Registro completo de metadatos
| Campo DC | Valor | Lengua/Idioma |
|---|---|---|
| dc.provenance | CONICET | - |
| dc.creator | Kraiselburd, Ivana | - |
| dc.creator | Gutt, Alexander | - |
| dc.creator | Losi, Aba | - |
| dc.creator | Gärtner, Wolfgang | - |
| dc.creator | Orellano, Elena Graciela | - |
| dc.date | 2018-07-23T17:23:15Z | - |
| dc.date | 2018-07-23T17:23:15Z | - |
| dc.date | 2015-09 | - |
| dc.date | 2018-07-20T18:03:02Z | - |
| dc.date.accessioned | 2019-04-29T15:34:36Z | - |
| dc.date.available | 2019-04-29T15:34:36Z | - |
| dc.date.issued | 2015-09 | - |
| dc.identifier | Kraiselburd, Ivana; Gutt, Alexander; Losi, Aba; Gärtner, Wolfgang; Orellano, Elena Graciela; Functional Characterization of a LOV-Histidine Kinase Photoreceptor from Xanthomonas citri subsp. citri; Wiley Blackwell Publishing, Inc; Photochemistry and Photobiology; 91; 5; 9-2015; 1123-1132 | - |
| dc.identifier | 0031-8655 | - |
| dc.identifier | http://hdl.handle.net/11336/52828 | - |
| dc.identifier | CONICET Digital | - |
| dc.identifier | CONICET | - |
| dc.identifier.uri | http://rodna.bn.gov.ar:8080/jspui/handle/bnmm/296951 | - |
| dc.description | The blue-light (BL) absorbing protein Xcc-LOV from Xanthomonas citri subsp. citri is composed of a LOV-domain, a histidine kinase (HK) and a response regulator. Spectroscopic characterization of Xcc-LOV identified intermediates and kinetics of the protein's photocycle. Measurements of steady state and time-resolved fluorescence allowed determination of quantum yields for triplet (ΦT = 0.68 ± 0.03) and photoproduct formation (Φ390 = 0.46 ± 0.05). The lifetime for triplet decay was determined as τT = 2.4-2.8 μs. Fluorescence of tryptophan and tyrosine residues was unchanged upon light-to-dark conversion, emphasizing the absence of significant conformational changes. Photochemistry was blocked upon cysteine C76 (C76S) mutation, causing a seven-fold longer lifetime of the triplet state (τT = 16-18.5 μs). Optoacoustic spectroscopy yielded the energy content of the triplet state. Interestingly, Xcc-LOV did not undergo the volume contraction reported for other LOV domains within the observation time window, although the back-conversion into the dark state was accompanied by a volume expansion. A radioactivity-based enzyme function assay revealed a larger HK activity in the lit than in the dark state. The C76S mutant showed a still lower enzyme function, indicating the dark state activity being corrupted by a remaining portion of the long-lived lit state. | - |
| dc.description | Fil: Kraiselburd, Ivana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina | - |
| dc.description | Fil: Gutt, Alexander. Max‐Planck‐Institute for Chemical Energy Conversion; Alemania | - |
| dc.description | Fil: Losi, Aba. Università di Parma; Italia | - |
| dc.description | Fil: Gärtner, Wolfgang. Max‐Planck‐Institute for Chemical Energy Conversion; Alemania | - |
| dc.description | Fil: Orellano, Elena Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.language | eng | - |
| dc.publisher | Wiley Blackwell Publishing, Inc | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1111/php.12493 | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/php.12493 | - |
| dc.rights | info:eu-repo/semantics/restrictedAccess | - |
| dc.rights | https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ | - |
| dc.source | reponame:CONICET Digital (CONICET) | - |
| dc.source | instname:Consejo Nacional de Investigaciones Científicas y Técnicas | - |
| dc.source | instacron:CONICET | - |
| dc.source.uri | http://hdl.handle.net/11336/52828 | - |
| dc.subject | LOV Protein | - |
| dc.subject | Histidine Kinase | - |
| dc.subject | Flash Photolysis | - |
| dc.subject | Optoacustics | - |
| dc.subject | Otras Ciencias Biológicas | - |
| dc.subject | Ciencias Biológicas | - |
| dc.subject | CIENCIAS NATURALES Y EXACTAS | - |
| dc.title | Functional Characterization of a LOV-Histidine Kinase Photoreceptor from Xanthomonas citri subsp. citri | - |
| dc.type | info:eu-repo/semantics/article | - |
| dc.type | info:eu-repo/semantics/publishedVersion | - |
| dc.type | info:ar-repo/semantics/articulo | - |
| Aparece en las colecciones: | CONICET | |
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