICET)-
dc.sourceinstname:Consejo Nacional de Investigaciones Científicas y Técnicas-
dc.sourceinstacron:CONICET-
dc.subjectEXTRACELLULAR ATP-
dc.subjectATPASES-
dc.subjectERYTHROCYTE-
dc.subjectPANNEXIN 1-
dc.subjectBioquímica y Biología Molecular-
dc.subjectCiencias Biológicas-
dc.subjectCIENCIAS NATURALES Y EXACTAS-
dc.titleKinetics of extracellular ATP in mastoparan 7-activated human erythrocytes-
dc.typeinfo:eu-repo/semantics/article-
dc.typeinfo:eu-repo/semantics/publishedVersion-
dc.typeinfo:ar-repo/semantics/articulo-
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