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dc.provenanceCONICET-
dc.creatorSingh, Naveen Kumar-
dc.creatorDsouza, Roy N.-
dc.creatorGrasselli, Mariano-
dc.creatorFernández Lahore, Marcelo-
dc.date2017-12-22T21:11:46Z-
dc.date2017-12-22T21:11:46Z-
dc.date2014-06-
dc.date2017-12-21T19:58:40Z-
dc.date.accessioned2019-04-29T15:36:26Z-
dc.date.available2019-04-29T15:36:26Z-
dc.date.issued2014-06-
dc.identifierFernández Lahore, Marcelo; Grasselli, Mariano; Dsouza, Roy N.; Singh, Naveen Kumar; High capacity cryogel-type adsorbents for protein purification; Elsevier Science; Journal of Chromatography - A; 1355; 6-2014; 143-148-
dc.identifier0021-9673-
dc.identifierhttp://hdl.handle.net/11336/31452-
dc.identifierCONICET Digital-
dc.identifierCONICET-
dc.identifier.urihttp://rodna.bn.gov.ar:8080/jspui/handle/bnmm/297623-
dc.descriptionCryogel bodies were modified to obtain epoxy groups by graft-copolymerization using both chemical and gamma irradiation initiation techniques. The free epoxy adsorbents were reacted further to introduce diethylaminoethanol (DEAE) functionalities. The resulting weak anion-exchange cryogel adsorbents showed dynamic binding capacities of ca. 27 ± 3 mg/mL, which was significantly higher than previously reported for this type of adsorbent material. Gamma irradiated grafting initiation showed a 4-fold higher capacity for proteins than chemical grafting initiation procedures. The phosphate capacity for these DEAE cryogels was 119 mmol/L and also showed similar column efficiency as compared to commercial adsorbents. The large pores in the cryogel structure ensure convective transport of the molecules to active binding sites located on the polymer-grafted surface of cryogels. However, as cryogels have relatively large pores (10–100 μm), the BET area available for surface activation is low, and consequently, the capacity of the cryogels is relatively low for biomolecules, especially when compared to commercial beaded adsorbents. Nevertheless, we have shown that gamma ray mediated surface grafting of cryogel matrices greatly enhance their functional and adsorptive properties.-
dc.descriptionFil: Singh, Naveen Kumar. Universitat Bremen. School of Enigineerring and Science Jacobs; Alemania-
dc.descriptionFil: Dsouza, Roy N.. Universitat Bremen. School of Enigineerring and Science Jacobs; Alemania-
dc.descriptionFil: Grasselli, Mariano. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina-
dc.descriptionFil: Fernández Lahore, Marcelo. Universitat Bremen. School of Enigineerring and Science Jacobs; Alemania-
dc.formatapplication/pdf-
dc.formatapplication/pdf-
dc.languageeng-
dc.publisherElsevier Science-
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.chroma.2014.06.008-
dc.relationinfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0021967314008899-
dc.rightsinfo:eu-repo/semantics/openAccess-
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/-
dc.sourcereponame:CONICET Digital (CONICET)-
dc.sourceinstname:Consejo Nacional de Investigaciones Científicas y Técnicas-
dc.sourceinstacron:CONICET-
dc.source.urihttp://hdl.handle.net/11336/31452-
dc.subjectPROTEIN CHROMATOGRAPHY-
dc.subjectMONOLITHS-
dc.subjectMEGAPOROUS CRYOGELS-
dc.subjectWEAK ANION-EXCHANGE-
dc.subjectBiotecnología Industrial-
dc.subjectBiotecnología Industrial-
dc.subjectINGENIERÍAS Y TECNOLOGÍAS-
dc.titleHigh capacity cryogel-type adsorbents for protein purification-
dc.typeinfo:eu-repo/semantics/article-
dc.typeinfo:eu-repo/semantics/publishedVersion-
dc.typeinfo:ar-repo/semantics/articulo-
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