Registro completo de metadatos
| Campo DC | Valor | Lengua/Idioma |
|---|---|---|
| dc.provenance | CONICET | - |
| dc.creator | Valverde, Carlos Alfredo | - |
| dc.creator | Kornyeyev, Dmytro | - |
| dc.creator | Ferreiro, Marcela | - |
| dc.creator | Petrosky, Azadé D. | - |
| dc.creator | Mattiazzi, Ramona Alicia | - |
| dc.creator | Escobar, Ariel Luis Manuel | - |
| dc.date | 2018-10-09T16:34:07Z | - |
| dc.date | 2018-10-09T16:34:07Z | - |
| dc.date | 2010-03 | - |
| dc.date | 2018-09-10T16:44:24Z | - |
| dc.date.accessioned | 2019-04-29T15:36:10Z | - |
| dc.date.available | 2019-04-29T15:36:10Z | - |
| dc.date.issued | 2010-03 | - |
| dc.identifier | Valverde, Carlos Alfredo; Kornyeyev, Dmytro; Ferreiro, Marcela; Petrosky, Azadé D.; Mattiazzi, Ramona Alicia; et al.; Transient Ca2+ depletion of the sarcoplasmic reticulum at the onset of reperfusion; Oxford University Press; Cardiovascular Research; 85; 4; 3-2010; 671-680 | - |
| dc.identifier | 0008-6363 | - |
| dc.identifier | http://hdl.handle.net/11336/61958 | - |
| dc.identifier | CONICET Digital | - |
| dc.identifier | CONICET | - |
| dc.identifier.uri | http://rodna.bn.gov.ar:8080/jspui/handle/bnmm/297508 | - |
| dc.description | AimsMyocardial stunning is a contractile dysfunction that occurs after a brief ischaemic insult. Substantial evidence supports that this dysfunction is triggered by Ca2+ overload during reperfusion. The aim of the present manuscript is to define the origin of this Ca2+ increase in the intact heart.Methods and resultsTo address this issue, Langendorff-perfused mouse hearts positioned on a pulsed local field fluorescence microscope and loaded with fluorescent dyes Rhod-2, Mag-fluo-4, and Di-8-ANEPPS, to assess cytosolic Ca2+, sarcoplasmic reticulum (SR) Ca2+, and transmembrane action potentials (AP), respectively, in the epicardial layer of the hearts, were submitted to 12 min of global ischaemia followed by reperfusion. Ischaemia increased cytosolic Ca2+ in association with a decrease in intracellular Ca2+ transients and a depression of Ca2+ transient kinetics, i.e. the rise time and decay time constant of Ca2+ transients were significantly prolonged. Reperfusion produced a transient increase in cytosolic Ca2+ (Ca2+ bump), which was temporally associated with a decrease in SR-Ca2+ content, as a mirror-like image. Caffeine pulses (20 mM) confirmed that SR-Ca2+ content was greatly diminished at the onset of reflow. The SR-Ca2+ decrease was associated with a decrease in Ca2+ transient amplitude and a shortening of AP duration mainly due to a decrease in phase 2.ConclusionTo the best of our knowledge, this is the first study in which SR-Ca2+ transients are recorded in the intact heart, revealing a previously unknown participation of SR on cytosolic Ca2+ overload upon reperfusion in the intact beating heart. Additionally, the associated shortening of phase 2 of the AP may provide a clue to explain early reperfusion arrhythmias. | - |
| dc.description | Fil: Valverde, Carlos Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones Cardiovasculares "Dr. Horacio Eugenio Cingolani". Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Investigaciones Cardiovasculares "Dr. Horacio Eugenio Cingolani"; Argentina | - |
| dc.description | Fil: Kornyeyev, Dmytro. University of California; Estados Unidos | - |
| dc.description | Fil: Ferreiro, Marcela. University of California; Estados Unidos | - |
| dc.description | Fil: Petrosky, Azadé D.. University of California; Estados Unidos | - |
| dc.description | Fil: Mattiazzi, Ramona Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones Cardiovasculares "Dr. Horacio Eugenio Cingolani". Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Investigaciones Cardiovasculares "Dr. Horacio Eugenio Cingolani"; Argentina | - |
| dc.description | Fil: Escobar, Ariel Luis Manuel. University of California; Estados Unidos | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.language | eng | - |
| dc.publisher | Oxford University Press | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1093/cvr/cvp371 | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/cardiovascres/article/85/4/671/297526 | - |
| dc.rights | info:eu-repo/semantics/openAccess | - |
| dc.rights | https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ | - |
| dc.source | reponame:CONICET Digital (CONICET) | - |
| dc.source | instname:Consejo Nacional de Investigaciones Científicas y Técnicas | - |
| dc.source | instacron:CONICET | - |
| dc.source.uri | http://hdl.handle.net/11336/61958 | - |
| dc.subject | CALCIUM | - |
| dc.subject | FLUORESCENCE | - |
| dc.subject | ISCHAEMIA/REPERFUSION | - |
| dc.subject | SARCOPLASMIC RETICULUM | - |
| dc.subject | Inmunología | - |
| dc.subject | Medicina Básica | - |
| dc.subject | CIENCIAS MÉDICAS Y DE LA SALUD | - |
| dc.title | Transient Ca2+ depletion of the sarcoplasmic reticulum at the onset of reperfusion | - |
| dc.type | info:eu-repo/semantics/article | - |
| dc.type | info:eu-repo/semantics/publishedVersion | - |
| dc.type | info:ar-repo/semantics/articulo | - |
| Aparece en las colecciones: | CONICET | |
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