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Campo DC | Valor | Lengua/Idioma |
---|---|---|
dc.creator | Danielsson, Jens | - |
dc.creator | Mu, Xin | - |
dc.creator | Lang, Lisa | - |
dc.creator | Wang, Huabing | - |
dc.creator | Binolfi, Andrés | - |
dc.creator | Theillet, François Xavier | - |
dc.creator | Bekei, Beata | - |
dc.creator | Logan, Derek T. | - |
dc.creator | Selenko, Philipp | - |
dc.creator | Wennerström, Håkan | - |
dc.creator | Oliveberg, Mikael | - |
dc.date | 2018-07-18T17:55:31Z | - |
dc.date | 2018-07-18T17:55:31Z | - |
dc.date | 2015-10 | - |
dc.date | 2018-07-17T13:55:28Z | - |
dc.date.accessioned | 2019-04-29T15:43:32Z | - |
dc.date.available | 2019-04-29T15:43:32Z | - |
dc.date.issued | 2018-07-18T17:55:31Z | - |
dc.date.issued | 2018-07-18T17:55:31Z | - |
dc.date.issued | 2015-10 | - |
dc.date.issued | 2018-07-17T13:55:28Z | - |
dc.identifier | Danielsson, Jens; Mu, Xin; Lang, Lisa; Wang, Huabing; Binolfi, Andrés; et al.; Thermodynamics of protein destabilization in live cells; National Academy of Sciences; Proceedings of the National Academy of Sciences of The United States of America; 112; 40; 10-2015; 12402-12407 | - |
dc.identifier | 0027-8424 | - |
dc.identifier | http://hdl.handle.net/11336/52589 | - |
dc.identifier | CONICET Digital | - |
dc.identifier | CONICET | - |
dc.identifier.uri | http://rodna.bn.gov.ar:8080/jspui/handle/bnmm/300398 | - |
dc.description | Although protein folding and stability have been well explored under simplified conditions in vitro, it is yet unclear how these basic self-organization events are modulated by the crowded interior of live cells. To find out, we use here in-cell NMR to follow at atomic resolution the thermal unfolding of a ß-barrel protein inside mammalian and bacterial cells. Challenging the view from in vitro crowding effects, we find that the cells destabilize the protein at 37°C but with a conspicuous twist While the melting temperature goes down the cold unfolding moves into the physiological regime, coupled to an augmented heat-capacity change. The effect seems induced by transient, sequence-specific, interactions with the cellular components, acting preferentially on the unfolded ensemble. This points to a model where the in vivo influence on protein behavior is case specific, determined by the individual protein's interplay with the functionally optimized "interaction landscape" of the cellular interior. | - |
dc.description | Fil: Danielsson, Jens. Stockholms Universitet; Suecia | - |
dc.description | Fil: Mu, Xin. Stockholms Universitet; Suecia | - |
dc.description | Fil: Lang, Lisa. Stockholms Universitet; Suecia | - |
dc.description | Fil: Wang, Huabing. Stockholms Universitet; Suecia | - |
dc.description | Fil: Binolfi, Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Leibniz Institute of Molecular Pharmacology; Alemania | - |
dc.description | Fil: Theillet, François Xavier. Leibniz Institute of Molecular Pharmacology; Alemania | - |
dc.description | Fil: Bekei, Beata. Leibniz Institute of Molecular Pharmacology; Alemania | - |
dc.description | Fil: Logan, Derek T.. Lund University; Suecia | - |
dc.description | Fil: Selenko, Philipp. Leibniz Institute of Molecular Pharmacology; Alemania | - |
dc.description | Fil: Wennerström, Håkan. Lund University; Suecia | - |
dc.description | Fil: Oliveberg, Mikael. Stockholms Universitet; Suecia | - |
dc.format | application/pdf | - |
dc.format | application/pdf | - |
dc.language | eng | - |
dc.publisher | National Academy of Sciences | - |
dc.relation | info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1073/pnas.1511308112 | - |
dc.relation | info:eu-repo/semantics/altIdentifier/url/http://www.pnas.org/content/112/40/12402 | - |
dc.rights | info:eu-repo/semantics/openAccess | - |
dc.rights | https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ | - |
dc.source | reponame:CONICET Digital (CONICET) | - |
dc.source | instname:Consejo Nacional de Investigaciones Científicas y Técnicas | - |
dc.source | instacron:CONICET | - |
dc.subject | CROWDING | - |
dc.subject | IN VIVO | - |
dc.subject | NMR | - |
dc.subject | PROTEIN STABILITY | - |
dc.subject | THERMODYNAMICS | - |
dc.subject | Otras Ciencias Biológicas | - |
dc.subject | Ciencias Biológicas | - |
dc.subject | CIENCIAS NATURALES Y EXACTAS | - |
dc.title | Thermodynamics of protein destabilization in live cells | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.type | info:ar-repo/semantics/articulo | - |
Aparece en las colecciones: | CONICET |
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