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dc.provenanceCONICET-
dc.creatorHonegger, A.-
dc.creatorHofer, E.-
dc.creatorBarañao, Rosa Ines-
dc.creatorMackinlay, D-
dc.creatorMackinlay, T.-
dc.creatorBullorsky, E.-
dc.creatorBordenave, R.-
dc.creatorChasseing, Norma Alejandra-
dc.date2017-12-14T21:36:53Z-
dc.date2017-12-14T21:36:53Z-
dc.date2002-
dc.date2017-12-04T17:53:58Z-
dc.date.accessioned2019-04-29T15:44:23Z-
dc.date.available2019-04-29T15:44:23Z-
dc.date.issued2017-12-14T21:36:53Z-
dc.date.issued2017-12-14T21:36:53Z-
dc.date.issued2002-
dc.date.issued2017-12-04T17:53:58Z-
dc.identifierHonegger, A.; Hofer, E.; Barañao, Rosa Ines; Mackinlay, D; Mackinlay, T.; et al.; Interleukin-1 beta, transforming growth factor beta 1, prostaglandin E2, and fibronectin levels in the conditioned mediums of bone marrow fibroblast cultures from lung and breast cancer patients; Springer Verlag Berlín; Annals Of Hematology; 81; 2; 2002; 80-85-
dc.identifier0939-5555-
dc.identifierhttp://hdl.handle.net/11336/30706-
dc.identifier1432-0584-
dc.identifierCONICET Digital-
dc.identifierCONICET-
dc.identifier.urihttp://rodna.bn.gov.ar:8080/jspui/handle/bnmm/300770-
dc.descriptionWe analyzed the ability of the bone marrow (BM) stromal cells to achieve confluence and their proliferative capacity in BM primary cultures from 30 untreated lung cancer patients (LCP), 27 breast cancer patients (BCP), and 30 normal controls (NC) when these confluent cells were induced to proliferate following four continuous subcultures. Moreover, we evaluated the production of interleukin-1 beta (IL-1beta), transforming growth factor beta 1 (TGF-beta1), fibronectin, and prostaglandin E2 (PGE2) by pure fibroblasts (fourth passage). A fibroblast colony-forming units (CFU-F) assay was used to investigate the proliferative and confluence capacity. Levels of IL-1beta, TGF-beta1, and fibronectin in conditioned mediums (CM) of fibroblast cultures were measured by enzyme-linked immunosorbent assay (ELISA) kit and PGE(2) by radioimmunoassay (RIA) kit. Confluence was achieved in the 60% of LCP and 78% of BCP primary cultures compared with 100% of NC, and only fibroblasts from seven LCP and six BCP cultures had the capacity to proliferate following four subcultures. Levels of IL-1beta were below 10 pg/ml in both patient groups, while NC had a mean value of 5882.57+/-221.61 pg/ml. Levels of TGF-beta1 in BCP were lower than NC values ( P<0.05). LCP and BCP had significantly decreased levels of fibronectin when compared to NC values ( P<0.05 and P<0.01, respectively). Levels of PGE2 in LCP were higher compared to NC ( P<0.01). In conclusion, BM fibroblasts from LCP and BCP presented a defective proliferative and confluence capacity, and this deficiency may be associated with the alteration of IL-1beta, TGF-beta1, fibronectin, and PGE2 production.-
dc.descriptionFil: Honegger, A.. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina-
dc.descriptionFil: Hofer, E.. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina-
dc.descriptionFil: Barañao, Rosa Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina-
dc.descriptionFil: Mackinlay, D. Hospital Británico de Buenos Aires; Argentina-
dc.descriptionFil: Mackinlay, T.. Hospital Británico de Buenos Aires. Servicio de Alergia e Inmunología Clínica; Argentina-
dc.descriptionFil: Bullorsky, E.. Hospital Británico de Buenos Aires; Argentina-
dc.descriptionFil: Bordenave, R.. Hospital Zonal General de Agudos Dr. Isidoro G. Iriarte; Argentina-
dc.descriptionFil: Chasseing, Norma Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina-
dc.formatapplication/pdf-
dc.formatapplication/pdf-
dc.languageeng-
dc.publisherSpringer Verlag Berlín-
dc.relationinfo:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs00277-001-0410-y-
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1007/s00277-001-0410-y-
dc.rightsinfo:eu-repo/semantics/restrictedAccess-
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/-
dc.sourcereponame:CONICET Digital (CONICET)-
dc.sourceinstname:Consejo Nacional de Investigaciones Científicas y Técnicas-
dc.sourceinstacron:CONICET-
dc.source.urihttp://hdl.handle.net/11336/30706-
dc.subjectFIBROBLAST-
dc.subjectSOLUBLE RACTORS-
dc.subjectCANCER-
dc.subjectInmunología-
dc.subjectMedicina Básica-
dc.subjectCIENCIAS MÉDICAS Y DE LA SALUD-
dc.titleInterleukin-1 beta, transforming growth factor beta 1, prostaglandin E2, and fibronectin levels in the conditioned mediums of bone marrow fibroblast cultures from lung and breast cancer patients-
dc.typeinfo:eu-repo/semantics/article-
dc.typeinfo:eu-repo/semantics/publishedVersion-
dc.typeinfo:ar-repo/semantics/articulo-
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