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dc.creatorVintiñi, Elisa Ofelia-
dc.creatorMedina, Marcela Susana-
dc.date2018-12-20T14:12:45Z-
dc.date2018-12-20T14:12:45Z-
dc.date2017-12-
dc.date2018-12-05T14:46:16Z-
dc.date.accessioned2019-04-29T15:41:53Z-
dc.date.available2019-04-29T15:41:53Z-
dc.date.issued2017-12-
dc.identifierVintiñi, Elisa Ofelia; Medina, Marcela Susana; Non-Viable Lactobacillus Casei Beneficially Modulates Poly I:C Immune Response in Co-Cultures of Human Cells; Shiraz Inst Cancer Res; Iranian Journal Of Immunology; 14; 4; 12-2017; 325-339-
dc.identifier1735-1383-
dc.identifierhttp://hdl.handle.net/11336/66817-
dc.identifier1735-367X-
dc.identifierCONICET Digital-
dc.identifierCONICET-
dc.identifier.urihttp://rodna.bn.gov.ar:8080/jspui/handle/bnmm/299813-
dc.descriptionBackground: Polyinosinic:polycytidylic acid (Poly-IC) has been used as a viralstimulus to mimic in vivo and in vitro infection induced by some viruses. Objective: To determine whether non-viable Lactobacillus casei CRL431 (LcM) can modulate the immune response induced by Poly I:C in co-culture models of peripheral blood mononuclear cells (PBMC) and A549 cells. Methods: T and NK cell activation was evaluated by flow cytometry and levels of TNF-α, IFN-γ, IL-10, IL-29, and IL-17 by ELISA. Cells in direct contact with A549 (PBMC-A549) and cells with no contact with it (PBMC//A549) were used for this purpose. PBMCs alone and both co-culture systems were stimulated for 24 h with the following stimuli: LPS (10 µg/ml), LcM (106 UFC/ml), Poly I:C (2 µg/ml), Poly I:C+LcM, and LcM (3 h)+Poly I:C. Moreover, unstimulated cells were used as a control. Results: Poly I:C and LcM (3 h)+Poly I:C in PBMC-A549 showed a significant increase in the percentage of CD8+ expression (p<0.05). All stimuli induced significant activation from T CD4+, CD8+ cells compared with unstimulated PBMCs in both co-culture cells system. However, activation percentages were higher in direct co-culture. Poly I:C induced a higher level of proinflammatoryTNF-α and IFN-γ cytokines as well as IL-17 and IL-29 with lower IL-10levels in both co-culture systems while LcM induced a beneficial pattern of cytokines that would regulate Poly I:C effect. Conclusion: This in vitro model allowed us to highlight the potential of LcM as a modulator of anti-viral immune response and suggest its potential use in formulations against RNA respiratory viruses.-
dc.descriptionFil: Vintiñi, Elisa Ofelia. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; Argentina-
dc.descriptionFil: Medina, Marcela Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina-
dc.formatapplication/pdf-
dc.formatapplication/pdf-
dc.languageeng-
dc.publisherShiraz Inst Cancer Res-
dc.relationinfo:eu-repo/semantics/altIdentifier/url/http://iji.sums.ac.ir/article_39328_f12863523edece69a6bfabf6e8c2692e.pdf-
dc.rightsinfo:eu-repo/semantics/openAccess-
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/-
dc.sourcereponame:CONICET Digital (CONICET)-
dc.sourceinstname:Consejo Nacional de Investigaciones Científicas y Técnicas-
dc.sourceinstacron:CONICET-
dc.source.urihttp://hdl.handle.net/11336/65252-
dc.subjectPoly I.C-
dc.subjectANTIVIRAL IMMUNE RESPONSE-
dc.subjectNON VIABLE LACTOBACILLUS-
dc.subjectPBMC/A549-
dc.subjectSalud Ocupacional-
dc.subjectCiencias de la Salud-
dc.subjectCIENCIAS MÉDICAS Y DE LA SALUD-
dc.titleNon-Viable Lactobacillus Casei Beneficially Modulates Poly I:C Immune Response in Co-Cultures of Human Cells-
dc.typeinfo:eu-repo/semantics/article-
dc.typeinfo:eu-repo/semantics/publishedVersion-
dc.typeinfo:ar-repo/semantics/articulo-
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