Registro completo de metadatos
| Campo DC | Valor | Lengua/Idioma |
|---|---|---|
| dc.creator | Bonazza, Horacio | - |
| dc.creator | Manzo, Ricardo Martín | - |
| dc.creator | Dos Santos, José C.S. | - |
| dc.creator | Mammarella, Enrique José | - |
| dc.date | 2018-01-16T17:35:17Z | - |
| dc.date | 2018-01-16T17:35:17Z | - |
| dc.date | 2017-06 | - |
| dc.date | 2018-01-12T19:44:31Z | - |
| dc.date.accessioned | 2019-04-29T15:34:59Z | - |
| dc.date.available | 2019-04-29T15:34:59Z | - |
| dc.date.issued | 2018-01-16T17:35:17Z | - |
| dc.date.issued | 2018-01-16T17:35:17Z | - |
| dc.date.issued | 2017-06 | - |
| dc.date.issued | 2018-01-12T19:44:31Z | - |
| dc.identifier | Dos Santos, José C.S.; Mammarella, Enrique José; Bonazza, Horacio; Manzo, Ricardo Martín; Operational and Thermal Stability Analysis of Thermomyces lanuginosus Lipase Covalently Immobilized onto Modified Chitosan Supports; Humana Press; Applied Biochemistry And Biotechnology; 184; 6-2017; 182-196 | - |
| dc.identifier | 0273-2289 | - |
| dc.identifier | http://hdl.handle.net/11336/33425 | - |
| dc.identifier | CONICET Digital | - |
| dc.identifier | CONICET | - |
| dc.identifier.uri | http://rodna.bn.gov.ar:8080/jspui/handle/bnmm/297135 | - |
| dc.description | The aim of this paper was to evaluate different strategies of chitosan activation using cross-linking reagent like glycidol, epichlorohydrin, and glutaraldehyde for Thermomyces lanuginosus lipase (TLL) immobilization. Operational activity and stability by esterification of oleic acid with ethanol and thermal inactivation using these derivatives were investigated. Derivative obtained by sequentially activation with glycidol, ethylenediamine, and glutaraldehyde and subsequent TLL immobilization showed the best performance, with high hydrolytic activity value. Its stability was 15-fold higher than solubilized TLL in the evaluated inactivation conditions (60 °C, 25 mM sodium phosphate buffer pH 7). After 5 cycles of oleic acid esterification, only a few percentage of its conversion has reduced. On the other hand, glycidol-activated chitosan derivative showed very low hydrolytic activity value. Epichlorohydrin-activated chitosan derivative showed regular hydrolytic activity value. Both derivatives showed low immobilization yields. Operational stability of this last derivative was very low, where after the first cycle of oleic acid esterification, only 56% of its initial conversion was obtained. | - |
| dc.description | Fil: Bonazza, Horacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina | - |
| dc.description | Fil: Manzo, Ricardo Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina | - |
| dc.description | Fil: Dos Santos, José C.S.. Universidade Da Integração Internacional Da Lusofonia Afro-brasileira; | - |
| dc.description | Fil: Mammarella, Enrique José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.language | eng | - |
| dc.publisher | Humana Press | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1007/s12010-017-2546-9 | - |
| dc.rights | info:eu-repo/semantics/restrictedAccess | - |
| dc.rights | https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ | - |
| dc.source | reponame:CONICET Digital (CONICET) | - |
| dc.source | instname:Consejo Nacional de Investigaciones Científicas y Técnicas | - |
| dc.source | instacron:CONICET | - |
| dc.subject | BIODIESEL | - |
| dc.subject | CHITOSAN | - |
| dc.subject | ESTERIFICATION | - |
| dc.subject | IMMOBILIZATION | - |
| dc.subject | LIPASE | - |
| dc.subject | THERMOMYCES LANUGINOSUS | - |
| dc.subject | Biotecnología Industrial | - |
| dc.subject | Biotecnología Industrial | - |
| dc.subject | INGENIERÍAS Y TECNOLOGÍAS | - |
| dc.title | Operational and Thermal Stability Analysis of Thermomyces lanuginosus Lipase Covalently Immobilized onto Modified Chitosan Supports | - |
| dc.type | info:eu-repo/semantics/article | - |
| dc.type | info:eu-repo/semantics/publishedVersion | - |
| dc.type | info:ar-repo/semantics/articulo | - |
| Aparece en las colecciones: | CONICET | |
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