Registro completo de metadatos
| Campo DC | Valor | Lengua/Idioma |
|---|---|---|
| dc.provenance | CONICET | - |
| dc.creator | Fathi, Fariba | - |
| dc.creator | Brun, Antonio | - |
| dc.creator | Rott, Katherine H. | - |
| dc.creator | Cobra, Paulo Falco | - |
| dc.creator | Tonelli, Marco | - |
| dc.creator | Eghbalnia, Hamid R. | - |
| dc.creator | Caviedes Vidal, Enrique Juan Raul | - |
| dc.creator | Karasov, William | - |
| dc.creator | Markley, John L. | - |
| dc.date | 2018-12-27T18:46:33Z | - |
| dc.date | 2018-12-27T18:46:33Z | - |
| dc.date | 2017-12 | - |
| dc.date | 2018-10-23T17:42:11Z | - |
| dc.date.accessioned | 2019-04-29T15:34:14Z | - |
| dc.date.available | 2019-04-29T15:34:14Z | - |
| dc.date.issued | 2017-12 | - |
| dc.identifier | Fathi, Fariba; Brun, Antonio; Rott, Katherine H.; Cobra, Paulo Falco; Tonelli, Marco; et al.; NMR-based identification of metabolites in polar and non-polar extracts of avian liver; MDPI; Metabolites; 7; 4; 12-2017; 1-9 | - |
| dc.identifier | 2218-1989 | - |
| dc.identifier | http://hdl.handle.net/11336/67096 | - |
| dc.identifier | CONICET Digital | - |
| dc.identifier | CONICET | - |
| dc.identifier.uri | http://rodna.bn.gov.ar:8080/jspui/handle/bnmm/296793 | - |
| dc.description | Metabolites present in liver provide important clues regarding the physiological state of an organism. The aim of this work was to evaluate a protocol for high-throughput NMR-based analysis of polar and non-polar metabolites from a small quantity of liver tissue. We extracted the tissue with a methanol/chloroform/water mixture and isolated the polar metabolites from the methanol/water layer and the non-polar metabolites from the chloroform layer. Following drying, we re-solubilized the fractions for analysis with a 600 MHz NMR spectrometer equipped with a 1.7 mm cryogenic probe. In order to evaluate the feasibility of this protocol for metabolomics studies, we analyzed the metabolic profile of livers from house sparrow (Passer domesticus) nestlings raised on two different diets: livers from 10 nestlings raised on a high protein diet (HP) for 4 d and livers from 12 nestlings raised on the HP diet for 3 d and then switched to a high carbohydrate diet (HC) for 1 d. The protocol enabled the detection of 52 polar and nine non-polar metabolites in 1H NMR spectra of the extracts. We analyzed the lipophilic metabolites by one-way ANOVA to assess statistically significant concentration differences between the two groups. The results of our studies demonstrate that the protocol described here can be exploited for high-throughput screening of small quantities of liver tissue (approx. 100 mg wet mass) obtainable from small animals. | - |
| dc.description | Fil: Fathi, Fariba. University of Wisconsin; Estados Unidos | - |
| dc.description | Fil: Brun, Antonio. University of Wisconsin; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | - |
| dc.description | Fil: Rott, Katherine H.. University of Wisconsin; Estados Unidos | - |
| dc.description | Fil: Cobra, Paulo Falco. University of Wisconsin; Estados Unidos | - |
| dc.description | Fil: Tonelli, Marco. University of Wisconsin; Estados Unidos | - |
| dc.description | Fil: Eghbalnia, Hamid R.. University of Wisconsin; Estados Unidos | - |
| dc.description | Fil: Caviedes Vidal, Enrique Juan Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina | - |
| dc.description | Fil: Karasov, William. University of Wisconsin; Estados Unidos | - |
| dc.description | Fil: Markley, John L.. University of Wisconsin; Estados Unidos | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.language | eng | - |
| dc.publisher | MDPI | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.3390/metabo7040061 | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2218-1989/7/4/61 | - |
| dc.rights | info:eu-repo/semantics/openAccess | - |
| dc.rights | https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ | - |
| dc.source | reponame:CONICET Digital (CONICET) | - |
| dc.source | instname:Consejo Nacional de Investigaciones Científicas y Técnicas | - |
| dc.source | instacron:CONICET | - |
| dc.source.uri | http://hdl.handle.net/11336/67096 | - |
| dc.subject | DIET | - |
| dc.subject | EFFECT OF ON LIVER METABOLITES | - |
| dc.subject | EXTRACTION PROTOCOL | - |
| dc.subject | LIVER TISSUE | - |
| dc.subject | METABOLITE IDENTIFICATION BY NMR | - |
| dc.subject | NMR SPECTROSCOPY | - |
| dc.subject | Otras Ciencias Químicas | - |
| dc.subject | Ciencias Químicas | - |
| dc.subject | CIENCIAS NATURALES Y EXACTAS | - |
| dc.title | NMR-based identification of metabolites in polar and non-polar extracts of avian liver | - |
| dc.type | info:eu-repo/semantics/article | - |
| dc.type | info:eu-repo/semantics/publishedVersion | - |
| dc.type | info:ar-repo/semantics/articulo | - |
| Aparece en las colecciones: | CONICET | |
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