Registro completo de metadatos
| Campo DC | Valor | Lengua/Idioma |
|---|---|---|
| dc.provenance | CONICET | - |
| dc.creator | Carou, María Clara | - |
| dc.creator | Cruzans, Paula Romina | - |
| dc.creator | Maruri, Alejandro | - |
| dc.creator | Farina, Mariana | - |
| dc.creator | Fiorito, Carla Daniela | - |
| dc.creator | Olea, Gabriela Beatriz | - |
| dc.creator | Lombardo, Daniel Marcelo | - |
| dc.date | 2018-04-25T13:46:19Z | - |
| dc.date | 2018-04-25T13:46:19Z | - |
| dc.date | 2017-06 | - |
| dc.date | 2018-04-05T18:19:20Z | - |
| dc.date.accessioned | 2019-04-29T15:38:18Z | - |
| dc.date.available | 2019-04-29T15:38:18Z | - |
| dc.date.issued | 2017-06 | - |
| dc.identifier | Carou, María Clara; Cruzans, Paula Romina; Maruri, Alejandro; Farina, Mariana; Fiorito, Carla Daniela; et al.; Apoptosis of bovine granulosa cells: Intracellular pathways and differentiation.; Elsevier Gmbh, Urban & Fischer Verlag; Acta Histochemica; 119; 1; 6-2017; 462-470 | - |
| dc.identifier | 0065-1281 | - |
| dc.identifier | http://hdl.handle.net/11336/43415 | - |
| dc.identifier | CONICET Digital | - |
| dc.identifier | CONICET | - |
| dc.identifier.uri | http://rodna.bn.gov.ar:8080/jspui/handle/bnmm/298231 | - |
| dc.description | Follicular atresia in granulosa and theca cells occurs by apoptosis through weak hormonal stimulation. We have previously proposed anin vitro model to study this process by inducing apoptosis in BGC-1, a bovine granulosa cell line, and in primary cultures from ovaries with or without corpus luteum (CPGB+ and CPGB−,respectively), with different doses of gonadotropin releasing hormone (GnRH) analogs (leuprolide acetate (LA) as agonist and antide as antagonist). BGC-1 represent immature granulosa cells, whereas CPGB represent different degrees of luteinization. Our aim was to evaluate the intracellular pathways involved in the GnRH regulation of apoptosis in BGC-1. Treatment with LA 100 nM but not with antide led to an increase in BAX over BCL-2 expression, showing antagonism of antide. All treatments inhibited phospholipase-D (PLD) activity compared to control, implying agonist behavior of antide. Progesteronein vitro production and 3β-hydroxysteroid dehydrogenase (3β-HSD) expression revealed different degrees of luteinization: BGC-1 were immature, whereas CPGB+ were lessdifferentiated than CPGB−. We concluded that LA-induced apoptosis in BGC-1 occurs by activation of the mitochondrial pathway and by inhibition of PLD activity and that antide might work both as an antagonist of the intrinsic pathway and as an agonist of the extrinsic protection pathway by inhibiting PLD activity. | - |
| dc.description | Fil: Carou, María Clara. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina | - |
| dc.description | Fil: Cruzans, Paula Romina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina | - |
| dc.description | Fil: Maruri, Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina | - |
| dc.description | Fil: Farina, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina | - |
| dc.description | Fil: Fiorito, Carla Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico; Argentina | - |
| dc.description | Fil: Olea, Gabriela Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Nordeste; Argentina | - |
| dc.description | Fil: Lombardo, Daniel Marcelo. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.language | eng | - |
| dc.publisher | Elsevier Gmbh, Urban & Fischer Verlag | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.acthis.2017.04.010 | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0065128117301356 | - |
| dc.rights | info:eu-repo/semantics/restrictedAccess | - |
| dc.rights | https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ | - |
| dc.source | reponame:CONICET Digital (CONICET) | - |
| dc.source | instname:Consejo Nacional de Investigaciones Científicas y Técnicas | - |
| dc.source | instacron:CONICET | - |
| dc.source.uri | http://hdl.handle.net/11336/43415 | - |
| dc.subject | BGC-1 | - |
| dc.subject | GnRH analogs | - |
| dc.subject | Apoptosis | - |
| dc.subject | Bovine granulosa cells | - |
| dc.subject | Differentiation | - |
| dc.subject | 3β-HSD | - |
| dc.subject | Otras Ciencias Veterinarias | - |
| dc.subject | Ciencias Veterinarias | - |
| dc.subject | CIENCIAS AGRÍCOLAS | - |
| dc.title | Apoptosis of bovine granulosa cells: Intracellular pathways and differentiation. | - |
| dc.type | info:eu-repo/semantics/article | - |
| dc.type | info:eu-repo/semantics/publishedVersion | - |
| dc.type | info:ar-repo/semantics/articulo | - |
| Aparece en las colecciones: | CONICET | |
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