Registro completo de metadatos
| Campo DC | Valor | Lengua/Idioma |
|---|---|---|
| dc.creator | Luquita, A. | - |
| dc.creator | Urli, L. | - |
| dc.creator | Svetaz, M. J. | - |
| dc.creator | Gennaro, Ana Maria | - |
| dc.creator | Giorgietti, M. | - |
| dc.creator | Pistone, G. | - |
| dc.creator | Volpintesta, R. | - |
| dc.creator | Palatnik, S. | - |
| dc.creator | Rasia, M. | - |
| dc.date | 2017-03-13T14:16:41Z | - |
| dc.date | 2017-03-13T14:16:41Z | - |
| dc.date | 2010-12 | - |
| dc.date | 2017-02-24T19:29:10Z | - |
| dc.date.accessioned | 2019-04-29T15:45:32Z | - |
| dc.date.available | 2019-04-29T15:45:32Z | - |
| dc.date.issued | 2010-12 | - |
| dc.identifier | Luquita, A.; Urli, L.; Svetaz, M. J.; Gennaro, Ana Maria; Giorgietti, M.; et al.; In vitro and ex vivo effect of hyaluronic acid on erythrocyte flow properties; Biomed Central; Journal Of Biomedical Science; 17; 12-2010; 8-14 | - |
| dc.identifier | 1021-7770 | - |
| dc.identifier | http://hdl.handle.net/11336/13757 | - |
| dc.identifier.uri | http://rodna.bn.gov.ar:8080/jspui/handle/bnmm/301301 | - |
| dc.description | Background: Hyaluronic acid (HA) is present in many tissues; its presence in serum may be related to certaininflammatory conditions, tissue damage, sepsis, liver malfunction and some malignancies. In the present work, ourgoal was to investigate the significance of hyaluronic acid effect on erythrocyte flow properties. Therefore weperformed in vitro experiments incubating red blood cells (RBCs) with several HA concentrations. Afterwards, in orderto corroborate the pathophysiological significance of the results obtained, we replicated the in vitro experiment with exvivo RBCs from diagnosed rheumatoid arthritis (RA) patients, a serum HA-increasing pathology.Methods: Erythrocyte deformability (by filtration through nucleopore membranes) and erythrocyte aggregability (EA)were tested on blood from healthy donors additioned with purified HA. EA was measured by transmitted light andanalyzed with a mathematical model yielding two parameters, the aggregation rate and the size of the aggregates.Conformational changes of cytoskeleton proteins were estimated by electron paramagnetic resonance spectroscopy(EPR).Results: In vitro, erythrocytes treated with HA showed increased rigidity index (RI) and reduced aggregability, situationstrongly related to the rigidization of the membrane cytoskeleton triggered by HA, as shown by EPR results. Also, asignificant correlation (r: 0.77, p Conclusions: Our results lead us to postulate the hypothesis that HA interacts with the erythrocyte surface leading tomodifications in erythrocyte rheological and flow properties, both ex vivo and in vitro. | - |
| dc.description | Fil: Luquita, A.. Universidad Nacional de Rosario; Argentina | - |
| dc.description | Fil: Urli, L.. Universidad Nacional de Rosario; Argentina | - |
| dc.description | Fil: Svetaz, M. J.. Universidad Nacional de Rosario; Argentina | - |
| dc.description | Fil: Gennaro, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química (i); Argentina. Universidad Nacional del Litoral; Argentina | - |
| dc.description | Fil: Giorgietti, M.. Universidad Nacional de Rosario; Argentina | - |
| dc.description | Fil: Pistone, G.. Universidad Nacional de Rosario; Argentina | - |
| dc.description | Fil: Volpintesta, R.. Universidad Nacional de Rosario; Argentina | - |
| dc.description | Fil: Palatnik, S.. Universidad Nacional de Rosario; Argentina | - |
| dc.description | Fil: Rasia, M.. Universidad Nacional de Rosario; Argentina | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.language | eng | - |
| dc.publisher | Biomed Central | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/url/http://jbiomedsci.biomedcentral.com/articles/10.1186/1423-0127-17-8 | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1186/1423-0127-17-8 | - |
| dc.rights | info:eu-repo/semantics/openAccess | - |
| dc.rights | https://creativecommons.org/licenses/by/2.5/ar/ | - |
| dc.source | reponame:CONICET Digital (CONICET) | - |
| dc.source | instname:Consejo Nacional de Investigaciones Científicas y Técnicas | - |
| dc.source | instacron:CONICET | - |
| dc.subject | Hyaluronic acid | - |
| dc.subject | erythrocyte deformability | - |
| dc.subject | erythrocyte aggregation | - |
| dc.subject | rheumatoid arthritis | - |
| dc.subject | Biofísica | - |
| dc.subject | Ciencias Biológicas | - |
| dc.subject | CIENCIAS NATURALES Y EXACTAS | - |
| dc.title | In vitro and ex vivo effect of hyaluronic acid on erythrocyte flow properties | - |
| dc.type | info:eu-repo/semantics/article | - |
| dc.type | info:eu-repo/semantics/publishedVersion | - |
| dc.type | info:ar-repo/semantics/articulo | - |
| Aparece en las colecciones: | CONICET | |
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