Registro completo de metadatos
| Campo DC | Valor | Lengua/Idioma |
|---|---|---|
| dc.creator | Girard Gagnepain, Anais | - |
| dc.creator | Amirache, Fouzia | - |
| dc.creator | Costa, Caroline | - |
| dc.creator | Lévy, Camille | - |
| dc.creator | Frecha, Cecilia Ariana | - |
| dc.creator | Fusil, Floriane | - |
| dc.creator | Nègre, Didier | - |
| dc.creator | Lavillette, Dimitri | - |
| dc.creator | Cosset, François-Loïc | - |
| dc.creator | Verhoeyen, Els | - |
| dc.date | 2017-06-06T18:37:02Z | - |
| dc.date | 2017-06-06T18:37:02Z | - |
| dc.date | 2014-08 | - |
| dc.date | 2017-05-26T14:42:17Z | - |
| dc.date.accessioned | 2019-04-29T15:43:30Z | - |
| dc.date.available | 2019-04-29T15:43:30Z | - |
| dc.date.issued | 2017-06-06T18:37:02Z | - |
| dc.date.issued | 2017-06-06T18:37:02Z | - |
| dc.date.issued | 2014-08 | - |
| dc.date.issued | 2017-05-26T14:42:17Z | - |
| dc.identifier | Girard Gagnepain, Anais; Amirache, Fouzia; Costa, Caroline; Lévy, Camille; Frecha, Cecilia Ariana; et al.; Baboon envelope pseudotyped LVs outperform VSV-G-LVs for gene transfer into early-cytokine-stimulated and resting HSCs; American Society of Hematology; Blood, The Journal Of The American Society Of Hematology - Print; 124; 8; 8-2014; 1221-1231 | - |
| dc.identifier | 0006-4971 | - |
| dc.identifier | http://hdl.handle.net/11336/17590 | - |
| dc.identifier.uri | http://rodna.bn.gov.ar:8080/jspui/handle/bnmm/300380 | - |
| dc.description | Hematopoietic stem cell (HSC)-based gene therapy holds promise for the cure of many diseases. The field is now moving toward the use of lentiviral vectors (LVs) as evidenced by 4 successful clinical trials. These trials used vesicular-stomatitis-virus-G protein (VSV-G)-LVs at high doses combined with strong cytokine-cocktail stimulation to obtain therapeutically relevant transduction levels; however, they might compromise the HSC character. Summarizing all these disadvantages, alternatives to VSV-G-LVs are urgently needed. We generated here high-titer LVs pseudotyped with a baboon retroviral envelope glycoprotein (BaEV-LVs), resistant to human complement. Under mild cytokine prestimulation to preserve the HSC characteristics, a single BaEV-LV application at a low dose, resulted in up to 90% of hCD34+ cell transduction. Even more striking was that these new BaEV-LVs allowed, at low doses, efficient transduction of up to 30% of quiescent hCD34+ cells, whereas high-dose VSV-G-LVs were insufficient. Importantly, reconstitution of NOD/Lt-SCID/γc−/− (NSG) mice with BaEV-LV-transduced hCD34+ cells maintained these high transduction levels in all myeloid and lymphoid lineages, including early progenitors. This transduction pattern was confirmed or even increased in secondary NSG recipient mice. This suggests that BaEV-LVs efficiently transduce true HSCs and could improve HSC-based gene therapy, for which high-level HSC correction is needed for life-long cure. | - |
| dc.description | Fil: Girard Gagnepain, Anais. Université Claude Bernard Lyon 1; Francia | - |
| dc.description | Fil: Amirache, Fouzia. Université Claude Bernard Lyon 1; Francia | - |
| dc.description | Fil: Costa, Caroline. Université Claude Bernard Lyon 1; Francia | - |
| dc.description | Fil: Lévy, Camille. Université Claude Bernard Lyon 1; Francia | - |
| dc.description | Fil: Frecha, Cecilia Ariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencias; Argentina | - |
| dc.description | Fil: Fusil, Floriane. Université Claude Bernard Lyon 1; Francia | - |
| dc.description | Fil: Nègre, Didier. Université Claude Bernard Lyon 1; Francia | - |
| dc.description | Fil: Lavillette, Dimitri. Centre National de la Recherche Scientifique; Francia | - |
| dc.description | Fil: Cosset, François-Loïc. Université Claude Bernard Lyon 1; Francia | - |
| dc.description | Fil: Verhoeyen, Els. Université Claude Bernard Lyon 1; Francia. Inserm; Francia | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.language | eng | - |
| dc.publisher | American Society of Hematology | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/url/http://www.bloodjournal.org/content/124/8/1221.long?sso-checked=true | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1182/blood-2014-02-558163 | - |
| dc.rights | info:eu-repo/semantics/restrictedAccess | - |
| dc.rights | https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ | - |
| dc.source | reponame:CONICET Digital (CONICET) | - |
| dc.source | instname:Consejo Nacional de Investigaciones Científicas y Técnicas | - |
| dc.source | instacron:CONICET | - |
| dc.subject | Hematopoietic Stem Cells gene therapy | - |
| dc.subject | Baboon envelope | - |
| dc.subject | Lentiviral vectors | - |
| dc.subject | Otras Biotecnologías de la Salud | - |
| dc.subject | Biotecnología de la Salud | - |
| dc.subject | CIENCIAS MÉDICAS Y DE LA SALUD | - |
| dc.title | Baboon envelope pseudotyped LVs outperform VSV-G-LVs for gene transfer into early-cytokine-stimulated and resting HSCs | - |
| dc.type | info:eu-repo/semantics/article | - |
| dc.type | info:eu-repo/semantics/publishedVersion | - |
| dc.type | info:ar-repo/semantics/articulo | - |
| Aparece en las colecciones: | CONICET | |
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