Registro completo de metadatos
| Campo DC | Valor | Lengua/Idioma |
|---|---|---|
| dc.provenance | CONICET | - |
| dc.creator | Wilda, Maximiliano | - |
| dc.creator | Lavoria, Maria de Los Angeles | - |
| dc.creator | Giraldez, Adrian Nicolas | - |
| dc.creator | Franco Mahecha, Olga Lucia | - |
| dc.creator | Mansilla, Florencia Celeste | - |
| dc.creator | Érguiz, Matías | - |
| dc.creator | Iglesias, Marcela Elvira | - |
| dc.creator | Capozzo, Alejandra Victoria | - |
| dc.date | 2019-01-21T23:19:07Z | - |
| dc.date | 2019-01-21T23:19:07Z | - |
| dc.date | 2012-11 | - |
| dc.date | 2019-01-09T17:50:47Z | - |
| dc.date.accessioned | 2019-04-29T15:34:38Z | - |
| dc.date.available | 2019-04-29T15:34:38Z | - |
| dc.date.issued | 2012-11 | - |
| dc.identifier | Wilda, Maximiliano; Lavoria, Maria de Los Angeles; Giraldez, Adrian Nicolas; Franco Mahecha, Olga Lucia; Mansilla, Florencia Celeste; et al.; Development and preliminary validation of an antibody filtration-assisted single-dilution chemiluminometric immunoassay for potency testing of Piscirickettsia salmonis vaccines; Academic Press Ltd - Elsevier Science Ltd; Biologicals; 40; 6; 11-2012; 415-420 | - |
| dc.identifier | 1045-1056 | - |
| dc.identifier | http://hdl.handle.net/11336/68356 | - |
| dc.identifier | CONICET Digital | - |
| dc.identifier | CONICET | - |
| dc.identifier.uri | http://rodna.bn.gov.ar:8080/jspui/handle/bnmm/296969 | - |
| dc.description | Challenge with live pathogens could be substituted by serology for many veterinary diseases, however little progress has been made in the development of alternative batch vaccine potency tests for fish. This study reports the development and preliminary validation of a single-dilution filtration-assisted chemiluminometric immunoassay (SD FAL-ELISA) applied to measure anti Piscirickettsia salmonis IgM in individual or pooled serum and mucus samples. The assay was set up to test a single-dilution of the sample. Serum SD FAL-ELISA yielded a sensitivity of 90% and a specificity of 96%. SD FAL-ELISA was applied to evaluate pooled and individual samples from P. salmonis challenge assessments. Relative-light units values (RLU) obtained by SD FAL-ELISA were proportional to antibody levels in serum. RLU values obtained from pooled and individual serum samples increased with the observed relative percent survival (RPS) values, indicating a correlation between protection and specific IgM levels. Results obtained for specific IgM in mucus samples was not related to the RPS, but discriminated the vaccine that yielded high RPS (86.4%) from the others (40.9 and 54.5%). This is the first report on the development of an indirect high-throughput serological assessment for P. salmonis vaccine potency testing using both pooled or individual serum and cutaneous mucus samples. | - |
| dc.description | Fil: Wilda, Maximiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencia y Tecnología "Dr. César Milstein". Fundación Pablo Cassará. Instituto de Ciencia y Tecnología ; Argentina | - |
| dc.description | Fil: Lavoria, Maria de Los Angeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencia y Tecnología "Dr. César Milstein". Fundación Pablo Cassará. Instituto de Ciencia y Tecnología ; Argentina | - |
| dc.description | Fil: Giraldez, Adrian Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencia y Tecnología "Dr. César Milstein". Fundación Pablo Cassará. Instituto de Ciencia y Tecnología ; Argentina | - |
| dc.description | Fil: Franco Mahecha, Olga Lucia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria; Argentina | - |
| dc.description | Fil: Mansilla, Florencia Celeste. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria; Argentina | - |
| dc.description | Fil: Érguiz, Matías. Tecnovax S.A.; Argentina | - |
| dc.description | Fil: Iglesias, Marcela Elvira. Tecnovax S.A.; Argentina | - |
| dc.description | Fil: Capozzo, Alejandra Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.language | eng | - |
| dc.publisher | Academic Press Ltd - Elsevier Science Ltd | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1016/j.biologicals.2012.09.003 | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1045105612001443 | - |
| dc.rights | info:eu-repo/semantics/restrictedAccess | - |
| dc.rights | https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ | - |
| dc.source | reponame:CONICET Digital (CONICET) | - |
| dc.source | instname:Consejo Nacional de Investigaciones Científicas y Técnicas | - |
| dc.source | instacron:CONICET | - |
| dc.source.uri | http://hdl.handle.net/11336/68356 | - |
| dc.subject | FAL-ELISA | - |
| dc.subject | PISCIRICKETTSIA SALMONIS | - |
| dc.subject | REFINEMENT | - |
| dc.subject | SEROLOGY | - |
| dc.subject | VACCINE POTENCY | - |
| dc.subject | Otras Ciencias Biológicas | - |
| dc.subject | Ciencias Biológicas | - |
| dc.subject | CIENCIAS NATURALES Y EXACTAS | - |
| dc.title | Development and preliminary validation of an antibody filtration-assisted single-dilution chemiluminometric immunoassay for potency testing of Piscirickettsia salmonis vaccines | - |
| dc.type | info:eu-repo/semantics/article | - |
| dc.type | info:eu-repo/semantics/publishedVersion | - |
| dc.type | info:ar-repo/semantics/articulo | - |
| Aparece en las colecciones: | CONICET | |
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