Registro completo de metadatos
| Campo DC | Valor | Lengua/Idioma |
|---|---|---|
| dc.provenance | CONICET | - |
| dc.creator | Albarracín, Virginia Helena | - |
| dc.creator | Kraiselburd, Ivana | - |
| dc.creator | Bamann, Christian | - |
| dc.creator | Wood, Phillip G. | - |
| dc.creator | Bamberg, Ernst | - |
| dc.creator | Farias, Maria Eugenia | - |
| dc.creator | Gärtner, Wolfgang | - |
| dc.date | 2018-03-02T21:15:00Z | - |
| dc.date | 2018-03-02T21:15:00Z | - |
| dc.date | 2016-05 | - |
| dc.date | 2018-03-02T15:00:32Z | - |
| dc.date.accessioned | 2019-04-29T15:39:13Z | - |
| dc.date.available | 2019-04-29T15:39:13Z | - |
| dc.date.issued | 2016-05 | - |
| dc.identifier | Albarracín, Virginia Helena; Kraiselburd, Ivana; Bamann, Christian; Wood, Phillip G.; Bamberg, Ernst; et al.; Functional green-tuned proteorhodopsin from modern stromatolites; Public Library of Science; Plos One; 11; 5; 5-2016; 1-18; e0154962 | - |
| dc.identifier | 1932-6203 | - |
| dc.identifier | http://hdl.handle.net/11336/37774 | - |
| dc.identifier | CONICET Digital | - |
| dc.identifier | CONICET | - |
| dc.identifier.uri | http://rodna.bn.gov.ar:8080/jspui/handle/bnmm/298629 | - |
| dc.description | The sequenced genome of the poly-extremophile Exiguobacterium sp. S17, isolated from modern stromatolites at Laguna Socompa (3,570 m), a High-Altitude Andean Lake (HAAL) in Argentinean Puna revealed a putative proteorhodopsin-encoding gene. The HAAL area is exposed to the highest UV irradiation on Earth, making the microbial community living in the stromatolites test cases for survival strategies under extreme conditions. The heterologous expressed protein E17R from Exiguobacterium (248 amino acids, 85% sequence identity to its ortholog ESR from E. sibiricum) was assembled with retinal displaying an absorbance maximum at 524 nm, which makes it a member of the green-absorbing PR-subfamily. Titration down to low pH values (eventually causing partial protein denaturation) indicated a pK value between two and three. Global fitting of data from laser flash-induced absorption changes gave evidence for an early red-shifted intermediate (its formation being below the experimental resolution) that decayed (τ1 =3.5μs) into another red-shifted intermediate. This species decayed in a two-step process (τ2 =84μs, τ3 = 11 ms), to which the initial state of E17-PR was reformed with a kinetics of 2 ms. Proton transport capability of the HAAL protein was determined by BLM measurements. Additional blue light irradiation reduced the proton current, clearly identifying a blue light absorbing, M-like intermediate. The apparent absence of this intermediate is explained by closely matching formation and decay kinetics. | - |
| dc.description | Fil: Albarracín, Virginia Helena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Planta Piloto de Procesos Industriales Microbiologicos; Argentina | - |
| dc.description | Fil: Kraiselburd, Ivana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina | - |
| dc.description | Fil: Bamann, Christian. Max Planck Institute for Biophysics; Alemania | - |
| dc.description | Fil: Wood, Phillip G.. Max Planck Institute for Biophysics; Alemania | - |
| dc.description | Fil: Bamberg, Ernst. Max Planck Institute for Biophysics; Alemania | - |
| dc.description | Fil: Farias, Maria Eugenia. Max Planck Institute for Biophysics; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Planta Piloto de Procesos Industriales Microbiologicos; Argentina | - |
| dc.description | Fil: Gärtner, Wolfgang. Max Planck Institute for Chemical Energy Conversiom; Alemania | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.language | eng | - |
| dc.publisher | Public Library of Science | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1371/journal.pone.0154962 | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0154962 | - |
| dc.rights | info:eu-repo/semantics/openAccess | - |
| dc.rights | https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ | - |
| dc.source | reponame:CONICET Digital (CONICET) | - |
| dc.source | instname:Consejo Nacional de Investigaciones Científicas y Técnicas | - |
| dc.source | instacron:CONICET | - |
| dc.source.uri | http://hdl.handle.net/11336/37774 | - |
| dc.subject | Exiguobacterium | - |
| dc.subject | Stromatolites | - |
| dc.subject | Laguna Socompa | - |
| dc.subject | Irradiation | - |
| dc.subject | Otras Ciencias Biológicas | - |
| dc.subject | Ciencias Biológicas | - |
| dc.subject | CIENCIAS NATURALES Y EXACTAS | - |
| dc.title | Functional green-tuned proteorhodopsin from modern stromatolites | - |
| dc.type | info:eu-repo/semantics/article | - |
| dc.type | info:eu-repo/semantics/publishedVersion | - |
| dc.type | info:ar-repo/semantics/articulo | - |
| Aparece en las colecciones: | CONICET | |
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