Registro completo de metadatos
| Campo DC | Valor | Lengua/Idioma |
|---|---|---|
| dc.provenance | CONICET | - |
| dc.creator | Corradi, Jeremias | - |
| dc.creator | Andersen, Natalia Denise | - |
| dc.creator | Bouzat, Cecilia Beatriz | - |
| dc.date | 2016-07-12T19:32:49Z | - |
| dc.date | 2016-07-12T19:32:49Z | - |
| dc.date | 2011-01 | - |
| dc.date | 2016-07-05T18:47:19Z | - |
| dc.date.accessioned | 2019-04-29T15:38:31Z | - |
| dc.date.available | 2019-04-29T15:38:31Z | - |
| dc.date.issued | 2011-01 | - |
| dc.identifier | Corradi, Jeremias; Andersen, Natalia Denise; Bouzat, Cecilia Beatriz; A Novel Mechanism of Modulation of 5-HT3A Receptors by Hydrocortisone; Cell Press; Biophysical Journal; 100; 1; 1-2011; 42-51 | - |
| dc.identifier | 0006-3495 | - |
| dc.identifier | http://hdl.handle.net/11336/6459 | - |
| dc.identifier.uri | http://rodna.bn.gov.ar:8080/jspui/handle/bnmm/298323 | - |
| dc.description | Modulation of Cys-loop receptors by steroids is of physiological and therapeutical relevance. Nonetheless, its molecular mechanism has not been elucidated for serotonin (5-HT) type 3 receptors. We deciphered the mechanism of action of hydrocortisone (HC) at 5-HT type 3A receptors. Single-channel currents from the high-conductance form (∼4.7 pA, −70 mV) appear as a series of long opening events forming bursts, which group into long clusters. Although they are very infrequent, subconductance events (∼2.4 pA) are detected within clusters. HC produces a significant concentration-dependent reduction in open and burst durations, demonstrating open-channel block. In addition, it increases the appearance of subconductance levels in a concentration- and slightly voltage-dependent manner. The amplitude of the subconductance level does not change with HC concentration and its open duration is briefer than that of full amplitude events, indicating lower open-channel stability. Dual effects are distinguished from macroscopic responses: HC reduces amplitude by acting from either open or closed states, and it increases decay rates from the open state. Thus, HC acts as a negative modulator of 5-HT type 3A receptors by different mechanisms: It acts as an open-channel blocker and it favors opening to a preexisting subconductance level. The latter constitutes a novel, to our knowledge, mechanism of channel modulation, which might be applicable to other steroids and channels. | - |
| dc.description | Fil: Corradi, Jeremias. Consejo Nacional de Investigaciones Cientificas y Técnicas. Centro Científico Tecnológico Bahia Blanca. Instituto de Investigaciones Bioquímicas Bahia Blanca (i); Argentina. Universidad Nacional del Sur; Argentina | - |
| dc.description | Fil: Andersen, Natalia Denise. Consejo Nacional de Investigaciones Cientificas y Técnicas. Centro Científico Tecnológico Bahia Blanca. Instituto de Investigaciones Bioquímicas Bahia Blanca (i); Argentina. Universidad Nacional del Sur; Argentina | - |
| dc.description | Fil: Bouzat, Cecilia Beatriz. Consejo Nacional de Investigaciones Cientificas y Técnicas. Centro Científico Tecnológico Bahia Blanca. Instituto de Investigaciones Bioquímicas Bahia Blanca (i); Argentina. Universidad Nacional del Sur; Argentina | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.language | eng | - |
| dc.publisher | Cell Press | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bpj.2010.10.046 | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0006349510013640 | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.bpj.2010.10.046 | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/url/http://www.cell.com/biophysj/abstract/S0006-3495(10)01364-0 | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/url/http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3010835/ | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/pmid/PMC3010835 | - |
| dc.rights | info:eu-repo/semantics/restrictedAccess | - |
| dc.rights | https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ | - |
| dc.source | reponame:CONICET Digital (CONICET) | - |
| dc.source | instname:Consejo Nacional de Investigaciones Científicas y Técnicas | - |
| dc.source | instacron:CONICET | - |
| dc.source.uri | http://hdl.handle.net/11336/6459 | - |
| dc.subject | 5-HT3 RECEPTOR | - |
| dc.subject | STEROIDS | - |
| dc.subject | LIGAND-GATED ION CHANNELS | - |
| dc.subject | PATCH-CLAMP | - |
| dc.subject | Biofísica | - |
| dc.subject | Ciencias Biológicas | - |
| dc.subject | CIENCIAS NATURALES Y EXACTAS | - |
| dc.title | A Novel Mechanism of Modulation of 5-HT3A Receptors by Hydrocortisone | - |
| dc.type | info:eu-repo/semantics/article | - |
| dc.type | info:eu-repo/semantics/publishedVersion | - |
| dc.type | info:ar-repo/semantics/articulo | - |
| Aparece en las colecciones: | CONICET | |
Ficheros en este ítem:
No hay ficheros asociados a este ítem.