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dc.creatorDomenech, Rosa-
dc.creatorHernández Cifre, José G.-
dc.creatorBacarizo, Julio-
dc.creatorDiez Peña, Ana I.-
dc.creatorMartínez Rodríguez, Sergio-
dc.creatorCavasotto, Claudio Norberto-
dc.creatorGarcía de la Torre, José-
dc.creatorCámara Artigás, Ana-
dc.creatorVelázquez Campoy, Adrián-
dc.creatorNeira, José L.-
dc.date2017-10-24T14:10:44Z-
dc.date2017-10-24T14:10:44Z-
dc.date2013-07-
dc.date2017-10-06T18:57:04Z-
dc.date.accessioned2019-04-29T15:42:25Z-
dc.date.available2019-04-29T15:42:25Z-
dc.date.issued2013-07-
dc.identifierDomenech, Rosa; Hernández Cifre, José G.; Bacarizo, Julio; Diez Peña, Ana I.; Martínez Rodríguez, Sergio; et al.; The Histidine-Phosphocarrier protein of the Phosphoenolpyruvate: sugar Phosphotransferase system of Bacillus sphaericus self-associates; Public Library of Science; Plos One; 8; 7; 7-2013; 1-15; e69307-
dc.identifier1932-6203-
dc.identifierhttp://hdl.handle.net/11336/26998-
dc.identifierCONICET Digital-
dc.identifierCONICET-
dc.identifier.urihttp://rodna.bn.gov.ar:8080/jspui/handle/bnmm/300030-
dc.descriptionThe phosphotransferase system (PTS) is involved in the use of carbon sources in bacteria. Bacillus sphaericus, a bacterium with the ability to produce insecticidal proteins, is unable to use hexoses and pentoses as the sole carbon source, but it has ptsHI genes encoding the two general proteins of the PTS: enzyme I (EI) and the histidine phosphocarrier (HPr). In this work, we describe the biophysical and structural properties of HPr from B. sphaericus, HPrbs, and its affinity towards EI of other species to find out whether there is inter-species binding. Conversely to what happens to other members of the HPr family, HPrbs forms several self-associated species. The conformational stability of the protein is low, and it unfolds irreversibly during heating. The protein binds to the N-terminal domain of EI from Streptomyces coelicolor, EINsc, with a higher affinity than that of the natural partner of EINsc, HPrsc. Modelling of the complex between EINsc and HPrbs suggests that binding occurs similarly to that observed in other HPr species. We discuss the functional implications of the oligomeric states of HPrbs for the glycolytic activity of B. sphaericus, as well as a strategy to inhibit binding between HPrsc and EINsc.-
dc.descriptionFil: Domenech, Rosa. Universidad de Miguel Hernández; España-
dc.descriptionFil: Hernández Cifre, José G.. Universidad de Murcia; España-
dc.descriptionFil: Bacarizo, Julio. Universidad de Almería; España-
dc.descriptionFil: Diez Peña, Ana I.. Universidad de Murcia; España-
dc.descriptionFil: Martínez Rodríguez, Sergio. Universidad de Miguel Hernández; España. Universidad de Almería; España-
dc.descriptionFil: Cavasotto, Claudio Norberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación en Biomedicina de Buenos Aires - Instituto Partner de la Sociedad Max Planck; Argentina-
dc.descriptionFil: García de la Torre, José. Universidad de Murcia; España-
dc.descriptionFil: Cámara Artigás, Ana. Universidad de Almería; España-
dc.descriptionFil: Velázquez Campoy, Adrián. Universidad de Zaragoza; España-
dc.descriptionFil: Neira, José L.. Universidad de Miguel Hernández; España-
dc.formatapplication/pdf-
dc.formatapplication/pdf-
dc.languageeng-
dc.publisherPublic Library of Science-
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1371/journal.pone.0069307-
dc.relationinfo:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0069307-
dc.rightsinfo:eu-repo/semantics/openAccess-
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/-
dc.sourcereponame:CONICET Digital (CONICET)-
dc.sourceinstname:Consejo Nacional de Investigaciones Científicas y Técnicas-
dc.sourceinstacron:CONICET-
dc.subjectHISTIDINE-PHOSPHOCARRIER PROTEIN-
dc.subjectBACILUS SPHAERICUS-
dc.subjectPROTEIN-PROTEIN INTERACTION-
dc.subjectENZYME I-
dc.subjectBioquímica y Biología Molecular-
dc.subjectCiencias Biológicas-
dc.subjectCIENCIAS NATURALES Y EXACTAS-
dc.titleThe Histidine-Phosphocarrier protein of the Phosphoenolpyruvate: sugar Phosphotransferase system of Bacillus sphaericus self-associates-
dc.typeinfo:eu-repo/semantics/article-
dc.typeinfo:eu-repo/semantics/publishedVersion-
dc.typeinfo:ar-repo/semantics/articulo-
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