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dc.provenanceCONICET-
dc.creatorAcardi, Soraya Alejandra-
dc.creatorRago, María Virginia-
dc.creatorLiotta, Domingo Javier-
dc.creatorFernandez Duque, Eduardo-
dc.creatorSalomon, Oscar Daniel-
dc.date2016-09-08T14:52:44Z-
dc.date2016-09-08T14:52:44Z-
dc.date2013-03-
dc.date2016-09-02T18:06:28Z-
dc.date.accessioned2019-04-29T15:38:39Z-
dc.date.available2019-04-29T15:38:39Z-
dc.date.issued2013-03-
dc.identifierAcardi, Soraya Alejandra; Rago, María Virginia; Liotta, Domingo Javier; Fernandez Duque, Eduardo; Salomon, Oscar Daniel; Leishmania (Viannia) DNA detection by PCR-RFLP and sequencing in free-ranging owl monkeys (Aotus azarai azarai) from Formosa, Argentina; Elsevier Science; Veterinary Parasitology; 193; 1-3; 3-2013; 256-259-
dc.identifier0304-4017-
dc.identifierhttp://hdl.handle.net/11336/7554-
dc.identifier.urihttp://rodna.bn.gov.ar:8080/jspui/handle/bnmm/298380-
dc.descriptionAmerican Cutaneous Leishmaniasis (ACL) is caused by protozoan parasites of the Leishmania genus, and transmitted by females of the Phlebotominae family. The role of wild and domestic hosts in the cycle of Leishmania is still unknown. ACL is endemic in the province of Formosa where Nyssomyia neivai was the most abundant species in several captures and 31 cumulative ACL human cases were reported between 2005 and 2011 in the province. The present report describes the detection, by PCR-RFLP and confirmed by sequencing, of subgenus Leishmania (Viannia) DNA in four free-ranging owl monkeys (Aotus azarai azarai) from Formosa Province. The sequence amplified was the mini-exon gene present in tandem repeats in all species of the Leishmania genus from buffy coat samples. The absence of inhibitors in the samples was checked by a β-globin protocol originally designed to amplify the human β-globin gene. However, other free-ranging primates were found with natural infections of L. (V) braziliensis complex and Leishmania (Viannia) subgenus by parasitological means in America. To the best of our knowledge, there are no published reports on detection of subgenus Leishmania (Viannia) DNA by PCR-RFLP in argentinean free-ranging primates. Additional eco-epidemiological and parasitological studies are necessary to confirm owl monkeys, or any other natural infected mammal species detected by PCR, as a reservoir, incidental host or to propose it as an animal model for research on this topic.-
dc.descriptionFil: Acardi, Soraya Alejandra. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Laboratorio de Biología Molecular Aplicada; Argentina. Ministerio de Salud. Instituto Nacional de Medicina Tropical; Argentina-
dc.descriptionFil: Rago, María Virginia. Wildlife Conservation Society; Estados Unidos-
dc.descriptionFil: Liotta, Domingo Javier. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Laboratorio de Biología Molecular Aplicada; Argentina. Ministerio de Salud. Instituto Nacional de Medicina Tropical; Argentina-
dc.descriptionFil: Fernandez Duque, Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Centro de Ecologia Aplicada del Litoral (i); Argentina. University of Pennsylvania; Estados Unidos-
dc.descriptionFil: Salomon, Oscar Daniel. Instituto Nacional de Medicina Tropical, Misiones; Argentina-
dc.formatapplication/pdf-
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dc.formatapplication/pdf-
dc.formatapplication/pdf-
dc.languageeng-
dc.publisherElsevier Science-
dc.relationinfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0304401712006607-
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.vetpar.2012.12.012-
dc.rightsinfo:eu-repo/semantics/restrictedAccess-
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/-
dc.sourcereponame:CONICET Digital (CONICET)-
dc.sourceinstname:Consejo Nacional de Investigaciones Científicas y Técnicas-
dc.sourceinstacron:CONICET-
dc.source.urihttp://hdl.handle.net/11336/7554-
dc.subjectLEISHMANIA-
dc.subjectDNA-
dc.subjectPRIMATES-
dc.subjectBiología Celular, Microbiología-
dc.subjectCiencias Biológicas-
dc.subjectCIENCIAS NATURALES Y EXACTAS-
dc.titleLeishmania (Viannia) DNA detection by PCR-RFLP and sequencing in free-ranging owl monkeys (Aotus azarai azarai) from Formosa, Argentina-
dc.typeinfo:eu-repo/semantics/article-
dc.typeinfo:eu-repo/semantics/publishedVersion-
dc.typeinfo:ar-repo/semantics/articulo-
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