Registro completo de metadatos
Campo DC Valor Lengua/Idioma
dc.creatorLaino, Aldana-
dc.creatorCunningham, Monica Liliana-
dc.creatorSuarez, Gustavo-
dc.creatorGarcia, Carlos Fernando-
dc.date2018-06-18T17:09:45Z-
dc.date2018-06-18T17:09:45Z-
dc.date2015-01-
dc.date2018-06-14T19:03:51Z-
dc.date.accessioned2019-04-29T15:35:28Z-
dc.date.available2019-04-29T15:35:28Z-
dc.date.issued2018-06-18T17:09:45Z-
dc.date.issued2018-06-18T17:09:45Z-
dc.date.issued2015-01-
dc.date.issued2018-06-14T19:03:51Z-
dc.identifierLaino, Aldana; Cunningham, Monica Liliana; Suarez, Gustavo; Garcia, Carlos Fernando; Identification and Characterization of the Lipid Transport System in the Tarantula Grammostola rosea; Scientific Research Publishing; Open Journal of Animal Sciences; 5; 1; 1-2015; 9-20-
dc.identifier2161-7597-
dc.identifierhttp://hdl.handle.net/11336/49010-
dc.identifierCONICET Digital-
dc.identifierCONICET-
dc.identifier.urihttp://rodna.bn.gov.ar:8080/jspui/handle/bnmm/297325-
dc.descriptionLipids due to its hydrophobic nature are transported in the hemolymph mainly by lipoproteic fractions. In the present work we studied the lipoproteic fractions present in the hemolymph of the spider Grammostola rosea. Through ultracentrifugation two lipoproteic fractions are isolated, one of high density named Gr-HDL (δ = 1.13 - 1.15 g/ml) and the other of very high density called Gr-VHDL (δ = 1.24 - 1.27 g/ml) Gr-VHDL in hemolymph is majority in relation to Gr-HDL. In this sense Gr-VHDL fraction has 98.6% of hemolymphatic proteins, and 89.3% of lipids presents in the hemolymph. Both lipoproteic fractions possess phospholipids such as majority lipids (phosphatidylcholine and phosphatidylethanolamine) and 18:1, 16:0, 18:2 and 18:0 as the major fatty acids. In order to confirm the role played by lipoproteic fractions in vitro assays with different 14C-lipid were performed. It was observed that Gr-VHDL takes up mainly free fatty acids and triacylglycerols unlike that observed for Gr-HDL in relation to phosphatidylcholine. Through electrophoresis it was observed that Gr-VHDL has three proteins: a predominant band of 68 kDa and two others of 99 and 121 kDa. Gr-HDL displayed a predominant band of 93 kDa, and other minority of 249 kDa. In conclusion, this study reports lipid characterization of the lipoproteic fractions present in the hemolymph of the tarantula, G. rosea. The role of each lipoproteic fraction in relation to lipid up- take is sustained by in vitro assays. Similarities and differences are found when it is compared to lipoproteins of only the three species of spiders studied.-
dc.descriptionFil: Laino, Aldana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata ; Argentina-
dc.descriptionFil: Cunningham, Monica Liliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata ; Argentina-
dc.descriptionFil: Suarez, Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata ; Argentina-
dc.descriptionFil: Garcia, Carlos Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata ; Argentina-
dc.formatapplication/pdf-
dc.formatapplication/pdf-
dc.languageeng-
dc.publisherScientific Research Publishing-
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.4236/ojas.2015.51002-
dc.relationinfo:eu-repo/semantics/altIdentifier/url/http://file.scirp.org/Html/2-1400219_52876.htm-
dc.rightsinfo:eu-repo/semantics/openAccess-
dc.rightshttps://creativecommons.org/licenses/by/2.5/ar/-
dc.sourcereponame:CONICET Digital (CONICET)-
dc.sourceinstname:Consejo Nacional de Investigaciones Científicas y Técnicas-
dc.sourceinstacron:CONICET-
dc.subjectSPIDER-
dc.subjectLIPOPROTEINS-
dc.subjectHEMOCYANIN-
dc.subjectLIPIDS-
dc.subjectARACHNIDS-
dc.subjectOtras Ciencias Biológicas-
dc.subjectCiencias Biológicas-
dc.subjectCIENCIAS NATURALES Y EXACTAS-
dc.titleIdentification and Characterization of the Lipid Transport System in the Tarantula Grammostola rosea-
dc.typeinfo:eu-repo/semantics/article-
dc.typeinfo:eu-repo/semantics/publishedVersion-
dc.typeinfo:ar-repo/semantics/articulo-
Aparece en las colecciones: CONICET

Ficheros en este ítem:
No hay ficheros asociados a este ítem.