Registro completo de metadatos
| Campo DC | Valor | Lengua/Idioma |
|---|---|---|
| dc.provenance | CONICET | - |
| dc.creator | Aquino Esperanza, Jose A. | - |
| dc.creator | Aguirre, María Victoria | - |
| dc.creator | Aispuru, Gualberto R. | - |
| dc.creator | Lettieri, Carolina N. | - |
| dc.creator | Juaristi, Julian Antonio | - |
| dc.creator | Alvarez, Mirta Alba | - |
| dc.creator | Brandan, Nora Cristina | - |
| dc.date | 2018-09-18T22:44:52Z | - |
| dc.date | 2018-09-18T22:44:52Z | - |
| dc.date | 2008-10 | - |
| dc.date | 2018-09-18T14:05:15Z | - |
| dc.date.accessioned | 2019-04-29T15:37:02Z | - |
| dc.date.available | 2019-04-29T15:37:02Z | - |
| dc.date.issued | 2008-10 | - |
| dc.identifier | Aquino Esperanza, Jose A.; Aguirre, María Victoria; Aispuru, Gualberto R.; Lettieri, Carolina N.; Juaristi, Julian Antonio; et al.; In vivo 5-flourouracil-induced apoptosis on murine thymocytes: Involvement of FAS, Bax and Caspase3; Springer; Cell Biology And Toxicology; 24; 5; 10-2008; 411-422 | - |
| dc.identifier | 0742-2091 | - |
| dc.identifier | http://hdl.handle.net/11336/60203 | - |
| dc.identifier | CONICET Digital | - |
| dc.identifier | CONICET | - |
| dc.identifier.uri | http://rodna.bn.gov.ar:8080/jspui/handle/bnmm/297811 | - |
| dc.description | Apoptosis is a highly regulated and programmed cell breakdown process characterized by numerous changes. It was reported as the major mechanism of anticancer drug-induced cells death. Unfortunately, many of these drugs are non-specific and cause severe side effects. The effects of 5-flourouracil (5-FU) on the apoptotic events in normal murine thymus were evaluated using an in vivo model. A single dose of 5-FU (150 mg/kg ip) was injected to CF-1 mice. A multiparametric analysis of thymic weight, cellularity, viability, architectural organization, apoptosis, DNA fragmentation, and the expression of several apoptotic proteins was evaluated in 10 days time-course study post-5-FU dosing. Total organ weights, thymocyte counts, and cell viabilities diminished drastically from the second day. The thymus architecture assessed through electron scanning microscopy revealed deep alterations and the lost of cell-cell contact between the first and the third days. DNA fragmentation and apoptotic indexes (May Grünwald Giemsa staining, double flourescent dyes, and TdT-mediated dUTP nick-end labeling assay) revealed that cell death was maximal on the second day (three times over control). Furthermore, the pro-apoptotic proteins FAS and Bax were strongly up-regulated during the first 2 days. The aforementioned morphological and biochemical changes were also accompanied within the same period by caspase 3 activation. This study revealed that in vivo apoptosis in normal thymus after 5-FU administration is related to FAS, Bax, and Caspase 3 co-expressions under the current experimental conditions, these findings, therefore, contribute to a new insight into the molecular mechanisms involved during 5-FU administration upon the thymus and the possible events committed in the lymphophenia associated with chemotherapy. © 2008 Springer Science+Business Media B.V. | - |
| dc.description | Fil: Aquino Esperanza, Jose A.. Universidad Nacional del Nordeste. Facultad de Medicina; Argentina | - |
| dc.description | Fil: Aguirre, María Victoria. Universidad Nacional del Nordeste. Facultad de Medicina; Argentina | - |
| dc.description | Fil: Aispuru, Gualberto R.. Universidad Nacional del Nordeste. Facultad de Medicina; Argentina | - |
| dc.description | Fil: Lettieri, Carolina N.. Universidad Nacional del Nordeste. Facultad de Medicina; Argentina | - |
| dc.description | Fil: Juaristi, Julian Antonio. Universidad Nacional del Nordeste. Facultad de Medicina; Argentina | - |
| dc.description | Fil: Alvarez, Mirta Alba. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Nordeste. Facultad de Medicina; Argentina | - |
| dc.description | Fil: Brandan, Nora Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Nordeste. Facultad de Medicina; Argentina | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.language | eng | - |
| dc.publisher | Springer | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1007/s10565-008-9056-z | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs10565-008-9056-z | - |
| dc.rights | info:eu-repo/semantics/restrictedAccess | - |
| dc.rights | https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ | - |
| dc.source | reponame:CONICET Digital (CONICET) | - |
| dc.source | instname:Consejo Nacional de Investigaciones Científicas y Técnicas | - |
| dc.source | instacron:CONICET | - |
| dc.source.uri | http://hdl.handle.net/11336/60203 | - |
| dc.subject | 5-FU | - |
| dc.subject | APOPTOSIS | - |
| dc.subject | BAX | - |
| dc.subject | CASPASE 3 | - |
| dc.subject | FAS | - |
| dc.subject | MURINE THYMUS | - |
| dc.subject | Inmunología | - |
| dc.subject | Medicina Básica | - |
| dc.subject | CIENCIAS MÉDICAS Y DE LA SALUD | - |
| dc.title | In vivo 5-flourouracil-induced apoptosis on murine thymocytes: Involvement of FAS, Bax and Caspase3 | - |
| dc.type | info:eu-repo/semantics/article | - |
| dc.type | info:eu-repo/semantics/publishedVersion | - |
| dc.type | info:ar-repo/semantics/articulo | - |
| Aparece en las colecciones: | CONICET | |
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