Registro completo de metadatos
| Campo DC | Valor | Lengua/Idioma |
|---|---|---|
| dc.provenance | CONICET | - |
| dc.creator | Barrera, Antonio Daniel | - |
| dc.creator | Garcia, Elina Vanesa | - |
| dc.creator | Hamdi, Meriem | - |
| dc.creator | Sánchez Calabuig, María Jesús | - |
| dc.creator | López Cardona, Ángela Patricia | - |
| dc.creator | Fonseca Balvís, Noelia | - |
| dc.creator | Rizos, Dimitrios | - |
| dc.creator | Gutiérrez Adan, Alfonso | - |
| dc.date | 2018-08-01T22:02:59Z | - |
| dc.date | 2018-08-01T22:02:59Z | - |
| dc.date | 2017-04 | - |
| dc.date | 2018-07-30T13:48:38Z | - |
| dc.date.accessioned | 2019-04-29T15:38:14Z | - |
| dc.date.available | 2019-04-29T15:38:14Z | - |
| dc.date.issued | 2017-04 | - |
| dc.identifier | Barrera, Antonio Daniel; Garcia, Elina Vanesa; Hamdi, Meriem; Sánchez Calabuig, María Jesús; López Cardona, Ángela Patricia; et al.; Embryo culture in presence of oviductal fluid induces DNA methylation changes in bovine blastocysts; BioScientifica; Reproduction; 154; 1; 4-2017; 1-12 | - |
| dc.identifier | 1470-1626 | - |
| dc.identifier | http://hdl.handle.net/11336/53858 | - |
| dc.identifier | CONICET Digital | - |
| dc.identifier | CONICET | - |
| dc.identifier.uri | http://rodna.bn.gov.ar:8080/jspui/handle/bnmm/298211 | - |
| dc.description | During the transit through the oviduct, the early embryo initiates an extensive DNA methylation reprogramming of its genome. Given that these epigenetic modifications are susceptible to environmental factors, components present in the oviductal milieu could affect the DNA methylation marks of the developing embryo. The aim of this study was to examine if culture of bovine embryos with oviductal fluid (OF) can induce DNA methylation changes at specific genomic regions in the resulting blastocysts. In vitro produced zygotes were cultured in medium with 3 mg/mL bovine serum albumin (BSA) or 1.25% OF added at the one- to 16-cell stage (OF1-16), one- to 8-cell stage (OF1-8) or 8- to 16-cell stage (OF8-16), and then were cultured until Day 8 in medium with 3 mg/mL BSA. Genomic regions in four developmentally important genes (MTERF2, ABCA7, OLFM1, GMDS) and within LINE-1 retrotransposons were selected for methylation analysis by bisulfite sequencing on Day 7-8 blastocysts. Blastocysts derived from OF1-16 group showed lower CpG methylation levels in MTERF2 and ABCA7 compared with the BSA group. However, CpG sites within MTERF2, ABCA7 and OLFM1 showed higher methylation levels in groups OF1-8 and OF8-16 than in OF1-16. For LINE-1 elements, higher CpG methylation levels were observed in blastocysts from the OF1-16 group than in the other experimental groups. In correlation with the methylation changes observed, mRNA expression level of MTERF2 was increased, while LINE-1 showed a decreased expression in blastocysts from OF1-16 group. Our results suggest that embryos show transient sensitivity to OF at early stages, which is reflected by specific methylation changes at the blastocyst stage. | - |
| dc.description | Fil: Barrera, Antonio Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina | - |
| dc.description | Fil: Garcia, Elina Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina | - |
| dc.description | Fil: Hamdi, Meriem. Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria. Departamento de Reproducción Animal; España | - |
| dc.description | Fil: Sánchez Calabuig, María Jesús. Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria. Departamento de Reproducción Animal; España | - |
| dc.description | Fil: López Cardona, Ángela Patricia. Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria. Departamento de Reproducción Animal; España | - |
| dc.description | Fil: Fonseca Balvís, Noelia. Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria. Departamento de Reproducción Animal; España | - |
| dc.description | Fil: Rizos, Dimitrios. Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria. Departamento de Reproducción Animal; España | - |
| dc.description | Fil: Gutiérrez Adan, Alfonso. Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria. Departamento de Reproducción Animal; España | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.language | eng | - |
| dc.publisher | BioScientifica | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/url/http://www.reproduction-online.org/content/154/1/1 | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1530/REP-16-0651 | - |
| dc.rights | info:eu-repo/semantics/openAccess | - |
| dc.rights | https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ | - |
| dc.source | reponame:CONICET Digital (CONICET) | - |
| dc.source | instname:Consejo Nacional de Investigaciones Científicas y Técnicas | - |
| dc.source | instacron:CONICET | - |
| dc.source.uri | http://hdl.handle.net/11336/53858 | - |
| dc.subject | BOVINE | - |
| dc.subject | BLASTOCYST | - |
| dc.subject | DNA METHYLATION | - |
| dc.subject | OVIDUCT | - |
| dc.subject | OVIDUCTAL FLUID | - |
| dc.subject | Otras Ciencias Biológicas | - |
| dc.subject | Ciencias Biológicas | - |
| dc.subject | CIENCIAS NATURALES Y EXACTAS | - |
| dc.title | Embryo culture in presence of oviductal fluid induces DNA methylation changes in bovine blastocysts | - |
| dc.type | info:eu-repo/semantics/article | - |
| dc.type | info:eu-repo/semantics/publishedVersion | - |
| dc.type | info:ar-repo/semantics/articulo | - |
| Aparece en las colecciones: | CONICET | |
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