Registro completo de metadatos
| Campo DC | Valor | Lengua/Idioma |
|---|---|---|
| dc.provenance | CONICET | - |
| dc.creator | Vichera, Gabriel Damian | - |
| dc.creator | Olivera, Ramiro | - |
| dc.creator | Sipowicz, Pablo | - |
| dc.creator | Radrizzani Helguera, Martin | - |
| dc.creator | Salamone, Daniel Felipe | - |
| dc.date | 2017-05-11T19:36:43Z | - |
| dc.date | 2017-05-11T19:36:43Z | - |
| dc.date | 2011-10 | - |
| dc.date | 2017-05-08T21:18:26Z | - |
| dc.date.accessioned | 2019-04-29T15:39:35Z | - |
| dc.date.available | 2019-04-29T15:39:35Z | - |
| dc.date.issued | 2011-10 | - |
| dc.identifier | Vichera, Gabriel Damian; Olivera, Ramiro; Sipowicz, Pablo; Radrizzani Helguera, Martin; Salamone, Daniel Felipe; Sperm genome cloning used in biparental bovine embryo reconstruction; Csiro Publishing; Reproduction Fertility And Development; 23; 6; 10-2011; 769-779 | - |
| dc.identifier | 1031-3613 | - |
| dc.identifier | http://hdl.handle.net/11336/16331 | - |
| dc.identifier | 1448-5990 | - |
| dc.identifier.uri | http://rodna.bn.gov.ar:8080/jspui/handle/bnmm/298794 | - |
| dc.description | The generation of androgenetic haploid embryos enables several haploid blastomeres to be obtained as identical copies of a single spermatozoon genome. In the present study, we compared the developmental ability of bovine androgenetic haploid embryos constructed by different methods, namely IVF and intracytoplasmic sperm injection (ICSI) before and after oocyte enucleation. Once obtained, the blastomeres of these androgenetic haploid embryos were used as male genome donors to reconstruct biparental embryos by fusion with matured oocytes. To verify the cytoplasmic contribution of androgenetic haploid blastomeres, we used spermatozoa incubated previously with exogenous DNA that coded for a green fluorescent protein gene (pCX-EGFP) and the enhanced green fluorescent protein (EGFP)-positive androgenetic haploid blastomeres generated were fused with mature oocytes. Of the reconstructed embryos reaching the cleavage and blastocyst stages, 85.1% and 9.0%, respectively, expressed EGFP (P . 0.05). EGFP expression was observed in 100% of reconstructed embryos, with 91.2% exhibiting homogenic expression. To confirm sperm genome incorporation, androgenetic haploid blastomeres generated by ICSI prior to enucleation and using Y chromosome sexed spermatozoa were used for biparental embryo reconstruction. Incorporation of the Y chromosome was confirmed by polymerase chain reaction and fluorescence in situ hybridisation analysis. In conclusion, the results of the present study prove that it is possible to use sperm genome replicates to reconstruct biparental bovine embryos and that it is a highly efficient technique to generate homogeneous transgene-expressing embryos. | - |
| dc.description | Fil: Vichera, Gabriel Damian. Universidad de Buenos Aires. Facultad de Agronomía. Pabellón de Zootecnica. Laboratorio de Biotecnología Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | - |
| dc.description | Fil: Olivera, Ramiro. Universidad de Buenos Aires. Facultad de Agronomía. Pabellón de Zootecnica. Laboratorio de Biotecnología Animal; Argentina | - |
| dc.description | Fil: Sipowicz, Pablo. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología. Centro de Estudios en Salud y Medio Ambiente. Laboratorio de Neuro y Citogenética Molecular; Argentina | - |
| dc.description | Fil: Radrizzani Helguera, Martin. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología. Centro de Estudios en Salud y Medio Ambiente. Laboratorio de Neuro y Citogenética Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | - |
| dc.description | Fil: Salamone, Daniel Felipe. Universidad de Buenos Aires. Facultad de Agronomía. Pabellón de Zootecnica. Laboratorio de Biotecnología Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.language | eng | - |
| dc.publisher | Csiro Publishing | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1071/RD10252 | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/url/http://www.publish.csiro.au/rd/RD10252 | - |
| dc.rights | info:eu-repo/semantics/restrictedAccess | - |
| dc.rights | https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ | - |
| dc.source | reponame:CONICET Digital (CONICET) | - |
| dc.source | instname:Consejo Nacional de Investigaciones Científicas y Técnicas | - |
| dc.source | instacron:CONICET | - |
| dc.source.uri | http://hdl.handle.net/11336/16331 | - |
| dc.subject | Sexed haploid NT | - |
| dc.subject | Sperm cloning | - |
| dc.subject | Transgenesis | - |
| dc.subject | Fertility | - |
| dc.subject | Tecnología GM, clonación de ganado, selección asistida, diagnósticos, tecnología de producción de biomasa, etc. | - |
| dc.subject | Biotecnología Agropecuaria | - |
| dc.subject | CIENCIAS AGRÍCOLAS | - |
| dc.title | Sperm genome cloning used in biparental bovine embryo reconstruction | - |
| dc.type | info:eu-repo/semantics/article | - |
| dc.type | info:eu-repo/semantics/publishedVersion | - |
| dc.type | info:ar-repo/semantics/articulo | - |
| Aparece en las colecciones: | CONICET | |
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