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dc.creatorRoger, Magali-
dc.creatorSciara, Giuliano-
dc.creatorBiaso, Frédéric-
dc.creatorLojou, Elisabeth-
dc.creatorWang, Xie-
dc.creatorBauzan, Marielle-
dc.creatorGiudici Orticoni, Marie-Thérèse-
dc.creatorVila, Alejandro Jose-
dc.creatorIlbert, Marianne-
dc.date2018-12-13T19:03:36Z-
dc.date2018-12-13T19:03:36Z-
dc.date2017-05-
dc.date2018-10-23T16:36:03Z-
dc.date.accessioned2019-04-29T15:41:02Z-
dc.date.available2019-04-29T15:41:02Z-
dc.date.issued2018-12-13T19:03:36Z-
dc.date.issued2018-12-13T19:03:36Z-
dc.date.issued2017-05-
dc.date.issued2018-10-23T16:36:03Z-
dc.identifierRoger, Magali; Sciara, Giuliano; Biaso, Frédéric; Lojou, Elisabeth; Wang, Xie; et al.; Impact of copper ligand mutations on a cupredoxin with a green copper center; Elsevier Science; Biochimica Et Biophysica Acta-bioenergetics; 1858; 5; 5-2017; 351-359-
dc.identifier0005-2728-
dc.identifierhttp://hdl.handle.net/11336/66444-
dc.identifierCONICET Digital-
dc.identifierCONICET-
dc.identifier.urihttp://rodna.bn.gov.ar:8080/jspui/handle/bnmm/299437-
dc.descriptionMononuclear cupredoxins contain a type 1 copper center with a trigonal or tetragonal geometry usually maintained by four ligands, a cystein, two histidines and a methionine. The recent discovery of new members of this family with unusual properties demonstrates, however, the versatility of this class of proteins. Changes in their ligand set lead to drastic variation in their metal site geometry and in the resulting spectroscopic and redox features. In our work, we report the identification of the copper ligands in the recently discovered cupredoxin AcoP. We show that even though AcoP possesses a classical copper ligand set, it has a highly perturbed copper center. In depth studies of mutant's properties suggest a high degree of constraint existing in the copper center of the wild type protein and even the addition of exogenous ligands does not lead to the reconstitution of the initial copper center. Not only the chemical nature of the axial ligand but also constraints brought by its covalent binding to the protein backbone might be critical to maintain a green copper site with high redox potential. This work illustrates the importance of experimentally dissecting the molecular diversity of cupredoxins to determine the molecular determinants responsible for their copper center geometry and redox potential.-
dc.descriptionFil: Roger, Magali. Aix Marseille Univ; Francia-
dc.descriptionFil: Sciara, Giuliano. Aix Marseille Univ; Francia-
dc.descriptionFil: Biaso, Frédéric. Aix Marseille Univ; Francia-
dc.descriptionFil: Lojou, Elisabeth. Aix Marseille Univ; Francia-
dc.descriptionFil: Wang, Xie. Aix Marseille Univ; Francia-
dc.descriptionFil: Bauzan, Marielle. Aix Marseille Univ; Francia-
dc.descriptionFil: Giudici Orticoni, Marie-Thérèse. Aix Marseille Univ; Francia-
dc.descriptionFil: Vila, Alejandro Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Química Biológica. Área Biofísica; Argentina-
dc.descriptionFil: Ilbert, Marianne. Aix Marseille Univ; Francia-
dc.formatapplication/pdf-
dc.formatapplication/pdf-
dc.languageeng-
dc.publisherElsevier Science-
dc.relationinfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0005272817300294-
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1016/j.bbabio.2017.02.007-
dc.rightsinfo:eu-repo/semantics/restrictedAccess-
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/-
dc.sourcereponame:CONICET Digital (CONICET)-
dc.sourceinstname:Consejo Nacional de Investigaciones Científicas y Técnicas-
dc.sourceinstacron:CONICET-
dc.subjectACIDITHIOBACILLUS FERROOXIDANS-
dc.subjectCOPPER-
dc.subjectCUPREDOXIN-
dc.subjectGREEN COPPER SITE-
dc.subjectMETAL CENTER-
dc.subjectMETALLOPROTEIN-
dc.subjectBioquímica y Biología Molecular-
dc.subjectCiencias Biológicas-
dc.subjectCIENCIAS NATURALES Y EXACTAS-
dc.titleImpact of copper ligand mutations on a cupredoxin with a green copper center-
dc.typeinfo:eu-repo/semantics/article-
dc.typeinfo:eu-repo/semantics/publishedVersion-
dc.typeinfo:ar-repo/semantics/articulo-
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