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dc.creatorMarfil, Carlos Federico-
dc.creatorAsurmendi, Sebastian-
dc.creatorMasuelli, Ricardo Williams-
dc.date2018-04-18T14:05:21Z-
dc.date2018-04-18T14:05:21Z-
dc.date2012-04-08-
dc.date2018-04-05T18:18:11Z-
dc.date.accessioned2019-04-29T15:35:17Z-
dc.date.available2019-04-29T15:35:17Z-
dc.date.issued2018-04-18T14:05:21Z-
dc.date.issued2018-04-18T14:05:21Z-
dc.date.issued2012-04-08-
dc.date.issued2018-04-05T18:18:11Z-
dc.identifierMarfil, Carlos Federico; Asurmendi, Sebastian; Masuelli, Ricardo Williams; Changes in micro RNA expression in a wild tuber-bearing Solanum species induced by 5-Azacytidine treatment; Springer; Plant Cell Reports; 31; 8; 8-4-2012; 1449-1461-
dc.identifier0721-7714-
dc.identifierhttp://hdl.handle.net/11336/42429-
dc.identifier1432-203X-
dc.identifierCONICET Digital-
dc.identifierCONICET-
dc.identifier.urihttp://rodna.bn.gov.ar:8080/jspui/handle/bnmm/297255-
dc.descriptionPhenotypic plasticity is often postulated as a principal characteristic of tuber-bearing wild Solanum species. The hypotheses to explore this observation have been developed based on the presence of genetic variation. In this context, evolutionary changes and adaptation are impossible without genetic variation. However, epigenetic effects, which include DNA methylation and microRNAs expression control, could be another source of phenotypic variation in ecologically relevant traits. To achieve a detailed mechanistic understanding of these processes, it is necessary to separate epigenetic from DNA sequencebased effects and to evaluate their relative importance on phenotypic variability. We explored the potential relevance of epigenetic effects in individuals with the same genotype. For this purpose, a clone of the wild potato Solanum ruizlealii, a non-model species in which natural methylation variability has been demonstrated, was selected and its DNA methylation was manipulated applying 5-Azacytidine (AzaC), a demethylating agent. The AzaC treatment induced early flowering and changes in leaf morphology. Using quantitative real-time PCR, we identified four miRNAs up-regulated in the AzaC-treated plants. One of them, miRNA172, could play a role on the early flowering phenotype. In this work, we showed that the treatment with AzaC could provide meaningful results allowing to study both the phenotypic plasticity in tuber-bearing Solanum species and the inter-relation between DNA methylation and miRNA accumulations in a wide range of species.-
dc.descriptionFil: Marfil, Carlos Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina-
dc.descriptionFil: Asurmendi, Sebastian. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina-
dc.descriptionFil: Masuelli, Ricardo Williams. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina. Instituto Nacional de Tecnología Agropecuaria; Argentina-
dc.formatapplication/pdf-
dc.formatapplication/pdf-
dc.languageeng-
dc.publisherSpringer-
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1007/s00299-012-1260-x-
dc.relationinfo:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs00299-012-1260-x-
dc.rightsinfo:eu-repo/semantics/restrictedAccess-
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/-
dc.sourcereponame:CONICET Digital (CONICET)-
dc.sourceinstname:Consejo Nacional de Investigaciones Científicas y Técnicas-
dc.sourceinstacron:CONICET-
dc.subjectDNA METHYLATION-
dc.subjectEPIGENETICS-
dc.subjectMICRO RNAs-
dc.subjectPHENOTYPIC PLASTICITY-
dc.subjectWILD POTATO SPECIES-
dc.subjectOtras Ciencias Biológicas-
dc.subjectCiencias Biológicas-
dc.subjectCIENCIAS NATURALES Y EXACTAS-
dc.titleChanges in micro RNA expression in a wild tuber-bearing Solanum species induced by 5-Azacytidine treatment-
dc.typeinfo:eu-repo/semantics/article-
dc.typeinfo:eu-repo/semantics/publishedVersion-
dc.typeinfo:ar-repo/semantics/articulo-
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