Registro completo de metadatos
| Campo DC | Valor | Lengua/Idioma |
|---|---|---|
| dc.provenance | CONICET | - |
| dc.creator | Rinaldi, Jimena Julieta | - |
| dc.creator | Ocampo, Josefina | - |
| dc.creator | Rossi, Silvia Graciela | - |
| dc.creator | Moreno, Silvia Margarita | - |
| dc.date | 2018-05-09T15:38:34Z | - |
| dc.date | 2018-05-09T15:38:34Z | - |
| dc.date | 2008-12 | - |
| dc.date | 2018-04-05T19:23:57Z | - |
| dc.date.accessioned | 2019-04-29T15:38:30Z | - |
| dc.date.available | 2019-04-29T15:38:30Z | - |
| dc.date.issued | 2008-12 | - |
| dc.identifier | Rinaldi, Jimena Julieta; Ocampo, Josefina; Rossi, Silvia Graciela; Moreno, Silvia Margarita; A novel activating effect of the regulatory subunit of protein kinase A on catalytic subunit activity; Elsevier Science Inc; Archives of Biochemistry and Biophysics; 480; 2; 12-2008; 95-103 | - |
| dc.identifier | 0003-9861 | - |
| dc.identifier | http://hdl.handle.net/11336/44587 | - |
| dc.identifier | 1096-0384 | - |
| dc.identifier | CONICET Digital | - |
| dc.identifier | CONICET | - |
| dc.identifier.uri | http://rodna.bn.gov.ar:8080/jspui/handle/bnmm/298315 | - |
| dc.description | The strength of the interaction between the catalytic and regulatory subunits in protein kinase A differs among species. The linker region from regulatory subunits is non-conserved. To evaluate the participation of this region in the interaction with the catalytic subunit, we have assayed its effect on the enzymatic properties of the catalytic subunit. Protein kinase A from three fungi, Mucor rouxii, Mucor circinelloides and Saccharomyces cerevisiae have been chosen as models. The R-C interaction is explored by using synthetic peptides of 8, 18 and 47 amino acids, corresponding to the R subunit autophosphorylation site plus a variable region toward the N terminus (0, 10, or 39 residues). The K(m) of the catalytic subunits decreased with the length of the peptide, while the V(max) increased. Viscosity studies identified product release as the rate limiting step for phosphorylation of the longer peptides. Pseudosubstrate derivatives of the 18 residue peptides did not display a competitive inhibition behavior toward either kemptide or a bona fide protein substrate since, at low relative pseudosubstrate/substrate concentration, stimulation of kemptide or protein substrate phosphorylation was observed. The behavior was mimicked by intact R. We conclude that in addition to its negative regulatory role, the R subunit stimulates C activity via distal interactions. | - |
| dc.description | Fil: Rinaldi, Jimena Julieta. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina | - |
| dc.description | Fil: Ocampo, Josefina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina | - |
| dc.description | Fil: Rossi, Silvia Graciela. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina | - |
| dc.description | Fil: Moreno, Silvia Margarita. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.language | eng | - |
| dc.publisher | Elsevier Science Inc | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0003986108004517 | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1016/j.abb.2008.09.014 | - |
| dc.rights | info:eu-repo/semantics/restrictedAccess | - |
| dc.rights | https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ | - |
| dc.source | reponame:CONICET Digital (CONICET) | - |
| dc.source | instname:Consejo Nacional de Investigaciones Científicas y Técnicas | - |
| dc.source | instacron:CONICET | - |
| dc.source.uri | http://hdl.handle.net/11336/44587 | - |
| dc.subject | Protein kinase A | - |
| dc.subject | Peptides | - |
| dc.subject | linker domain I | - |
| dc.subject | Substrates | - |
| dc.subject | Regulator subunit | - |
| dc.subject | Inhibition | - |
| dc.subject | Actvation | - |
| dc.subject | Otras Ciencias Químicas | - |
| dc.subject | Ciencias Químicas | - |
| dc.subject | CIENCIAS NATURALES Y EXACTAS | - |
| dc.title | A novel activating effect of the regulatory subunit of protein kinase A on catalytic subunit activity | - |
| dc.type | info:eu-repo/semantics/article | - |
| dc.type | info:eu-repo/semantics/publishedVersion | - |
| dc.type | info:ar-repo/semantics/articulo | - |
| Aparece en las colecciones: | CONICET | |
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