Registro completo de metadatos
| Campo DC | Valor | Lengua/Idioma |
|---|---|---|
| dc.creator | Diosque, Patricio | - |
| dc.creator | Tomasini, Nicolás | - |
| dc.creator | Lauthier, Juan José | - |
| dc.creator | Messenger, Louisa Alexandra | - |
| dc.creator | Monje Rumi, Maria Mercedes | - |
| dc.creator | Ragone, Paula Gabriela | - |
| dc.creator | Alberti D'Amato, Anahi Maiten | - |
| dc.creator | Perez Brandan, Cecilia Maria | - |
| dc.creator | Barnabe, Christian | - |
| dc.creator | Tibayrenc, Michel | - |
| dc.creator | Lewis, Michael David | - |
| dc.creator | Llewellyn, Martin Stephen | - |
| dc.creator | Miles, Michael Alexander | - |
| dc.creator | Yeo, Matthew | - |
| dc.date | 2016-08-11T14:25:16Z | - |
| dc.date | 2016-08-11T14:25:16Z | - |
| dc.date | 2014-08-28 | - |
| dc.date | 2016-08-04T18:17:41Z | - |
| dc.date.accessioned | 2019-04-29T15:42:10Z | - |
| dc.date.available | 2019-04-29T15:42:10Z | - |
| dc.date.issued | 2014-08-28 | - |
| dc.identifier | Diosque, Patricio; Tomasini, Nicolás; Lauthier, Juan José; Messenger, Louisa Alexandra; Monje Rumi, Maria Mercedes; et al.; Optimized multilocus sequence typing (MLST) scheme for Trypanosoma cruzi.; Public Library Of Science; Neglected Tropical Diseases; 8; 8; 28-8-2014; e3117 | - |
| dc.identifier | 1935-2735 | - |
| dc.identifier | http://hdl.handle.net/11336/7087 | - |
| dc.identifier.uri | http://rodna.bn.gov.ar:8080/jspui/handle/bnmm/299932 | - |
| dc.description | Trypanosoma cruzi, the aetiological agent of Chagas disease possess extensive genetic diversity. This has led to the development of a plethora of molecular typing methods for the identification of both the known major genetic lineages and for more fine scale characterization of different multilocus genotypes within these major lineages. Whole genome sequencing applied to large sample sizes is not currently viable and multilocus enzyme electrophoresis, the previous gold standard for T. cruzi typing, is laborious and time consuming. In the present work, we present an optimized Multilocus Sequence Typing (MLST) scheme, based on the combined analysis of two recently proposed MLST approaches. Here,thirteen concatenated gene fragments were applied to a panel of T. cruzi reference strains encompassing all known genetic lineages. Concatenation of 13 fragments allowed assignment of all strains to the predicted Discrete Typing Units (DTUs), or near-clades, with the exception of one strain that was an outlier for TcV, due to apparent loss of heterozygosity in one fragment. Monophyly for all DTUs, along with robust bootstrap support, was restored when this fragment was subsequently excluded from the analysis. All possible combinations of loci were assessed against predefined criteria with the objective of selecting the most appropriate combination of between two and twelve fragments, for an optimized MLST scheme. The optimum combination consisted of 7 loci and discriminated between all reference strains in the panel, with the majority supported by robust bootstrap values. Additionally, a reduced panel of just 4 gene fragments displayed high bootstrap values for DTU assignment and discriminated 21 out of 25 genotypes. We propose that the seven-fragment MLST scheme could be used as a gold standard for T. cruzi typing, against which other typing approaches, particularly single locus approaches or systematic PCR assays based on amplicon size, could be compared. | - |
| dc.description | Fil: Diosque, Patricio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Patología Experimental; Argentina. Universidad Nacional de Salta; Argentina | - |
| dc.description | Fil: Tomasini, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Patología Experimental; Argentina. Universidad Nacional de Salta; Argentina | - |
| dc.description | Fil: Lauthier, Juan José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Patología Experimental; Argentina. Universidad Nacional de Salta; Argentina | - |
| dc.description | Fil: Messenger, Louisa Alexandra. London School of Hygiene and Tropical Medicine; Reino Unido | - |
| dc.description | Fil: Monje Rumi, Maria Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Patología Experimental; Argentina. Universidad Nacional de Salta; Argentina | - |
| dc.description | Fil: Ragone, Paula Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Patología Experimental; Argentina. Universidad Nacional de Salta; Argentina | - |
| dc.description | Fil: Alberti D'Amato, Anahi Maiten. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Patología Experimental; Argentina. Universidad Nacional de Salta; Argentina | - |
| dc.description | Fil: Perez Brandan, Cecilia Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Patología Experimental; Argentina. Universidad Nacional de Salta; Argentina | - |
| dc.description | Fil: Barnabe, Christian. Institut de Recherche pour le Développement. Maladies Infectieuses et Vecteurs Ecologie, Génétique, Evolution et Contrôle; Francia | - |
| dc.description | Fil: Tibayrenc, Michel. Institut de Recherche our le Développement. Maladies Infectieuses et Vecteurs Ecologie, Génétique, Evolution et Contrôle; Francia | - |
| dc.description | Fil: Lewis, Michael David. London School of Hygiene and Tropical Medicine; Reino Unido | - |
| dc.description | Fil: Llewellyn, Martin Stephen. London School of Hygiene and Tropical Medicine; Reino Unido | - |
| dc.description | Fil: Miles, Michael Alexander. London School of Hygiene and Tropical Medicine; Reino Unido | - |
| dc.description | Fil: Yeo, Matthew. London School of Hygiene and Tropical Medicine; Reino Unido | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.language | eng | - |
| dc.publisher | Public Library Of Science | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pntd.0003117 | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1371/journal.pntd.0003117 | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0003117 | - |
| dc.rights | info:eu-repo/semantics/openAccess | - |
| dc.rights | https://creativecommons.org/licenses/by/2.5/ar/ | - |
| dc.source | reponame:CONICET Digital (CONICET) | - |
| dc.source | instname:Consejo Nacional de Investigaciones Científicas y Técnicas | - |
| dc.source | instacron:CONICET | - |
| dc.source.uri | http://hdl.handle.net/11336/69254 | - |
| dc.subject | MULTILOCUS SEQUENCE TYPING | - |
| dc.subject | TRYPANOSOMA CRUZI | - |
| dc.subject | SCHEME OF OPTIMIZATION | - |
| dc.subject | GENETIC DIVERSITY | - |
| dc.subject | Enfermedades Infecciosas | - |
| dc.subject | Ciencias de la Salud | - |
| dc.subject | CIENCIAS MÉDICAS Y DE LA SALUD | - |
| dc.title | Optimized multilocus sequence typing (MLST) scheme for Trypanosoma cruzi. | - |
| dc.type | info:eu-repo/semantics/article | - |
| dc.type | info:eu-repo/semantics/publishedVersion | - |
| dc.type | info:ar-repo/semantics/articulo | - |
| Aparece en las colecciones: | CONICET | |
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