Registro completo de metadatos
| Campo DC | Valor | Lengua/Idioma |
|---|---|---|
| dc.provenance | CONICET | - |
| dc.creator | Rosales, Eliana Paola | - |
| dc.creator | Iannone, María Florencia | - |
| dc.creator | Groppa, María Daniela | - |
| dc.creator | Benavides, Maria Patricia | - |
| dc.date | 2017-06-14T18:50:55Z | - |
| dc.date | 2017-06-14T18:50:55Z | - |
| dc.date | 2011-02 | - |
| dc.date | 2017-06-14T14:35:04Z | - |
| dc.date.accessioned | 2019-04-29T15:41:18Z | - |
| dc.date.available | 2019-04-29T15:41:18Z | - |
| dc.date.issued | 2017-06-14T18:50:55Z | - |
| dc.date.issued | 2017-06-14T18:50:55Z | - |
| dc.date.issued | 2011-02 | - |
| dc.date.issued | 2017-06-14T14:35:04Z | - |
| dc.identifier | Rosales, Eliana Paola; Iannone, María Florencia; Groppa, María Daniela; Benavides, Maria Patricia; Nitric oxide inhibits nitrate reductase activity in wheat leaves; Elsevier Masson; Plant Physiology And Biochemistry; 49; 2; 2-2011; 124-130 | - |
| dc.identifier | 0981-9428 | - |
| dc.identifier | http://hdl.handle.net/11336/18184 | - |
| dc.identifier | CONICET Digital | - |
| dc.identifier | CONICET | - |
| dc.identifier.uri | http://rodna.bn.gov.ar:8080/jspui/handle/bnmm/299558 | - |
| dc.description | Nitrate reductase (NR), a committed enzyme in nitrate assimilation, is involved in the generation of nitric oxide (NO) in plants. In wheat leaf segments exposed to sodium nitroprusside (SNP) or S-nitrosoglutathione (GSNO), NR activity was significantly reduced to different degrees between 3 and 21 h, whereas its activity was partially recovered when the NO scavenger cPTIO was used. At 21 h, NR activity decreased from 38% with 10 μM SNP to 91% with 500 μM SNP, respect to the C values. S-nitrosoglutathione reduced NR activity between 18% and 26% only at 3 h. When added directly to the incubation solution, NR activity was quickly and strongly inhibited more than 90% by 10 or 50 μM SNP, whereas 10 μM GSNO reduced the enzyme activity an average of 50%, at 30 min of incubation. l-NAME and d-arginine (nitric oxide synthase (NOS) inhibitors) increased NR activity by 14% and 52% respectively, at 21 h of exposure, leading us to suppose that endogenous NOS-dependent NO formation could also be modulating NR activity. NR protein expression was not affected by 10 or 100 μM SNP at 3 or 21 h of incubation, whereas nitration of tyrosines was not detected in the NR protein. Nitrates, which content increased along the time in the tissues, could be exerting a role in this regulation. | - |
| dc.description | Fil: Rosales, Eliana Paola. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | - |
| dc.description | Fil: Iannone, María Florencia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | - |
| dc.description | Fil: Groppa, María Daniela. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | - |
| dc.description | Fil: Benavides, Maria Patricia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.language | eng | - |
| dc.publisher | Elsevier Masson | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0981942810002366 | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.plaphy.2010.10.009 | - |
| dc.rights | info:eu-repo/semantics/restrictedAccess | - |
| dc.rights | https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ | - |
| dc.source | reponame:CONICET Digital (CONICET) | - |
| dc.source | instname:Consejo Nacional de Investigaciones Científicas y Técnicas | - |
| dc.source | instacron:CONICET | - |
| dc.source.uri | 1 | - |
| dc.subject | NITRATE REDUCTASE | - |
| dc.subject | NITRIC OXIDE | - |
| dc.subject | NITROGEN METABOLISM | - |
| dc.subject | NITRATES | - |
| dc.subject | TRITICUM AESTIVUM L | - |
| dc.subject | Bioquímica y Biología Molecular | - |
| dc.subject | Ciencias Biológicas | - |
| dc.subject | CIENCIAS NATURALES Y EXACTAS | - |
| dc.title | Nitric oxide inhibits nitrate reductase activity in wheat leaves | - |
| dc.type | info:eu-repo/semantics/article | - |
| dc.type | info:eu-repo/semantics/publishedVersion | - |
| dc.type | info:ar-repo/semantics/articulo | - |
| Aparece en las colecciones: | CONICET | |
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