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dc.provenanceCONICET-
dc.creatorGiambelluca, Miriam Soledad-
dc.creatorGende, Oscar Alfredo-
dc.date2018-09-26T15:29:39Z-
dc.date2018-09-26T15:29:39Z-
dc.date2011-
dc.date2018-09-10T16:43:12Z-
dc.date.accessioned2019-04-29T15:38:34Z-
dc.date.available2019-04-29T15:38:34Z-
dc.date.issued2011-
dc.identifierGiambelluca, Miriam Soledad; Gende, Oscar Alfredo; Cl-/HCO3- exchange activity in fMLP-stimulated human neutrophils; Academic Press Inc Elsevier Science; Biochemical and Biophysical Research Communications; 409; 3; 2011; 567-571-
dc.identifier0006-291X-
dc.identifierhttp://hdl.handle.net/11336/60871-
dc.identifierCONICET Digital-
dc.identifierCONICET-
dc.identifier.urihttp://rodna.bn.gov.ar:8080/jspui/handle/bnmm/298342-
dc.descriptionIt is well known that chemotactic agents active Na+/H+ exchanger, increasing intracellular pH of neutrophils, but their effect on bicarbonate transporters have not been established yet. To study the effect of fMLP on the activity of Cl/HCO3 exchange, the rate of pH recovery after acute Cl readmission in cell subjected to an alkaline load by CO2 washout in a Cl-free medium was measured. The activity of the exchanger was reduced to 72% of control when cells were pre-incubated for 5 min with 0.1 lM fMLP and reached 48% of control in steady state after acute exposure. After extracellular bicarbonate or TMA addition the rate recovery of intracellular pH was reduce at 72% and at 84%, respectively. The inhibitory effect on the intracellular pH recovery was not affected by blockers of Na+/H+ xchange. We conclude from these studies that an increase of pHi produced for this chemotactic agent is facilitated by the simultaneous activation of Na+/H+ exchange and inhibition of Cl/HCO 3 exchange in neutrophils.-
dc.descriptionFil: Giambelluca, Miriam Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones Cardiovasculares ; Argentina-
dc.descriptionFil: Gende, Oscar Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones Cardiovasculares ; Argentina-
dc.formatapplication/pdf-
dc.formatapplication/pdf-
dc.languageeng-
dc.publisherAcademic Press Inc Elsevier Science-
dc.relationinfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0006291X11008060-
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.bbrc.2011.05.046-
dc.rightsinfo:eu-repo/semantics/restrictedAccess-
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/-
dc.sourcereponame:CONICET Digital (CONICET)-
dc.sourceinstname:Consejo Nacional de Investigaciones Científicas y Técnicas-
dc.sourceinstacron:CONICET-
dc.source.urihttp://hdl.handle.net/11336/60871-
dc.subjectCHLORIDE-BICARBONATE EXCHANGER-
dc.subjectNEUTROPHILS-
dc.subjectINTRACELULAR PH-
dc.subjectCHLORIDE-
dc.subjectBioquímica y Biología Molecular-
dc.subjectMedicina Básica-
dc.subjectCIENCIAS MÉDICAS Y DE LA SALUD-
dc.titleCl-/HCO3- exchange activity in fMLP-stimulated human neutrophils-
dc.typeinfo:eu-repo/semantics/article-
dc.typeinfo:eu-repo/semantics/publishedVersion-
dc.typeinfo:ar-repo/semantics/articulo-
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