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Campo DC | Valor | Lengua/Idioma |
---|---|---|
dc.creator | Lombardo, Veronica Andrea | - |
dc.creator | Otten, Cécile | - |
dc.creator | Abdelilah Seyfried, Salim | - |
dc.date | 2018-06-25T15:46:00Z | - |
dc.date | 2018-06-25T15:46:00Z | - |
dc.date | 2015-01 | - |
dc.date | 2018-06-25T12:39:12Z | - |
dc.date.accessioned | 2019-04-29T15:40:08Z | - |
dc.date.available | 2019-04-29T15:40:08Z | - |
dc.date.issued | 2018-06-25T15:46:00Z | - |
dc.date.issued | 2018-06-25T15:46:00Z | - |
dc.date.issued | 2015-01 | - |
dc.date.issued | 2018-06-25T12:39:12Z | - |
dc.identifier | Lombardo, Veronica Andrea; Otten, Cécile; Abdelilah Seyfried, Salim; Large-scale zebrafish embryonic heart dissection for transcriptional analysis; Journal of Visualized Experiments; Journal of Visualized Experiments; 95; 1-2015; 1-7 | - |
dc.identifier | 1940-087X | - |
dc.identifier | http://hdl.handle.net/11336/49915 | - |
dc.identifier | CONICET Digital | - |
dc.identifier | CONICET | - |
dc.identifier.uri | http://rodna.bn.gov.ar:8080/jspui/handle/bnmm/299026 | - |
dc.description | The zebrafish embryonic heart is composed of only a few hundred cells, representing only a small fraction of the entire embryo. Therefore, to prevent the cardiac transcriptome from being masked by the global embryonic transcriptome, it is necessary to collect sufficient numbers of hearts for further analyses. Furthermore, as zebrafish cardiac development proceeds rapidly, heart collection and RNA extraction methods need to be quick in order to ensure homogeneity of the samples. Here, we present a rapid manual dissection protocol for collecting functional/beating hearts from zebrafish embryos. This is an essential prerequisite for subsequent cardiac-specific RNA extraction to determine cardiac-specific gene expression levels by transcriptome analyses, such as quantitative real-time polymerase chain reaction (RT-qPCR). The method is based on differential adhesive properties of the zebrafish embryonic heart compared with other tissues; this allows for the rapid physical separation of cardiac from extracardiac tissue by a combination of fluidic shear force disruption, stepwise filtration and manual collection of transgenic fluorescently labeled hearts. | - |
dc.description | Fil: Lombardo, Veronica Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Max Delbrück Center for Molecular Medicine; Alemania. Universitat Potsdam; Alemania. Medizinische Hochschule Hannover; Alemania | - |
dc.description | Fil: Otten, Cécile. Universitat Potsdam; Alemania. Max Delbrück Center for Molecular Medicine; Alemania | - |
dc.description | Fil: Abdelilah Seyfried, Salim. Max Delbrück Center for Molecular Medicine; Alemania. Universitat Potsdam; Alemania. Medizinische Hochschule Hannover; Alemania | - |
dc.format | application/pdf | - |
dc.format | application/pdf | - |
dc.language | eng | - |
dc.publisher | Journal of Visualized Experiments | - |
dc.relation | info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.3791/52087 | - |
dc.relation | info:eu-repo/semantics/altIdentifier/url/https://www.jove.com/video/52087/large-scale-zebrafish-embryonic-heart-dissection-for-transcriptional | - |
dc.rights | info:eu-repo/semantics/restrictedAccess | - |
dc.rights | https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ | - |
dc.source | reponame:CONICET Digital (CONICET) | - |
dc.source | instname:Consejo Nacional de Investigaciones Científicas y Técnicas | - |
dc.source | instacron:CONICET | - |
dc.subject | DEVELOPMENTAL BIOLOGY | - |
dc.subject | DISSECTION | - |
dc.subject | EMBRYO | - |
dc.subject | HEART | - |
dc.subject | ISSUE 95 | - |
dc.subject | RNA | - |
dc.subject | RT-QPCR | - |
dc.subject | ZEBRAFISH | - |
dc.subject | Otras Ciencias Biológicas | - |
dc.subject | Ciencias Biológicas | - |
dc.subject | CIENCIAS NATURALES Y EXACTAS | - |
dc.title | Large-scale zebrafish embryonic heart dissection for transcriptional analysis | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.type | info:ar-repo/semantics/articulo | - |
Aparece en las colecciones: | CONICET |
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