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dc.creatorSánchez Miguel, Ignacio Enrique-
dc.creatorDellarole, Mariano-
dc.creatorGaston, Kevin-
dc.creatorde Prat Gay, Gonzalo-
dc.date2017-11-21T17:52:05Z-
dc.date2017-11-21T17:52:05Z-
dc.date2007-12-
dc.date2017-11-16T15:08:05Z-
dc.date.accessioned2019-04-29T15:40:10Z-
dc.date.available2019-04-29T15:40:10Z-
dc.date.issued2017-11-21T17:52:05Z-
dc.date.issued2017-11-21T17:52:05Z-
dc.date.issued2007-12-
dc.date.issued2017-11-16T15:08:05Z-
dc.identifierSánchez Miguel, Ignacio Enrique; Dellarole, Mariano; Gaston, Kevin; de Prat Gay, Gonzalo; Comprehensive comparison of the interaction of the E2 master regulator with its cognate target DNA sites in 73 human papillomavirus types by sequence statistics; Oxford University Press; Nucleic Acids Research; 36; 3; 12-2007; 756-759-
dc.identifier0305-1048-
dc.identifierhttp://hdl.handle.net/11336/28636-
dc.identifier1362-4962-
dc.identifierCONICET Digital-
dc.identifierCONICET-
dc.identifier.urihttp://rodna.bn.gov.ar:8080/jspui/handle/bnmm/299041-
dc.descriptionMucosal human papillomaviruses (HPVs) are etiological agents of oral, anal and genital cancer. Properties of high- and low-risk HPV types cannot be reduced to discrete molecular traits. The E2 protein regulates viral replication and transcription through a finely tuned interaction with four sites at the upstream regulatory region of the genome. A computational study of the E2-DNA interaction in all 73 types within the alpha papillomavirus genus, including all known mucosal types, indicates that E2 proteins have similar DNA discrimination properties. Differences in E2-DNA interaction among HPV types lie mostly in the target DNA sequence, as opposed to the amino acid sequence of the conserved DNA-binding alpha helix of E2. Sequence logos of natural and in vitro selected sites show an asymmetric pattern of conservation arising from indirect readout, and reveal evolutionary pressure for a putative methylation site. Based on DNA sequences only, we could predict differences in binding energies with a standard deviation of 0.64 kcal/mol. These energies cluster into six discrete affinity hierarchies and uncovered a fifth E2-binding site in the genome of six HPV types. Finally, certain distances between sites, affinity hierarchies and their eventual changes upon methylation, are statistically associated with high-risk types-
dc.descriptionFil: Sánchez Miguel, Ignacio Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina-
dc.descriptionFil: Dellarole, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina-
dc.descriptionFil: Gaston, Kevin. University of Bristol; Reino Unido-
dc.descriptionFil: de Prat Gay, Gonzalo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina-
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dc.languageeng-
dc.publisherOxford University Press-
dc.relationinfo:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/nar/article/36/3/756/1377832-
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1093/nar/gkm1104-
dc.rightsinfo:eu-repo/semantics/openAccess-
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/-
dc.sourcereponame:CONICET Digital (CONICET)-
dc.sourceinstname:Consejo Nacional de Investigaciones Científicas y Técnicas-
dc.sourceinstacron:CONICET-
dc.subjecthuman papillomavirus-
dc.subjectprotein DNA interactions-
dc.subjectsequence statistics-
dc.subjectSequence logos-
dc.subjectOtras Ciencias Biológicas-
dc.subjectCiencias Biológicas-
dc.subjectCIENCIAS NATURALES Y EXACTAS-
dc.titleComprehensive comparison of the interaction of the E2 master regulator with its cognate target DNA sites in 73 human papillomavirus types by sequence statistics-
dc.typeinfo:eu-repo/semantics/article-
dc.typeinfo:eu-repo/semantics/publishedVersion-
dc.typeinfo:ar-repo/semantics/articulo-
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