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| Campo DC | Valor | Lengua/Idioma |
|---|---|---|
| dc.provenance | CONICET | - |
| dc.creator | Nijhuis, Anke | - |
| dc.creator | Curciarello, Renata | - |
| dc.creator | Mehta, Shameer | - |
| dc.creator | Feakins, Roger | - |
| dc.creator | Bishop, Cleo L. | - |
| dc.creator | Lindsay, James O. | - |
| dc.creator | Silver, Andrew | - |
| dc.date | 2018-08-28T19:53:23Z | - |
| dc.date | 2018-08-28T19:53:23Z | - |
| dc.date | 2017-05 | - |
| dc.date | 2018-08-28T18:42:24Z | - |
| dc.date.accessioned | 2019-04-29T15:33:46Z | - |
| dc.date.available | 2019-04-29T15:33:46Z | - |
| dc.date.issued | 2017-05 | - |
| dc.identifier | Nijhuis, Anke; Curciarello, Renata; Mehta, Shameer; Feakins, Roger; Bishop, Cleo L.; et al.; MCL-1 is modulated in Crohn’s disease fibrosis by miR-29b via IL-6 and IL-8; Springer; Cell and Tissue Research; 368; 2; 5-2017; 325-335 | - |
| dc.identifier | 0302-766X | - |
| dc.identifier | http://hdl.handle.net/11336/57423 | - |
| dc.identifier | 1432-0878 | - |
| dc.identifier | CONICET Digital | - |
| dc.identifier | CONICET | - |
| dc.identifier.uri | http://rodna.bn.gov.ar:8080/jspui/handle/bnmm/296592 | - |
| dc.description | The miR-29 family is involved in fibrosis in multiple organs, including the intestine where miR-29b facilitates TGF-β-mediated up-regulation of collagen in mucosal fibroblasts from Crohn’s disease (CD) patients. Myeloid cell leukemia-1 (MCL-1), a member of the B-cell CLL/Lymphoma 2 (BCL-2) apoptosis family, is involved in liver fibrosis and is targeted by miR-29b via its 3’-UTR in cultured cell lines. We investigate the role of MCL-1 and miR-29b in primary intestinal fibroblasts and tissue from stricturing CD patients. Transfection of CD intestinal fibroblasts with pre-miR-29b resulted in a significant increase in the mRNA expression of MCL-1 isoforms [MCL-1Long (L)/Extra Short (ES) and MCL-1Short (S)], although MCL-1S was expressed at significantly lower levels. Western blotting predominantly detected the anti-apoptotic MCL-1L isoform, and immunofluorescence showed that staining was localised in discrete nuclear foci. Transfection with pre-miR-29b or anti-miR-29b resulted in a significant increase or decrease, respectively, in MCL-1L foci. CD fibroblasts treated with IL-6 and IL-8, inflammatory cytokines upstream of MCL-1, increased the total mass of MCL-1L-positive foci. Furthermore, transfection of intestinal fibroblasts with pre-miR-29b resulted in an increase in mRNA and protein levels of IL-6 and IL-8. Finally, immunohistochemistry showed reduced MCL-1 protein expression in fibrotic CD samples compared to non-stricturing controls. Together, our findings suggest that induction of MCL-1 by IL-6/IL-8 may surmount any direct down-regulation by miR-29b via its 3’-UTR. We propose that an anti-fibrotic miR-29b/IL-6 IL-8/MCL-1L axis may influence intestinal fibrosis in CD. In the future, therapeutic modulation of this pathway might contribute to the management of fibrosis in CD. | - |
| dc.description | Fil: Nijhuis, Anke. Queen Mary University of London; Reino Unido | - |
| dc.description | Fil: Curciarello, Renata. Queen Mary University of London; Reino Unido. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | - |
| dc.description | Fil: Mehta, Shameer. Queen Mary University of London; Reino Unido | - |
| dc.description | Fil: Feakins, Roger. The Royal London Hospital; Reino Unido | - |
| dc.description | Fil: Bishop, Cleo L.. Queen Mary University of London; Reino Unido | - |
| dc.description | Fil: Lindsay, James O.. Queen Mary University of London; Reino Unido | - |
| dc.description | Fil: Silver, Andrew. Queen Mary University of London; Reino Unido | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.language | eng | - |
| dc.publisher | Springer | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1007/s00441-017-2576-1 | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs00441-017-2576-1 | - |
| dc.rights | info:eu-repo/semantics/openAccess | - |
| dc.rights | https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ | - |
| dc.source | reponame:CONICET Digital (CONICET) | - |
| dc.source | instname:Consejo Nacional de Investigaciones Científicas y Técnicas | - |
| dc.source | instacron:CONICET | - |
| dc.source.uri | http://hdl.handle.net/11336/57423 | - |
| dc.subject | CROHN’S DISEASE | - |
| dc.subject | FIBROSIS | - |
| dc.subject | MCL-1 | - |
| dc.subject | MICRORNA | - |
| dc.subject | MIR-29B | - |
| dc.subject | Inmunología | - |
| dc.subject | Medicina Básica | - |
| dc.subject | CIENCIAS MÉDICAS Y DE LA SALUD | - |
| dc.title | MCL-1 is modulated in Crohn’s disease fibrosis by miR-29b via IL-6 and IL-8 | - |
| dc.type | info:eu-repo/semantics/article | - |
| dc.type | info:eu-repo/semantics/publishedVersion | - |
| dc.type | info:ar-repo/semantics/articulo | - |
| Aparece en las colecciones: | CONICET | |
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