Registro completo de metadatos
| Campo DC | Valor | Lengua/Idioma |
|---|---|---|
| dc.provenance | CONICET | - |
| dc.creator | Mautner, Martín E. | - |
| dc.creator | Perez Santangelo, Agustin | - |
| dc.creator | Corti Bielsa, Rodrigo M. | - |
| dc.creator | Sala, Andrea | - |
| dc.creator | Ginart, Santiago | - |
| dc.creator | Corach, Daniel | - |
| dc.date | 2018-06-15T17:30:19Z | - |
| dc.date | 2018-06-15T17:30:19Z | - |
| dc.date | 2017-11 | - |
| dc.date | 2018-06-13T15:09:14Z | - |
| dc.date.accessioned | 2019-04-29T15:38:05Z | - |
| dc.date.available | 2019-04-29T15:38:05Z | - |
| dc.date.issued | 2017-11 | - |
| dc.identifier | Mautner, Martín E.; Perez Santangelo, Agustin; Corti Bielsa, Rodrigo M.; Sala, Andrea; Ginart, Santiago; et al.; Using long ssDNA polynucleotides to amplify STRs loci in degraded DNA samples; Public Library of Science; Plos One; 12; 11; 11-2017; 1-20 | - |
| dc.identifier | 1932-6203 | - |
| dc.identifier | http://hdl.handle.net/11336/48810 | - |
| dc.identifier | CONICET Digital | - |
| dc.identifier | CONICET | - |
| dc.identifier.uri | http://rodna.bn.gov.ar:8080/jspui/handle/bnmm/298172 | - |
| dc.description | Obtaining informative short tandem repeat (STR) profiles from degraded DNA samples is a challenging task usually undermined by locus or allele dropouts and peak-high imbalances observed in capillary electrophoresis (CE) electropherograms, especially for those markers with large amplicon sizes. We hereby show that the current STR assays may be greatly improved for the detection of genetic markers in degraded DNA samples by using long single stranded DNA polynucleotides (ssDNA polynucleotides) as surrogates for PCR primers. These long primers allow a closer annealing to the repeat sequences, thereby reducing the length of the template required for the amplification in fragmented DNA samples, while at the same time rendering amplicons of larger sizes suitable for multiplex assays. We also demonstrate that the annealing of long ssDNA polynucleotides does not need to be fully complementary in the 5’ region of the primers, thus allowing for the design of practically any long primer sequence for developing new multiplex assays. Furthermore, genotyping of intact DNA samples could also benefit from utilizing long primers since their close annealing to the target STR sequences may overcome wrong profiling generated by insertions/deletions present between the STR region and the annealing site of the primers. Additionally, long ssDNA polynucleotides might be utilized in multiplex PCR assays for other types of degraded or fragmented DNA, e.g. circulating, cell-free DNA (ccfDNA). | - |
| dc.description | Fil: Mautner, Martín E.. Biodynamics SRL; Argentina | - |
| dc.description | Fil: Perez Santangelo, Agustin. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Torcuato Di Tella; Argentina | - |
| dc.description | Fil: Corti Bielsa, Rodrigo M.. Biodynamics SRL; Argentina | - |
| dc.description | Fil: Sala, Andrea. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina | - |
| dc.description | Fil: Ginart, Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina | - |
| dc.description | Fil: Corach, Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.language | eng | - |
| dc.publisher | Public Library of Science | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1371/journal.pone.0187190 | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0187190 | - |
| dc.rights | info:eu-repo/semantics/openAccess | - |
| dc.rights | https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ | - |
| dc.source | reponame:CONICET Digital (CONICET) | - |
| dc.source | instname:Consejo Nacional de Investigaciones Científicas y Técnicas | - |
| dc.source | instacron:CONICET | - |
| dc.source.uri | http://hdl.handle.net/11336/48810 | - |
| dc.subject | long ssDNA polynucleotides | - |
| dc.subject | degraded DNA | - |
| dc.subject | forensic | - |
| dc.subject | STR | - |
| dc.subject | Otras Ciencias Biológicas | - |
| dc.subject | Ciencias Biológicas | - |
| dc.subject | CIENCIAS NATURALES Y EXACTAS | - |
| dc.title | Using long ssDNA polynucleotides to amplify STRs loci in degraded DNA samples | - |
| dc.type | info:eu-repo/semantics/article | - |
| dc.type | info:eu-repo/semantics/publishedVersion | - |
| dc.type | info:ar-repo/semantics/articulo | - |
| Aparece en las colecciones: | CONICET | |
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