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Campo DC Valor Lengua/Idioma
dc.creatorBarrio, Alejandra-
dc.creatorMora, Maria Celia-
dc.creatorRamos, Federico-
dc.creatorMoreno, Sonia-
dc.creatorSamson, Ruth-
dc.creatorBasombrio, Miguel Angel Manuel-
dc.date2017-12-07T18:29:15Z-
dc.date2017-12-07T18:29:15Z-
dc.date2007-10-
dc.date2017-05-29T15:38:21Z-
dc.date.accessioned2019-04-29T15:35:32Z-
dc.date.available2019-04-29T15:35:32Z-
dc.date.issued2017-12-07T18:29:15Z-
dc.date.issued2017-12-07T18:29:15Z-
dc.date.issued2007-10-
dc.date.issued2017-05-29T15:38:21Z-
dc.identifierBarrio, Alejandra; Mora, Maria Celia; Ramos, Federico; Moreno, Sonia; Samson, Ruth; et al.; Use of kDNA-based Polymerase Chain Reaction as a Sensitive and Differentially Diagnostic Method of American Tegumentary Leishmaniasis in Disease-Endemic Areas of Northern Argentina; American Society of Tropical Medicine and Hygiene; American Journal of Tropical Medicine and Hygiene; 77; 4; 10-2007; 636-639-
dc.identifier0002-9637-
dc.identifierhttp://hdl.handle.net/11336/29990-
dc.identifier1476-1645-
dc.identifierCONICET Digital-
dc.identifierCONICET-
dc.identifier.urihttp://rodna.bn.gov.ar:8080/jspui/handle/bnmm/297342-
dc.descriptionWe evaluated the polymerase chain reaction (PCR) for the diagnosis of endemic American Tegumentary Leishmaniasis in Salta, Argentina. Diverse Leishmania species, coexistence of mycotic and varicose ulcers, and high endemicity T. cruzi, represent diagnostic challenges in the region. We performed a simplified PCR using sensitive, generic primers on samples obtained by a non-invasive method. We tested different culture types and clinical specimens with other microorganisms that induce leishmaniasis-like lesions. The PCR had a sensitivity and specificity of 100%. Forty-five patients with presumptive leishmaniasis were compared to the PCR, smears, and the Montenegro skin test (MST). In the same population, the PCR had an increased sensitivity, detecting 25 of 45 cases compared with 16 of 45 for smears and had a higher sensitivity in detecting mucocutaneous lesions. Diagnosis by PCR was supported by clinical presentation, positive MST results, compatible epidemiology, and in some cases histopathologic results or isolation of parasites by culture. These findings indicate the convenience of incorporating this PCR into diagnostic strategies for detecting leishmaniasis in northern Argentina.-
dc.descriptionFil: Barrio, Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina-
dc.descriptionFil: Mora, Maria Celia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina-
dc.descriptionFil: Ramos, Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina-
dc.descriptionFil: Moreno, Sonia. Provincia de Salta. Ministerio de Salud Publica. Hospital del Milagro; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina-
dc.descriptionFil: Samson, Ruth. Provincia de Salta. Ministerio de Salud Pública. Hospital del Milagro; Argentina-
dc.descriptionFil: Basombrio, Miguel Angel Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina-
dc.formatapplication/pdf-
dc.formatapplication/pdf-
dc.languageeng-
dc.publisherAmerican Society of Tropical Medicine and Hygiene-
dc.relationinfo:eu-repo/semantics/reference/url/http://www.ajtmh.org/content/journals/10.4269/ajtmh.2007.77.636-
dc.relationinfo:eu-repo/semantics/altIdentifier/url/http://www.ajtmh.org/content/journals/10.4269/ajtmh.2007.77.636-
dc.rightsinfo:eu-repo/semantics/restrictedAccess-
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/-
dc.sourcereponame:CONICET Digital (CONICET)-
dc.sourceinstname:Consejo Nacional de Investigaciones Científicas y Técnicas-
dc.sourceinstacron:CONICET-
dc.subjectPolymerase Chain Reaction-
dc.subjectLeishmaniasis American-
dc.subjectk DNA-
dc.subjectDiagnostic-
dc.subjectBiología Celular, Microbiología-
dc.subjectCiencias Biológicas-
dc.subjectCIENCIAS NATURALES Y EXACTAS-
dc.titleUse of kDNA-based Polymerase Chain Reaction as a Sensitive and Differentially Diagnostic Method of American Tegumentary Leishmaniasis in Disease-Endemic Areas of Northern Argentina-
dc.typeinfo:eu-repo/semantics/article-
dc.typeinfo:eu-repo/semantics/publishedVersion-
dc.typeinfo:ar-repo/semantics/articulo-
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