Registro completo de metadatos
| Campo DC | Valor | Lengua/Idioma |
|---|---|---|
| dc.creator | Campello, Iana S. | - |
| dc.creator | Pereira, Alexsandra F. | - |
| dc.creator | Alcântara Neto, Agostinho S. | - |
| dc.creator | Canel, Natalia G. | - |
| dc.creator | Souza Fabjan, Joanna M. G. | - |
| dc.creator | Teixeira, Dárcio I. A. | - |
| dc.creator | Camargo, Luiz S. A. | - |
| dc.creator | Melo, Luciana M. | - |
| dc.creator | Rádis Baptista, Gandhi | - |
| dc.creator | Salamone, Daniel Felipe | - |
| dc.creator | Freitas, Vicente J. F. | - |
| dc.date | 2017-12-11T21:14:06Z | - |
| dc.date | 2017-12-11T21:14:06Z | - |
| dc.date | 2014-12 | - |
| dc.date | 2017-12-11T15:07:36Z | - |
| dc.date.accessioned | 2019-04-29T15:46:16Z | - |
| dc.date.available | 2019-04-29T15:46:16Z | - |
| dc.date.issued | 2014-12 | - |
| dc.identifier | Campello, Iana S.; Pereira, Alexsandra F.; Alcântara Neto, Agostinho S.; Canel, Natalia G. ; Souza Fabjan, Joanna M. G.; et al.; Effect of crotamine, a cell-penetrating peptide, on blastocyst production and gene expression of in vitro fertilized bovine embryos; Cambridge University Press; Zygote; 24; 1; 12-2014; 48-57 | - |
| dc.identifier | 0967-1994 | - |
| dc.identifier | http://hdl.handle.net/11336/30206 | - |
| dc.identifier | CONICET Digital | - |
| dc.identifier | CONICET | - |
| dc.identifier.uri | http://rodna.bn.gov.ar:8080/jspui/handle/bnmm/301565 | - |
| dc.description | The present study investigated the effects of crotamine, a cell-penetrating peptide from rattlesnake venom, at different exposure times and concentrations, on both developmental competence and gene expression (ATP1A1, AQP3, GLUT1 and GLUT3) of in vitro fertilized (IVF) bovine embryos. In Experiment 1, presumptive zygotes were exposed to 0.1 μM crotamine for 6, 12 or 24 h and control groups (vehicle and IVF) were included. In Experiment 2, presumptive zygotes were exposed to 0 (vehicle), 0.1, 1 and 10 μM crotamine for 24 h. Additionally, to visualize crotamine uptake, embryos were exposed to rhodamine B-labelled crotamine and subjected to confocal microscopy. In Experiment 1, no difference (P > 0.05) was observed among different exposure times and control groups for cleavage and blastocyst rates and total cells number per blastocyst. Within each exposure time, mRNA levels were similar (P > 0.05) in embryos cultured with or without crotamine. In Experiment 2, concentrations as high as 10 μM crotamine did not affect (P > 0.05) the blastocyst rate. Crotamine at 0.1 and 10 μM did not alter mRNA levels when compared with the control (P > 0.05). Remarkably, only 1 μM crotamine decreased both ATP1A1 and AQP3 expression levels relative to the control group (P < 0.05). Also, it was possible to visualize the intracellular localization of crotamine. These results indicate that crotamine can translocate intact IVF bovine embryos and its application in the culture medium is possible at concentrations from 0.1–10 μM for 6–24 h. | - |
| dc.description | Fil: Campello, Iana S.. Universidade Estadual Do Ceara; Brasil | - |
| dc.description | Fil: Pereira, Alexsandra F.. Universidade Estadual Do Ceara; Brasil | - |
| dc.description | Fil: Alcântara Neto, Agostinho S.. Universidade Estadual Do Ceara; Brasil | - |
| dc.description | Fil: Canel, Natalia G.. Universidad de Buenos Aires; Argentina | - |
| dc.description | Fil: Souza Fabjan, Joanna M. G.. Universidade Estadual Do Ceara; Brasil | - |
| dc.description | Fil: Teixeira, Dárcio I. A.. Universidade Estadual Do Ceara; Brasil | - |
| dc.description | Fil: Camargo, Luiz S. A.. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; Brasil | - |
| dc.description | Fil: Melo, Luciana M.. Universidade Estadual Do Ceara; Brasil | - |
| dc.description | Fil: Rádis Baptista, Gandhi. Universidade Federal do Ceará; Brasil | - |
| dc.description | Fil: Salamone, Daniel Felipe. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | - |
| dc.description | Fil: Freitas, Vicente J. F.. Universidade Estadual Do Ceara; Brasil | - |
| dc.format | application/pdf | - |
| dc.format | application/pdf | - |
| dc.language | eng | - |
| dc.publisher | Cambridge University Press | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1017/S0967199414000707 | - |
| dc.relation | info:eu-repo/semantics/altIdentifier/url/https://www.cambridge.org/core/journals/zygote/article/effect-of-crotamine-a-cellpenetrating-peptide-on-blastocyst-production-and-gene-expression-of-in-vitro-fertilized-bovine-embryos/4DFCDCA8AEB3D413F9DD445FA1B638C5 | - |
| dc.rights | info:eu-repo/semantics/restrictedAccess | - |
| dc.rights | https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ | - |
| dc.source | reponame:CONICET Digital (CONICET) | - |
| dc.source | instname:Consejo Nacional de Investigaciones Científicas y Técnicas | - |
| dc.source | instacron:CONICET | - |
| dc.subject | Crotamina | - |
| dc.subject | Preimplantation embryo | - |
| dc.subject | Otras Biotecnología Agropecuaria | - |
| dc.subject | Biotecnología Agropecuaria | - |
| dc.subject | CIENCIAS AGRÍCOLAS | - |
| dc.title | Effect of crotamine, a cell-penetrating peptide, on blastocyst production and gene expression of in vitro fertilized bovine embryos | - |
| dc.type | info:eu-repo/semantics/article | - |
| dc.type | info:eu-repo/semantics/publishedVersion | - |
| dc.type | info:ar-repo/semantics/articulo | - |
| Aparece en las colecciones: | CONICET | |
Ficheros en este ítem:
No hay ficheros asociados a este ítem.