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| Campo DC | Valor | Lengua/Idioma |
|---|---|---|
| dc.provenance | CONICET | - |
| dc.creator | Ferré, Luis Bernardo | - |
| dc.creator | Bogliotti, Yanina | - |
| dc.creator | Chitwood, James L. | - |
| dc.creator | Fresno Rodríguez, Cristóbal | - |
| dc.creator | Ortega, Hugo Hector | - |
| dc.creator | Kjelland, Michael E. | - |
| dc.creator | Ross, Pablo J. | - |
| dc.date | 2018-04-16T13:48:10Z | - |
| dc.date | 2018-04-16T13:48:10Z | - |
| dc.date | 2017-04 | - |
| dc.date | 2018-04-05T18:36:13Z | - |
| dc.date.accessioned | 2019-04-29T15:33:14Z | - |
| dc.date.available | 2019-04-29T15:33:14Z | - |
| dc.date.issued | 2017-04 | - |
| dc.identifier | Ferré, Luis Bernardo; Bogliotti, Yanina; Chitwood, James L.; Fresno Rodríguez, Cristóbal; Ortega, Hugo Hector; et al.; Effect of spermatozoa motility hyperactivation factors and gamete coincubation duration on in vitro bovine embryo development using flow cytometrically sorted spermatozoa; Csiro Publishing; Reproduction Fertility and Development; 29; 4; 4-2017; 805-814 | - |
| dc.identifier | 1031-3613 | - |
| dc.identifier | http://hdl.handle.net/11336/42094 | - |
| dc.identifier | CONICET Digital | - |
| dc.identifier | CONICET | - |
| dc.identifier.uri | http://rodna.bn.gov.ar:8080/jspui/handle/bnmm/296404 | - |
| dc.description | The aim of the present study was to evaluate the effects of sperm motility enhancers and different IVF times on cleavage, polyspermy, blastocyst formation, embryo quality and hatching ability. In Experiment 1, sex-sorted X chromosome-bearing Bos taurus spermatozoa were incubated for 30 min before 18 h fertilisation with hyperactivating factors, namely 10mM caffeine (CA), 5mMtheophylline (TH), 10mM caffeine and 5mMtheophylline (CA+TH); and untreated spermatozoa (control). In Experiment 2, matured B.Taurus oocytes were fertilised using a short (8 h) or standard (18 h) fertilisation length, comparing two different fertilisation media, namely synthetic oviducal fluid (SOF) fertilisation medium (SOF-FERT) and M199 fertilisation medium (M199-FERT). Cleavage and blastocyst formation rates were significantly higher in the CA+TH group (77% and 27%, respectively) compared with the control group (71% and 21%, respectively). Cleavage rates and blastocyst formation were significantly lower for the shortest fertilisation time (8 h) in M199-FERT medium (42% and 12%, respectively). The SOF-FERT medium with an 8 h fertilisation time resulted in the highest cleavage rates and blastocyst formation (74% and 29%, respectively). The SOF-FERT medium produced the highest embryo quality (50% Grade 1) and hatching rate (66%). Motility enhancers did not affect polyspermy rates, whereas polyspermy was affected when fertilisation length was extended from 8 h (3%) to 18 h (9%) and in M199-FERT (14%) compared with SOF-FERT (6%). We conclude that adding the motility enhancers CA and TH to sex sorted spermatozoa and Tyrode´s albumin lactate pyruvate (TALP)-Sperm can improve cleavage and embryo development rates without increasing polyspermy. In addition, shortening the oocyte-sperm coincubation time (8 h) resulted in similar overall embryo performance rates compared with the prolonged (18 h) interval. | - |
| dc.description | Fil: Ferré, Luis Bernardo. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina | - |
| dc.description | Fil: Bogliotti, Yanina. University of California; Estados Unidos | - |
| dc.description | Fil: Chitwood, James L.. University of California; Estados Unidos | - |
| dc.description | Fil: Fresno Rodríguez, Cristóbal. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Católica de Córdoba; Argentina | - |
| dc.description | Fil: Ortega, Hugo Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina | - |
| dc.description | Fil: Kjelland, Michael E.. Conservation, Genetics and Biotech; Estados Unidos | - |
| dc.description | Fil: Ross, Pablo J.. University of California; Estados Unidos | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.language | eng | - |
| dc.publisher | Csiro Publishing | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1071/RD15289 | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/url/http://www.publish.csiro.au/rd/RD15289 | - |
| dc.rights | info:eu-repo/semantics/restrictedAccess | - |
| dc.rights | https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ | - |
| dc.source | reponame:CONICET Digital (CONICET) | - |
| dc.source | instname:Consejo Nacional de Investigaciones Científicas y Técnicas | - |
| dc.source | instacron:CONICET | - |
| dc.source.uri | http://hdl.handle.net/11336/42094 | - |
| dc.subject | IVF | - |
| dc.subject | SEXED SPERMATOZOA | - |
| dc.subject | Biología Reproductiva | - |
| dc.subject | Ciencias Biológicas | - |
| dc.subject | CIENCIAS NATURALES Y EXACTAS | - |
| dc.title | Effect of spermatozoa motility hyperactivation factors and gamete coincubation duration on in vitro bovine embryo development using flow cytometrically sorted spermatozoa | - |
| dc.type | info:eu-repo/semantics/article | - |
| dc.type | info:eu-repo/semantics/publishedVersion | - |
| dc.type | info:ar-repo/semantics/articulo | - |
| Aparece en las colecciones: | CONICET | |
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