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dc.creatorHebert, Elvira Maria-
dc.creatorSavoy, Graciela-
dc.creatorRaya, Raul Ricardo-
dc.date2018-03-06T21:14:57Z-
dc.date2018-03-06T21:14:57Z-
dc.date2001-04-
dc.date2018-03-06T17:03:56Z-
dc.date.accessioned2019-04-29T15:43:24Z-
dc.date.available2019-04-29T15:43:24Z-
dc.date.issued2001-04-
dc.identifierHebert, Elvira Maria; Savoy, Graciela; Raya, Raul Ricardo; Isolation and Characterization of a Slowly Milk-Coagulating Variant of Lactobacillus helveticus Deficient in Purine Biosynthesis; American Society for Microbiology; Applied And Environmental Microbiology; 67; 4; 4-2001; 1846-1850-
dc.identifier0099-2240-
dc.identifierhttp://hdl.handle.net/11336/38095-
dc.identifierCONICET Digital-
dc.identifierCONICET-
dc.identifier.urihttp://rodna.bn.gov.ar:8080/jspui/handle/bnmm/300340-
dc.descriptionA slowly milk-coagulating variant (Fmc-) of Lactobacillus helveticus CRL 1062, designated S1, was isolated and characterized. Strain S1 possessed all the known essential components required to utilize casein as a nitrogen source, which include functional proteinase and peptidase activities as well as functional amino acid, di- and tripeptide, and oligopeptide transport systems. The amino acid requirements of strain S1 were similar to those of the parental strain. However, on a purine-free, chemically defined medium, the growth rate of the Fmc- strain was threefold lower than that of the wild-type strain. L. helveticus S1 was found to be defective in IMP dehydrogenase activity and therefore was deficient in the ability to synthesize XMP and GMP. This conclusion was further supported by the observation that the addition of guanine or xanthine to milk, a substrate poor in purine compounds, restored the Fmc+ phenotype of L. helveticus S1.-
dc.descriptionFil: Hebert, Elvira Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina-
dc.descriptionFil: Savoy, Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina-
dc.descriptionFil: Raya, Raul Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina-
dc.formatapplication/pdf-
dc.formatapplication/pdf-
dc.formatapplication/pdf-
dc.formatapplication/pdf-
dc.languageeng-
dc.publisherAmerican Society for Microbiology-
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1128/AEM.67.4.1846-1850.2001-
dc.relationinfo:eu-repo/semantics/altIdentifier/url/http://aem.asm.org/content/67/4/1846.full-
dc.rightsinfo:eu-repo/semantics/restrictedAccess-
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/-
dc.sourcereponame:CONICET Digital (CONICET)-
dc.sourceinstname:Consejo Nacional de Investigaciones Científicas y Técnicas-
dc.sourceinstacron:CONICET-
dc.subjectSlowly milk-coagulating variant-
dc.subjectLactobacillus helveticus-
dc.subjectPurine-
dc.subjectBiología Celular, Microbiología-
dc.subjectCiencias Biológicas-
dc.subjectCIENCIAS NATURALES Y EXACTAS-
dc.titleIsolation and Characterization of a Slowly Milk-Coagulating Variant of Lactobacillus helveticus Deficient in Purine Biosynthesis-
dc.typeinfo:eu-repo/semantics/article-
dc.typeinfo:eu-repo/semantics/publishedVersion-
dc.typeinfo:ar-repo/semantics/articulo-
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